##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631784.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1138655
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.59649323104891	34.0	31.0	34.0	31.0	34.0
2	32.75930022702223	34.0	31.0	34.0	31.0	34.0
3	32.8512692606628	34.0	31.0	34.0	31.0	34.0
4	36.28818562251077	37.0	37.0	37.0	35.0	37.0
5	36.20424448142765	37.0	35.0	37.0	35.0	37.0
6	36.25375552735464	37.0	37.0	37.0	35.0	37.0
7	36.239438635934505	37.0	37.0	37.0	35.0	37.0
8	36.221960119614806	37.0	37.0	37.0	35.0	37.0
9	38.045529155011835	39.0	38.0	39.0	35.0	39.0
10	37.97738735613509	39.0	38.0	39.0	35.0	39.0
11	38.053417409136216	39.0	38.0	39.0	35.0	39.0
12	37.99180260921877	39.0	38.0	39.0	35.0	39.0
13	38.04503295554843	39.0	38.0	39.0	35.0	39.0
14	39.431296573589016	40.0	39.0	41.0	37.0	41.0
15	39.42868472013033	40.0	39.0	41.0	37.0	41.0
16	39.38189003692953	40.0	39.0	41.0	36.0	41.0
17	39.35770711936451	40.0	39.0	41.0	36.0	41.0
18	39.377127400310016	40.0	39.0	41.0	36.0	41.0
19	39.41077674976178	40.0	39.0	41.0	37.0	41.0
20	39.401226886106855	40.0	39.0	41.0	37.0	41.0
21	39.36659655470709	40.0	39.0	41.0	36.0	41.0
22	39.34281498785848	40.0	39.0	41.0	36.0	41.0
23	39.27969929434289	40.0	39.0	41.0	36.0	41.0
24	39.294436857520495	40.0	39.0	41.0	36.0	41.0
25	39.23926386833589	40.0	39.0	41.0	36.0	41.0
26	39.175298927243105	40.0	39.0	41.0	36.0	41.0
27	39.07061752681892	40.0	39.0	41.0	35.0	41.0
28	39.057783964414156	40.0	39.0	41.0	35.0	41.0
29	39.027661583183665	40.0	39.0	41.0	35.0	41.0
30	38.97751118644366	40.0	38.0	41.0	35.0	41.0
31	38.882724793725934	40.0	38.0	41.0	35.0	41.0
32	38.8269010367495	40.0	38.0	41.0	35.0	41.0
33	38.76155288476316	40.0	38.0	41.0	35.0	41.0
34	38.72980577962596	40.0	38.0	41.0	35.0	41.0
35	38.65415512161278	40.0	38.0	41.0	35.0	41.0
36	38.604704673496364	40.0	38.0	41.0	35.0	41.0
37	38.552699456815276	40.0	38.0	41.0	35.0	41.0
38	38.50770514334895	40.0	38.0	41.0	35.0	41.0
39	38.43953348468149	40.0	38.0	41.0	34.0	41.0
40	38.125079150401135	40.0	38.0	41.0	34.0	41.0
41	38.1847144218398	40.0	37.0	41.0	34.0	41.0
42	38.158575687982754	40.0	37.0	41.0	34.0	41.0
43	37.68821548230149	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	1.0
13	1.0
14	0.0
15	2.0
16	1.0
17	5.0
18	11.0
19	16.0
20	47.0
21	91.0
22	164.0
23	294.0
24	615.0
25	1003.0
26	1579.0
27	2477.0
28	3762.0
29	5705.0
30	8019.0
31	11033.0
32	14680.0
33	19929.0
34	28936.0
35	43456.0
36	66400.0
37	120992.0
38	268700.0
39	540732.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.531232023747314	17.402549499189835	13.092376531960953	26.973841945101896
2	19.564310524258886	20.179685681791238	34.2625290364509	25.99347475749898
3	20.487329349100474	21.07925578862781	29.89351471692479	28.539900145346923
4	15.003666606654342	16.36114538644278	34.56112694363087	34.07406106327202
5	16.157220580421637	33.697037294000374	33.91597981829439	16.229762307283593
6	33.29305189016866	34.54031291304214	15.838159934308461	16.328475262480737
7	29.54362822804098	28.957673746657242	20.815435755342925	20.683262269958856
8	26.754460306238503	32.20290606022017	20.308170604792497	20.73446302874883
9	27.839863698837664	13.835182737528049	18.791820173801547	39.53313338983274
10	18.606513825522217	24.722940662448238	30.995516640246606	25.675028871782935
11	36.998212803702614	20.82421804673057	20.215693076480584	21.961876073086227
12	22.329151498917582	24.91992745827314	27.71418910908045	25.036731933728827
13	33.00631007636202	18.870421681720977	22.512701388919382	25.610566852997618
14	23.442131286473955	20.910284502329503	24.545011438934534	31.10257277226201
15	27.962025372039818	25.556028823480336	21.47858657802407	25.003359226455775
16	24.68078566378754	25.224585146510574	23.611629510255522	26.482999679446362
17	25.291945321453817	24.90868612529695	23.620675270384798	26.178693282864433
18	25.191388085065274	23.134224150423087	25.185767418577182	26.48862034593446
19	26.671292006797493	23.58203318827915	25.33436378885615	24.4123110160672
20	27.635236309505512	22.59543057379101	24.877069876301423	24.892263240402055
21	25.907496124813928	23.953611936890454	23.971264342579623	26.167627595716
22	26.379807755641526	24.27917147863049	23.60073946893484	25.740281296793142
23	26.01499137139871	23.742222183189813	24.452006973139362	25.790779472272114
24	26.278635758855845	23.402435329401793	24.53596567880526	25.782963232937107
25	26.11291392037096	23.473484066727853	24.96129205070895	25.45230996219224
26	25.918649634876235	24.049075444274166	24.404494776732196	25.627780144117402
27	25.737558786462976	23.391193996425606	25.270340884640213	25.600906332471208
28	25.038664037834113	24.2000430332278	24.27565856207543	26.48563436686266
29	25.542767563484993	24.530608481058795	24.527359033245364	25.39926492221085
30	25.360798485932968	23.969946998871475	25.25251283312329	25.41674168207227
31	26.030536027154845	23.918570594253747	24.628355384203292	25.422537994388115
32	24.08499501604964	23.955544040995736	24.90078206304807	27.058678879906555
33	24.4723818891587	23.515024304991417	26.190988490807136	25.82160531504275
34	24.915360666751564	23.33955412306625	25.711036266472288	26.0340489437099
35	24.626423280098013	24.33862759132485	25.81923409636808	25.21571503220905
36	24.79109124361637	23.590815479666798	26.062854859461382	25.555238417255445
37	25.04867585001603	23.119821192547345	25.87772415700981	25.953778800426818
38	24.363920590521275	22.658311780126553	26.87231865666071	26.105448972691462
39	23.966082790660913	22.03380303955105	27.6960975888219	26.304016580966138
40	23.71288932995508	22.75825425611796	28.138900720587007	25.38995569333995
41	22.44086224536844	22.234214929017128	29.058582274701294	26.266340550913135
42	21.64404494776732	22.535008409044003	29.316518172756457	26.504428470432224
43	21.05396278943139	23.00196284212514	29.227553560999603	26.71652080744387
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	44.0
1	41.0
2	38.0
3	102.5
4	167.0
5	167.0
6	227.0
7	287.0
8	307.5
9	328.0
10	535.5
11	743.0
12	743.0
13	1420.5
14	2098.0
15	4052.5
16	6007.0
17	5932.5
18	5858.0
19	5858.0
20	6682.5
21	7507.0
22	6793.0
23	6079.0
24	6779.0
25	7479.0
26	7479.0
27	8600.5
28	9722.0
29	12356.5
30	14991.0
31	17197.0
32	19403.0
33	19403.0
34	22667.5
35	25932.0
36	28891.5
37	31851.0
38	36827.0
39	41803.0
40	41803.0
41	47173.0
42	52543.0
43	56845.5
44	61148.0
45	72885.5
46	84623.0
47	84623.0
48	91335.0
49	98047.0
50	107848.5
51	117650.0
52	117387.5
53	117125.0
54	117125.0
55	105960.5
56	94796.0
57	88362.5
58	81929.0
59	74923.0
60	67917.0
61	67917.0
62	61741.5
63	55566.0
64	46812.5
65	38059.0
66	33245.5
67	28432.0
68	28432.0
69	24241.0
70	20050.0
71	17347.5
72	14645.0
73	11992.5
74	9340.0
75	9340.0
76	7777.0
77	6214.0
78	5134.5
79	4055.0
80	3323.0
81	2591.0
82	2591.0
83	2077.0
84	1563.0
85	1274.0
86	985.0
87	792.0
88	599.0
89	599.0
90	463.0
91	327.0
92	209.5
93	92.0
94	53.0
95	14.0
96	14.0
97	9.0
98	4.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1138655.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.08984491221153
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.68875749264913	45.6769377833662
2	6.037495117030481	6.2898436860870435
3	1.9456518158682181	3.040461040252147
4	0.9919717584373414	2.066866202171795
5	0.6046459183210338	1.5747956056072185
6	0.4032403826715182	1.2602837397420128
7	0.30269831017032284	1.1037255622372435
8	0.21481862162569593	0.8951894947790041
9	0.18181894506626797	0.8523828585543427
>10	1.3121278193237047	13.900083083492524
>50	0.1877731835833333	6.844961221841449
>100	0.11918168963034859	11.71851102146436
>500	0.0072795631340198	2.5936685924094482
>1k	0.002539382488611558	2.182290107995261
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3191	0.2802429181797823	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2914	0.255915971036003	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2373	0.20840377462883838	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1643	0.14429304749902297	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1610	0.1413948913410998	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1599	0.14042883928845876	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1453	0.12760669386249565	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1427	0.12532329810170773	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1384	0.12154691280501996	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1371	0.12040521492462598	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1285	0.11285244433125047	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1274	0.11188639227860941	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	8.78229138764595E-5	0.0
3	0.0	0.0	0.0	8.78229138764595E-5	0.0
4	0.0	0.0	0.0	8.78229138764595E-5	0.0
5	8.78229138764595E-5	0.0	0.0	8.78229138764595E-5	0.0
6	8.78229138764595E-5	0.0	0.0	8.78229138764595E-5	0.0
7	1.75645827752919E-4	0.0	0.0	8.78229138764595E-5	0.0
8	2.634687416293785E-4	0.0	0.0	8.78229138764595E-5	0.0
9	2.634687416293785E-4	0.0	0.0	8.78229138764595E-5	0.0
10	3.51291655505838E-4	0.0	0.0	8.78229138764595E-5	0.0
11	3.51291655505838E-4	8.78229138764595E-5	0.0	8.78229138764595E-5	0.0
12	3.51291655505838E-4	8.78229138764595E-5	0.0	8.78229138764595E-5	0.0
13	3.51291655505838E-4	8.78229138764595E-5	0.0	8.78229138764595E-5	0.0
14	3.51291655505838E-4	8.78229138764595E-5	0.0	1.75645827752919E-4	0.0
15	3.51291655505838E-4	8.78229138764595E-5	0.0	3.51291655505838E-4	0.0
16	3.51291655505838E-4	8.78229138764595E-5	0.0	8.782291387645951E-4	0.0
17	3.51291655505838E-4	8.78229138764595E-5	0.0	0.0011416978803939735	0.0
18	4.3911456938229755E-4	8.78229138764595E-5	0.0	0.0014929895358998116	0.0
19	5.26937483258757E-4	8.78229138764595E-5	0.0	0.002195572846911488	0.0
20	5.26937483258757E-4	8.78229138764595E-5	0.0	0.0029859790717996233	0.0
21	5.26937483258757E-4	8.78229138764595E-5	0.0	0.00526937483258757	0.0
22	5.26937483258757E-4	8.78229138764595E-5	0.0	0.007201478937869679	0.0
23	5.26937483258757E-4	8.78229138764595E-5	0.0	0.009309228870904707	0.0
24	5.26937483258757E-4	8.78229138764595E-5	0.0	0.011504801717816196	0.0
25	5.26937483258757E-4	8.78229138764595E-5	0.0	0.012997791253716006	0.0
26	6.147603971352165E-4	8.78229138764595E-5	0.0	0.016598530722650846	0.0
27	6.147603971352165E-4	8.78229138764595E-5	0.0	0.03609521760322486	0.0
28	6.147603971352165E-4	8.78229138764595E-5	0.0	0.11873657956097325	0.0
29	6.147603971352165E-4	8.78229138764595E-5	0.0	0.2498561899785273	0.0
30	6.147603971352165E-4	8.78229138764595E-5	0.0	0.4205839345543646	0.0
31	6.147603971352165E-4	8.78229138764595E-5	0.0	0.9322402307986176	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1580	0.0	29.38924	1
ATACGGC	90	5.456968E-12	26.722221	29
GTGTTAT	45	1.3232017E-4	24.666666	1
TCTATAC	50	2.702081E-4	22.199999	3
GTAACGT	515	0.0	19.398058	26
GCAGTCG	320	0.0	19.078125	9
GTATCAA	2425	0.0	18.919586	2
TACGGCT	130	6.9667294E-10	18.5	30
TGCGGGT	570	0.0	18.5	21
GTGTAAG	80	1.6166488E-5	18.5	1
TAAGGTA	110	3.851528E-8	18.5	5
TGTTACG	50	0.007034844	18.499998	16
CTTATAC	1255	0.0	18.426294	37
TAGTTCC	145	1.546141E-10	17.862068	4
ATAACGC	145	1.546141E-10	17.862068	35
GCGGTAA	530	0.0	17.801888	23
TTCTGCG	595	0.0	17.722689	18
GGTAACG	575	0.0	17.695652	25
AGTCGGT	325	0.0	17.646154	11
GACCGTT	105	4.797166E-7	17.619047	7
>>END_MODULE