FastQCFastQC Report
Fri 10 Feb 2017
ERR1631783.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631783.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences935099
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30400.32509926756418306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29010.3102345313170049No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23710.25355604059035464No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA15120.16169410939376472No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA15050.16094552555397879No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA14730.1575234280006716No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA13700.14650855150096406No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA13160.14073376187975817No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC12580.1345312100643889No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG12370.13228545854503107No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT12210.13057440976837748No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11880.12704537166652943No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC11520.12319551191905885No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA10760.11506803022995427No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT9950.10640584579814542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13950.027.8494631
TGAACGG400.001930918923.1250025
CAAGACA705.1002444E-621.1428594
CCTTAGT450.00382528320.5555552
CGTTATT656.900371E-519.92307710
CTTATAC10400.018.8557737
TATACAC3550.018.76056537
TTGGTCG500.00703392518.513
TGTTACG801.6161446E-518.516
AAGGGCG701.21888865E-418.55
TAGGACA752.0667465E-417.2666664
GCGGTAA5750.017.05217423
TCTTATA19000.016.84473637
CAGTCGG3350.016.56716310
ATATCCC1352.21753E-816.4444433
GTATCAA23650.016.3488372
TATTAGA803.3817207E-416.18752
CGCGGTA6400.015.89843822
GCGAAAT700.002592008815.85714312
TAGCGAA700.002592008815.85714310
Produced by FastQC (version 0.11.5)