##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631785.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	639760
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.606056958859575	34.0	31.0	34.0	31.0	34.0
2	32.76521351756909	34.0	31.0	34.0	31.0	34.0
3	32.85717769163436	34.0	31.0	34.0	31.0	34.0
4	36.288931786920095	37.0	37.0	37.0	35.0	37.0
5	36.206510253845195	37.0	35.0	37.0	35.0	37.0
6	36.253754532949856	37.0	37.0	37.0	35.0	37.0
7	36.23941634362886	37.0	37.0	37.0	35.0	37.0
8	36.21917906715018	37.0	37.0	37.0	35.0	37.0
9	38.04357727897962	39.0	38.0	39.0	35.0	39.0
10	37.97417156433663	39.0	38.0	39.0	35.0	39.0
11	38.05264474177817	39.0	38.0	39.0	35.0	39.0
12	37.98317494060272	39.0	38.0	39.0	35.0	39.0
13	38.04346942603476	39.0	38.0	39.0	35.0	39.0
14	39.42920626484932	40.0	39.0	41.0	37.0	41.0
15	39.42752125797174	40.0	39.0	41.0	37.0	41.0
16	39.38747655370764	40.0	39.0	41.0	37.0	41.0
17	39.35849224709266	40.0	39.0	41.0	36.0	41.0
18	39.3743153682631	40.0	39.0	41.0	36.0	41.0
19	39.401239527322744	40.0	39.0	41.0	37.0	41.0
20	39.38624015255721	40.0	39.0	41.0	37.0	41.0
21	39.35500187570339	40.0	39.0	41.0	36.0	41.0
22	39.32749468550706	40.0	39.0	41.0	36.0	41.0
23	39.264314743028635	40.0	39.0	41.0	36.0	41.0
24	39.27771195448293	40.0	39.0	41.0	36.0	41.0
25	39.224443541328	40.0	39.0	41.0	36.0	41.0
26	39.15467519069651	40.0	39.0	41.0	36.0	41.0
27	39.066353007377764	40.0	39.0	41.0	35.0	41.0
28	39.05707609103414	40.0	39.0	41.0	35.0	41.0
29	39.020865637113914	40.0	39.0	41.0	35.0	41.0
30	38.97333218707015	40.0	38.0	41.0	35.0	41.0
31	38.884616106039765	40.0	38.0	41.0	35.0	41.0
32	38.842783231211705	40.0	38.0	41.0	35.0	41.0
33	38.77917344004001	40.0	38.0	41.0	35.0	41.0
34	38.74030730273853	40.0	38.0	41.0	35.0	41.0
35	38.67044673002376	40.0	38.0	41.0	35.0	41.0
36	38.62038733274978	40.0	38.0	41.0	35.0	41.0
37	38.57693822683506	40.0	38.0	41.0	35.0	41.0
38	38.53912873577592	40.0	38.0	41.0	35.0	41.0
39	38.4794594848068	40.0	38.0	41.0	35.0	41.0
40	38.16069776166062	40.0	38.0	41.0	34.0	41.0
41	38.22143147430286	40.0	37.0	41.0	34.0	41.0
42	38.203924909341005	40.0	37.0	41.0	34.0	41.0
43	37.73081468050519	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	1.0
14	0.0
15	0.0
16	1.0
17	0.0
18	5.0
19	9.0
20	23.0
21	35.0
22	96.0
23	159.0
24	297.0
25	521.0
26	865.0
27	1297.0
28	2046.0
29	3133.0
30	4409.0
31	6147.0
32	8313.0
33	11239.0
34	16557.0
35	24440.0
36	37489.0
37	67327.0
38	152224.0
39	303125.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.88420657746655	16.68813304989371	12.799174690508941	27.628485682130798
2	19.964674252844816	19.362729773665123	33.590408903338755	27.08218707015131
3	20.675722145804677	20.30276978867075	29.287701638114292	29.73380642741028
4	15.301831936976365	15.974896836313619	33.79861197949231	34.924659247217704
5	16.914780542703514	32.80730273852695	33.478804551706894	16.79911216706265
6	34.37445291984494	33.40627735400775	15.762786044766788	16.45648368138052
7	30.562554708015504	28.02128923346255	20.521914467925473	20.894241590596472
8	27.508440665249466	31.218894585469553	20.09050268850819	21.18216206077279
9	28.267318994622986	13.385488308115542	18.423627610353883	39.923565086907594
10	19.090909090909093	24.169376016006	30.34841190446417	26.391302988620737
11	38.33828310616481	19.929348505689635	19.544985619607353	22.187382768538203
12	22.8809240965362	24.034325372014507	27.360260097536575	25.724490433912717
13	33.979304739277225	18.123515068150557	21.66171689383519	26.235463298737027
14	23.71858196823809	20.246186069776165	23.997436538702015	32.03779542328373
15	28.49631111666875	24.811335500812806	20.84797424034013	25.844379142178315
16	25.422971114167815	24.322714768038015	23.056146054770537	27.19816806302363
17	26.02210203826435	24.18610103788921	23.05661498061773	26.73518194322871
18	26.346755033137427	22.2860135050644	24.1825059397274	27.184725522070774
19	27.22708515693385	23.046767537826685	24.19673002375891	25.529417281480555
20	28.44363511316744	21.94166562460923	23.83675128173065	25.777947980492684
21	26.838658246842567	22.937664124046517	23.33500062523446	26.888677003876456
22	27.418250593972736	23.35063148680755	22.83059272227085	26.400525196948855
23	27.163154933099914	22.783074903088657	23.558521945729648	26.49524821808178
24	26.900556458672003	22.64208453169939	23.73218081780668	26.725178191821936
25	26.91649993747655	22.469676128548205	24.147023883956482	26.466800050018758
26	26.675940977866702	23.392678504439164	23.7135800925347	26.21780042515943
27	26.15949731149181	22.657402776041017	24.729898712017008	26.45320120045017
28	25.7684131549331	23.28623233712642	23.501156683756406	27.44419782418407
29	26.364886832562213	23.790796548705764	23.601037889208452	26.243278729523574
30	26.201544329123422	22.71554958109291	24.371170438914593	26.711735650869077
31	26.86601225459547	22.950168813304987	23.82315243216206	26.360666499937473
32	25.3166812554708	23.04223458797049	23.87160810303864	27.76947605352007
33	25.268694510441414	22.466237339002127	25.477835438289357	26.7872327122671
34	25.791859447292737	22.19269726147305	24.92153307490309	27.093910216331125
35	25.121295485807178	23.610260097536575	25.37607852944854	25.892365887207703
36	25.612417156433665	22.637864199074652	25.37045141928223	26.379267225209453
37	25.600694010253843	22.391521820682755	25.50253219957484	26.505251969488558
38	25.0683068650744	21.883675128173067	26.17184569213455	26.87617231461798
39	24.77397774165312	21.118231836938854	26.937288983368763	27.170501438039263
40	24.43166187320245	22.059209703638867	27.499218456921348	26.009909966237338
41	23.1272664749281	21.316900087532826	28.425972239589846	27.12986119794923
42	22.042015755908466	21.859447292734778	28.94694885582093	27.15158809553583
43	21.660622733525074	22.130173815180694	28.789077153932723	27.42012629736151
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10.0
1	11.5
2	13.0
3	36.0
4	59.0
5	59.0
6	70.5
7	82.0
8	100.0
9	118.0
10	180.0
11	242.0
12	242.0
13	491.0
14	740.0
15	1436.0
16	2132.0
17	2135.5
18	2139.0
19	2139.0
20	2429.0
21	2719.0
22	2311.0
23	1903.0
24	2124.0
25	2345.0
26	2345.0
27	2723.5
28	3102.0
29	3912.0
30	4722.0
31	5833.5
32	6945.0
33	6945.0
34	8947.5
35	10950.0
36	12551.5
37	14153.0
38	16770.0
39	19387.0
40	19387.0
41	22701.0
42	26015.0
43	29986.5
44	33958.0
45	42168.5
46	50379.0
47	50379.0
48	54327.0
49	58275.0
50	63455.5
51	68636.0
52	67684.5
53	66733.0
54	66733.0
55	61050.0
56	55367.0
57	51895.5
58	48424.0
59	45358.0
60	42292.0
61	42292.0
62	38577.0
63	34862.0
64	29902.5
65	24943.0
66	21999.0
67	19055.0
68	19055.0
69	16402.0
70	13749.0
71	11602.0
72	9455.0
73	7693.5
74	5932.0
75	5932.0
76	4922.5
77	3913.0
78	3121.0
79	2329.0
80	1978.5
81	1628.0
82	1628.0
83	1308.0
84	988.0
85	764.0
86	540.0
87	416.0
88	292.0
89	292.0
90	234.0
91	176.0
92	110.5
93	45.0
94	27.0
95	9.0
96	9.0
97	6.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	639760.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.31057057529499
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.74380819994074	51.448247582124374
2	6.954699256211355	8.249743621309502
3	2.237905157848548	3.981942954161789
4	1.057380978515687	2.508554766049165
5	0.6355193924219229	1.8846508888104523
6	0.4166085800972658	1.482557555527939
7	0.3000761810230451	1.2458382658772618
8	0.22692116790994882	1.0767059155481105
9	0.16533047591984817	0.8825260374262136
>10	1.0785780124218178	12.055558590002164
>50	0.1081062131150971	4.4953429781595755
>100	0.06898706469719397	7.781212712005178
>500	0.005022046855347839	2.161056053892442
>1k	0.0010572730221784924	0.7460620791058492
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1248	0.19507315243216206	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1216	0.19007127672877328	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1166	0.18225584594222832	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1129	0.17647242716018507	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	985	0.15396398649493562	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	979	0.1530261348005502	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	941	0.14708640740277604	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	936	0.14630486432412154	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	932	0.14567962986119795	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	853	0.13333124921845693	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	837	0.13083031136676251	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	832	0.13004876828810805	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	769	0.12020132549706139	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	745	0.11644991871951982	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	704	0.11004126547455295	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	1.563086157308991E-4
4	0.0	0.0	0.0	0.0	1.563086157308991E-4
5	0.0	0.0	0.0	0.0	1.563086157308991E-4
6	0.0	0.0	0.0	0.0	3.126172314617982E-4
7	0.0	0.0	0.0	0.0	3.126172314617982E-4
8	3.126172314617982E-4	0.0	0.0	0.0	6.252344629235964E-4
9	3.126172314617982E-4	0.0	0.0	1.563086157308991E-4	6.252344629235964E-4
10	3.126172314617982E-4	0.0	0.0	1.563086157308991E-4	6.252344629235964E-4
11	3.126172314617982E-4	0.0	0.0	1.563086157308991E-4	6.252344629235964E-4
12	3.126172314617982E-4	0.0	0.0	1.563086157308991E-4	7.815430786544955E-4
13	3.126172314617982E-4	0.0	0.0	1.563086157308991E-4	7.815430786544955E-4
14	4.6892584719269726E-4	0.0	0.0	1.563086157308991E-4	7.815430786544955E-4
15	4.6892584719269726E-4	0.0	0.0	3.126172314617982E-4	7.815430786544955E-4
16	4.6892584719269726E-4	0.0	0.0	6.252344629235964E-4	9.378516943853945E-4
17	4.6892584719269726E-4	0.0	0.0	0.0012504689258471928	9.378516943853945E-4
18	4.6892584719269726E-4	0.0	0.0	0.0017193947730398899	9.378516943853945E-4
19	4.6892584719269726E-4	0.0	0.0	0.002344629235963486	9.378516943853945E-4
20	4.6892584719269726E-4	0.0	0.0	0.0026572464674252845	9.378516943853945E-4
21	4.6892584719269726E-4	0.0	0.0	0.005001875703388771	9.378516943853945E-4
22	4.6892584719269726E-4	0.0	0.0	0.0068775790921595595	9.378516943853945E-4
23	4.6892584719269726E-4	0.0	0.0	0.007815430786544954	9.378516943853945E-4
24	4.6892584719269726E-4	0.0	0.0	0.009847442791046643	9.378516943853945E-4
25	4.6892584719269726E-4	0.0	0.0	0.011566837564086533	9.378516943853945E-4
26	4.6892584719269726E-4	0.0	0.0	0.015943478804551707	0.0010941603101162936
27	4.6892584719269726E-4	0.0	0.0	0.03329373515068151	0.0010941603101162936
28	4.6892584719269726E-4	0.0	0.0	0.11801300487682881	0.0010941603101162936
29	4.6892584719269726E-4	0.0	0.0	0.2554082781042891	0.0010941603101162936
30	4.6892584719269726E-4	0.0	0.0	0.43438164311616856	0.0010941603101162936
31	4.6892584719269726E-4	0.0	0.0	0.9644241590596474	0.0010941603101162936
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	685	0.0	29.167883	1
TACACCA	40	0.0019302566	23.125	5
CGAACTA	45	0.003823981	20.555557	24
TATATAC	45	0.003823981	20.555557	3
TGCGGGT	270	0.0	20.555555	21
AACGTCA	275	0.0	20.181818	28
GCGGGTA	275	0.0	20.181818	22
GTAACGT	275	0.0	20.181818	26
CGGGTAA	280	0.0	19.82143	23
TTTAGAG	95	1.6732156E-7	19.473684	3
GGTAACG	300	0.0	18.5	25
GTTTATA	50	0.0070315567	18.499998	1
CGTTAGA	50	0.0070315567	18.499998	1
CCATACA	50	0.0070315567	18.499998	2
GTATCAA	1120	0.0	18.169643	2
TATACAC	245	0.0	18.122448	37
CTTATAC	675	0.0	18.088888	37
TTAGCCG	315	0.0	17.619047	4
TTCTGCG	315	0.0	17.619047	18
TCTTATA	1245	0.0	17.534136	37
>>END_MODULE