FastQCFastQC Report
Fri 10 Feb 2017
ERR1631785.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631785.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639760
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12480.19507315243216206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12160.19007127672877328No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA11660.18225584594222832No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA11290.17647242716018507No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9850.15396398649493562No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG9790.1530261348005502No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA9410.14708640740277604No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC9360.14630486432412154No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT9320.14567962986119795No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8530.13333124921845693No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC8370.13083031136676251No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT8320.13004876828810805No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA7690.12020132549706139No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA7450.11644991871951982No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA7040.11004126547455295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6850.029.1678831
TACACCA400.001930256623.1255
CGAACTA450.00382398120.55555724
TATATAC450.00382398120.5555573
TGCGGGT2700.020.55555521
AACGTCA2750.020.18181828
GCGGGTA2750.020.18181822
GTAACGT2750.020.18181826
CGGGTAA2800.019.8214323
TTTAGAG951.6732156E-719.4736843
GGTAACG3000.018.525
GTTTATA500.007031556718.4999981
CGTTAGA500.007031556718.4999981
CCATACA500.007031556718.4999982
GTATCAA11200.018.1696432
TATACAC2450.018.12244837
CTTATAC6750.018.08888837
TTAGCCG3150.017.6190474
TTCTGCG3150.017.61904718
TCTTATA12450.017.53413637
Produced by FastQC (version 0.11.5)