FastQCFastQC Report
Fri 10 Feb 2017
ERR1631782.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631782.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058476
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT149221.4097627154512715No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT148091.3990869892184612No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT116031.0961986856574926No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39070.36911559638574704No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA35540.33576576133988867No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA30940.2923070527815463No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA30520.28833908373926287No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA29090.27482909390482163No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG27920.2637754658584607No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA27380.2586737913755248No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT26150.2470533106088376No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC24860.2348659771218242No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC22960.21691564097816105No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA22530.2128531964824899No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT20090.18980118585589092No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20000.1889509067754016No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA19610.18526636409328126No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT18200.17194532516561545No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG17450.1648596661615379No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17310.1635370098141101No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA17060.1611751234794176No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC16630.15711267898374645No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA16570.15654582626342023No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA16110.15219995540758602No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC15780.14908226544579187No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC15490.14634247729754857No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA15180.14341373824252984No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT15140.14303583642897902No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC14990.1416187046281635No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA14770.1395402446536341No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA14670.13859549011975708No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA14650.13840653921298168No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA14560.13755626013249236No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT14550.1374617846791047No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG14530.1372728337723293No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT14410.13613912833167688No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA13950.13179325747584264No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC13940.1316987820224549No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT13920.13150983111567952No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA13500.1275418620733961No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT13420.12678605844629448No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA13060.12338494212433726No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC12720.12017277670915542No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC12680.11979487489560461No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC12670.11970039944221693No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC12490.1179998412812383No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG11920.11261474043813936No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA11800.11148103499748696No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA11740.11091418227716075No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC11390.10760754140859122No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC11380.10751306595520353No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG11330.10704068868826501No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC11310.10685173778148961No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG11170.10552908143406181No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT11170.10552908143406181No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT11120.10505670416712329No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC11110.1049622287137356No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG10990.10382852327308319No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA10910.10307271964598158No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC10870.10269481783243077No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC10710.10118321057822756No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA10660.10071083331128906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCACT308.311366E-637.034
TTAAGAC352.3881315E-531.71428529
TAAGACT352.3881315E-531.71428530
ATTGACT950.031.15789631
AAGACTC303.5992882E-430.83333231
AGTTAAG303.5992882E-430.83333227
GACTCGG303.5992882E-430.83333233
TAGGATC250.00549614929.631
TATTGAC1000.027.75000230
GTTAAGA405.939694E-527.7528
CAACTTA405.939694E-527.7514
AAATAAA358.8689313E-426.4285737
ACTCGGT358.8689313E-426.4285734
GGTATCA47500.025.5105251
ATACGGC1200.024.66666629
CAATGCT851.9754225E-923.94117533
GTGTATC551.9024745E-523.5454541
TCAATGC802.72521E-823.12532
ACATGGT400.001931087123.1253
GCAACTT502.7019062E-422.213
Produced by FastQC (version 0.11.5)