FastQCFastQC Report
Fri 10 Feb 2017
ERR1631784.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631784.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1138655
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31910.2802429181797823No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29140.255915971036003No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23730.20840377462883838No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA16430.14429304749902297No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA16100.1413948913410998No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA15990.14042883928845876No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA14530.12760669386249565No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG14270.12532329810170773No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC13840.12154691280501996No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA13710.12040521492462598No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12850.11285244433125047No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT12740.11188639227860941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15800.029.389241
ATACGGC905.456968E-1226.72222129
GTGTTAT451.3232017E-424.6666661
TCTATAC502.702081E-422.1999993
GTAACGT5150.019.39805826
GCAGTCG3200.019.0781259
GTATCAA24250.018.9195862
TACGGCT1306.9667294E-1018.530
TGCGGGT5700.018.521
GTGTAAG801.6166488E-518.51
TAAGGTA1103.851528E-818.55
TGTTACG500.00703484418.49999816
CTTATAC12550.018.42629437
TAGTTCC1451.546141E-1017.8620684
ATAACGC1451.546141E-1017.86206835
GCGGTAA5300.017.80188823
TTCTGCG5950.017.72268918
GGTAACG5750.017.69565225
AGTCGGT3250.017.64615411
GACCGTT1054.797166E-717.6190477
Produced by FastQC (version 0.11.5)