##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631783.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	935099
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.584547732379136	34.0	31.0	34.0	31.0	34.0
2	32.745195963208175	34.0	31.0	34.0	31.0	34.0
3	32.830663919007506	34.0	31.0	34.0	31.0	34.0
4	36.27186640131152	37.0	37.0	37.0	35.0	37.0
5	36.18745394872629	37.0	35.0	37.0	35.0	37.0
6	36.239378932070295	37.0	37.0	37.0	35.0	37.0
7	36.22649794299855	37.0	37.0	37.0	35.0	37.0
8	36.206783452875044	37.0	36.0	37.0	35.0	37.0
9	38.03018717804211	39.0	38.0	39.0	35.0	39.0
10	37.96442729593337	39.0	38.0	39.0	35.0	39.0
11	38.036714829125046	39.0	38.0	39.0	35.0	39.0
12	37.977671882870155	39.0	38.0	39.0	35.0	39.0
13	38.02941613668713	39.0	38.0	39.0	35.0	39.0
14	39.397587848987115	40.0	39.0	41.0	37.0	41.0
15	39.40462667589207	40.0	39.0	41.0	37.0	41.0
16	39.358232657718595	40.0	39.0	41.0	36.0	41.0
17	39.33183652212226	40.0	39.0	41.0	36.0	41.0
18	39.35774073119531	40.0	39.0	41.0	36.0	41.0
19	39.38768622359772	40.0	39.0	41.0	37.0	41.0
20	39.37622861322705	40.0	39.0	41.0	36.0	41.0
21	39.34845829158196	40.0	39.0	41.0	36.0	41.0
22	39.31661353503747	40.0	39.0	41.0	36.0	41.0
23	39.249767136955555	40.0	39.0	41.0	36.0	41.0
24	39.25945060362593	40.0	39.0	41.0	36.0	41.0
25	39.20872977085849	40.0	39.0	41.0	36.0	41.0
26	39.1374763527712	40.0	39.0	41.0	36.0	41.0
27	39.02968669627494	40.0	39.0	41.0	35.0	41.0
28	39.0144380434585	40.0	39.0	41.0	35.0	41.0
29	38.984832622000454	40.0	39.0	41.0	35.0	41.0
30	38.92548275637125	40.0	38.0	41.0	35.0	41.0
31	38.82842137570461	40.0	38.0	41.0	35.0	41.0
32	38.76243798784942	40.0	38.0	41.0	35.0	41.0
33	38.698930273692945	40.0	38.0	41.0	35.0	41.0
34	38.65770362282496	40.0	38.0	41.0	35.0	41.0
35	38.58599142978444	40.0	38.0	41.0	35.0	41.0
36	38.52862103370873	40.0	38.0	41.0	35.0	41.0
37	38.473602260295436	40.0	38.0	41.0	35.0	41.0
38	38.426983666970024	40.0	38.0	41.0	35.0	41.0
39	38.352428994149285	40.0	38.0	41.0	34.0	41.0
40	38.03373653484818	40.0	37.0	41.0	34.0	41.0
41	38.078554249336165	40.0	37.0	41.0	34.0	41.0
42	38.048387389998275	40.0	37.0	41.0	34.0	41.0
43	37.565996755423754	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	1.0
15	0.0
16	1.0
17	4.0
18	8.0
19	23.0
20	55.0
21	79.0
22	167.0
23	300.0
24	557.0
25	873.0
26	1424.0
27	2237.0
28	3474.0
29	4904.0
30	6970.0
31	9149.0
32	12389.0
33	17137.0
34	24235.0
35	36110.0
36	55625.0
37	101860.0
38	222707.0
39	434809.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.49988503891032	17.302552991715316	13.339015441145804	26.858546528228562
2	19.71459706405418	20.333782840105698	33.974370628136704	25.977249467703416
3	20.63695929521901	21.080655631114993	29.92271406556953	28.35967100809647
4	15.142781673384315	16.58712072197703	34.66777314487557	33.60232445976308
5	16.33666595729436	33.519659415741	33.806046204733406	16.33762842223123
6	32.67397355787997	34.63740202908997	16.146632602537274	16.541991810492792
7	29.329728723910513	28.982065000604212	20.87746858888738	20.81073768659789
8	26.77534678146378	31.7871155888307	20.542530790857437	20.89500683884808
9	27.912124812453015	13.781963193202005	18.888374386027575	39.41753760831741
10	18.722723476337798	24.547775155357883	30.7978085742793	25.931692794025018
11	36.78562376817855	20.96815417405002	20.0568068193849	22.189415238386523
12	22.272080282408602	24.995321351001337	27.72562049579777	25.00697787079229
13	33.26460620747108	18.78945437862729	22.529593123294966	25.41634629060666
14	23.445218099901723	21.15957775593814	24.686690927912448	30.70851321624769
15	28.13798325097129	25.350257031608418	21.574827905922263	24.936931811498035
16	24.300314726034355	25.306946109449374	23.89287123609372	26.499867928422553
17	25.100978612959697	24.993503361676144	23.70294482188517	26.20257320347899
18	25.27090714459111	22.814803566253413	25.405331414106957	26.508957875048523
19	26.637286533297544	23.415488627407367	25.573869718607334	24.373355120687755
20	27.927310370345815	22.357204959047117	24.886883634780915	24.828601035826154
21	26.053070316618882	23.64027766044023	24.09327782405927	26.213374198881617
22	26.39934381279415	24.107500917015205	23.822825176799462	25.67033009339118
23	26.090178686962556	23.495159336070298	24.71000396749435	25.70465800947279
24	26.11039045063678	23.287908552998132	24.82656916540388	25.77513183096121
25	25.935542653772487	23.513873932064946	25.094241358401625	25.45634205576094
26	25.81480677446987	24.154554758373177	24.537722743795044	25.492915723361914
27	25.70658293934653	23.250693242105918	25.44211896280501	25.60060485574255
28	25.005801524758343	24.08354623414205	24.3886476191291	26.522004621970506
29	25.375067238869896	24.497833919189304	24.64198977862237	25.48510906331843
30	25.293364659784682	23.704762811210365	25.571410085990898	25.430462443014058
31	26.006871999649235	23.789245844557634	24.785076232570027	25.418805923223104
32	24.112741003893706	23.737486619063862	25.114346181527303	27.03542619551513
33	24.332717712242232	23.314429809036262	26.47569936445232	25.877153114269184
34	24.794593941390165	23.133700282002227	26.089857865316933	25.98184791129068
35	24.59408041287607	24.2226758877937	26.169421633431327	25.013822065898907
36	24.680916138291238	23.29678461852702	26.48457542998121	25.53772381320053
37	24.885707288746968	22.975428270161768	26.25796840762315	25.880896033468115
38	24.339027204606143	22.455697204253237	27.155627372075042	26.049648219065574
39	23.945379045427277	21.713529797379742	28.03050799968773	26.310583157505246
40	23.60006801418887	22.57162075887152	28.41068164975045	25.417629577189153
41	22.13744213179567	21.946660193198795	29.524467462803404	26.39143021220213
42	21.202781737548644	22.440083884166278	29.74166371688987	26.615470661395214
43	20.62466113213681	23.00451609936488	29.5291728469392	26.841649921559107
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	36.0
1	34.5
2	33.0
3	111.0
4	189.0
5	189.0
6	227.5
7	266.0
8	308.5
9	351.0
10	546.0
11	741.0
12	741.0
13	1485.0
14	2229.0
15	4022.5
16	5816.0
17	5677.0
18	5538.0
19	5538.0
20	6252.0
21	6966.0
22	6010.5
23	5055.0
24	5459.0
25	5863.0
26	5863.0
27	6610.0
28	7357.0
29	9076.5
30	10796.0
31	12826.0
32	14856.0
33	14856.0
34	17564.5
35	20273.0
36	22282.0
37	24291.0
38	28021.0
39	31751.0
40	31751.0
41	36204.5
42	40658.0
43	44831.5
44	49005.0
45	60195.5
46	71386.0
47	71386.0
48	77309.0
49	83232.0
50	92075.0
51	100918.0
52	100354.0
53	99790.0
54	99790.0
55	90111.5
56	80433.0
57	74735.5
58	69038.0
59	62654.0
60	56270.0
61	56270.0
62	50839.5
63	45409.0
64	37592.5
65	29776.0
66	25703.0
67	21630.0
68	21630.0
69	18435.0
70	15240.0
71	13172.5
72	11105.0
73	9020.0
74	6935.0
75	6935.0
76	5724.5
77	4514.0
78	3659.0
79	2804.0
80	2282.5
81	1761.0
82	1761.0
83	1454.5
84	1148.0
85	953.5
86	759.0
87	613.5
88	468.0
89	468.0
90	379.5
91	291.0
92	194.0
93	97.0
94	58.5
95	20.0
96	20.0
97	12.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	935099.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.95806904092146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.332094498908	44.50274900963631
2	6.18230516760957	6.30076667126188
3	1.9037755070979694	2.9103817118734074
4	1.030144804206395	2.09976760219584
5	0.6223658462000163	1.5857280879685969
6	0.4420245208452212	1.3514829630606602
7	0.2991473143147992	1.0670778647381838
8	0.237894000381915	0.9698095116706097
9	0.1834604979592099	0.8413913449158523
>10	1.4504821479826366	14.988099139752359
>50	0.1808376565848124	6.413695242685133
>100	0.12407353005299297	11.726927787547574
>500	0.008440376189974777	2.8098777460270106
>1k	0.0029541316664911717	2.4322453166665765
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3040	0.32509926756418306	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2901	0.3102345313170049	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2371	0.25355604059035464	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1512	0.16169410939376472	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1505	0.16094552555397879	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1473	0.1575234280006716	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1370	0.14650855150096406	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1316	0.14073376187975817	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1258	0.1345312100643889	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1237	0.13228545854503107	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1221	0.13057440976837748	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1188	0.12704537166652943	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1152	0.12319551191905885	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1076	0.11506803022995427	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	995	0.10640584579814542	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.0694054854084968E-4	0.0	0.0	0.0	0.0
7	1.0694054854084968E-4	0.0	0.0	0.0	0.0
8	3.2082164562254907E-4	0.0	0.0	0.0	0.0
9	3.2082164562254907E-4	0.0	0.0	1.0694054854084968E-4	0.0
10	3.2082164562254907E-4	0.0	0.0	1.0694054854084968E-4	0.0
11	3.2082164562254907E-4	0.0	0.0	1.0694054854084968E-4	0.0
12	3.2082164562254907E-4	0.0	0.0	1.0694054854084968E-4	0.0
13	3.2082164562254907E-4	0.0	0.0	1.0694054854084968E-4	0.0
14	3.2082164562254907E-4	0.0	0.0	1.0694054854084968E-4	0.0
15	4.277621941633987E-4	0.0	0.0	3.2082164562254907E-4	0.0
16	4.277621941633987E-4	0.0	0.0	0.0010694054854084969	0.0
17	4.277621941633987E-4	0.0	0.0	0.0018179893251944446	0.0
18	4.277621941633987E-4	0.0	0.0	0.002031870422276144	0.0
19	4.277621941633987E-4	0.0	0.0	0.002459632616439543	0.0
20	5.347027427042484E-4	0.0	0.0	0.00342209755330719	0.0
21	5.347027427042484E-4	0.0	0.0	0.004919265232879086	0.0
22	6.416432912450981E-4	0.0	0.0	0.007165016752236929	0.0
23	6.416432912450981E-4	0.0	0.0	0.008448303334727125	0.0
24	6.416432912450981E-4	0.0	0.0	0.010694054854084969	0.0
25	6.416432912450981E-4	0.0	0.0	0.011977341436575166	0.0
26	6.416432912450981E-4	0.0	0.0	0.015185557892800655	0.0
27	6.416432912450981E-4	0.0	0.0	0.041172111188227126	0.0
28	6.416432912450981E-4	0.0	0.0	0.14950288686010785	0.0
29	6.416432912450981E-4	1.0694054854084968E-4	0.0	0.31344274777323045	0.0
30	6.416432912450981E-4	1.0694054854084968E-4	0.0	0.5205865902968563	0.0
31	6.416432912450981E-4	1.0694054854084968E-4	0.0	1.1360294471494463	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1395	0.0	27.849463	1
TGAACGG	40	0.0019309189	23.125002	5
CAAGACA	70	5.1002444E-6	21.142859	4
CCTTAGT	45	0.003825283	20.555555	2
CGTTATT	65	6.900371E-5	19.923077	10
CTTATAC	1040	0.0	18.85577	37
TATACAC	355	0.0	18.760565	37
TTGGTCG	50	0.007033925	18.5	13
TGTTACG	80	1.6161446E-5	18.5	16
AAGGGCG	70	1.21888865E-4	18.5	5
TAGGACA	75	2.0667465E-4	17.266666	4
GCGGTAA	575	0.0	17.052174	23
TCTTATA	1900	0.0	16.844736	37
CAGTCGG	335	0.0	16.567163	10
ATATCCC	135	2.21753E-8	16.444443	3
GTATCAA	2365	0.0	16.348837	2
TATTAGA	80	3.3817207E-4	16.1875	2
CGCGGTA	640	0.0	15.898438	22
GCGAAAT	70	0.0025920088	15.857143	12
TAGCGAA	70	0.0025920088	15.857143	10
>>END_MODULE