##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631782.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1058476
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.582524308534154	34.0	31.0	34.0	31.0	34.0
2	32.72080897441227	34.0	31.0	34.0	31.0	34.0
3	32.780183962602834	34.0	31.0	34.0	31.0	34.0
4	36.22542221080119	37.0	35.0	37.0	35.0	37.0
5	36.168868259648775	37.0	35.0	37.0	35.0	37.0
6	36.25179408885983	37.0	37.0	37.0	35.0	37.0
7	36.23909469841546	37.0	37.0	37.0	35.0	37.0
8	36.234614672415816	37.0	37.0	37.0	35.0	37.0
9	38.0462854141237	39.0	38.0	39.0	35.0	39.0
10	37.99030587372789	39.0	38.0	39.0	35.0	39.0
11	38.06655795691164	39.0	38.0	39.0	37.0	39.0
12	37.99686058068393	39.0	38.0	39.0	35.0	39.0
13	38.06171042139831	39.0	38.0	39.0	35.0	39.0
14	39.35793915024998	40.0	39.0	41.0	36.0	41.0
15	39.377331181812345	40.0	39.0	41.0	37.0	41.0
16	39.29927934124156	40.0	39.0	41.0	36.0	41.0
17	39.33153704004626	40.0	39.0	41.0	36.0	41.0
18	39.39646907440509	40.0	39.0	41.0	37.0	41.0
19	39.41914885174534	41.0	39.0	41.0	37.0	41.0
20	39.417278237768265	41.0	39.0	41.0	37.0	41.0
21	39.36160290833236	40.0	39.0	41.0	36.0	41.0
22	39.29959677876494	40.0	39.0	41.0	36.0	41.0
23	39.218824989891125	40.0	39.0	41.0	35.0	41.0
24	39.20769956050019	40.0	39.0	41.0	35.0	41.0
25	39.14260030458886	40.0	39.0	41.0	35.0	41.0
26	39.01000022674109	40.0	38.0	41.0	35.0	41.0
27	38.83571568934959	40.0	38.0	41.0	35.0	41.0
28	38.78479814374629	40.0	38.0	41.0	35.0	41.0
29	38.69373892275309	40.0	38.0	41.0	35.0	41.0
30	38.5388728700509	40.0	38.0	41.0	35.0	41.0
31	38.32957384012486	40.0	37.0	41.0	35.0	41.0
32	38.171082764276186	40.0	37.0	41.0	35.0	41.0
33	38.003876327852495	40.0	37.0	41.0	34.0	41.0
34	37.8886077719287	40.0	37.0	41.0	34.0	41.0
35	37.724881811207815	40.0	36.0	41.0	34.0	41.0
36	37.57556808090122	40.0	36.0	41.0	33.0	41.0
37	37.433066030783884	40.0	35.0	41.0	33.0	41.0
38	37.31137503353879	40.0	35.0	41.0	33.0	41.0
39	37.1102320695037	40.0	35.0	41.0	33.0	41.0
40	36.674705897913604	40.0	35.0	41.0	31.0	41.0
41	36.558111851378776	39.0	35.0	41.0	31.0	41.0
42	36.36482168702928	39.0	35.0	41.0	31.0	41.0
43	35.78604805399461	39.0	35.0	41.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	4.0
11	5.0
12	5.0
13	5.0
14	3.0
15	3.0
16	3.0
17	9.0
18	17.0
19	30.0
20	91.0
21	156.0
22	305.0
23	512.0
24	1023.0
25	1660.0
26	2700.0
27	4357.0
28	6592.0
29	9406.0
30	12488.0
31	15257.0
32	18959.0
33	23778.0
34	32992.0
35	46926.0
36	69000.0
37	144782.0
38	236222.0
39	431181.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.63292129438929	17.17582637679078	14.42441774778077	24.766834581039156
2	20.21037793960373	22.260778704477	32.67310737324228	24.85573598267698
3	21.910180296955247	22.37103155858045	30.328793472879877	25.389994671584432
4	14.845872745343305	19.131940639183128	34.04460753007154	31.97757908540203
5	16.133951076831217	33.61975141618704	32.77183422203243	17.474463284949305
6	28.44334684962153	34.848026785680545	17.483155026660974	19.22547133803695
7	26.886580328699	30.05179144354714	21.5371912069806	21.524437020773263
8	27.081294238131047	30.410987117327178	21.067648203643728	21.440070440898047
9	28.633148035477422	13.347019677347433	19.84759219859496	38.17224008858018
10	20.85158284174606	24.463946277478186	28.031055971037606	26.653414909738153
11	35.73562367025799	21.751556010717294	19.349423132881615	23.163397186143097
12	21.437708554563354	26.862016710818192	27.890098594583158	23.810176140035296
13	35.97955929090504	18.46862848094808	22.645766177031884	22.906046051115002
14	22.750161553025293	23.447295923573137	27.59117826006447	26.2113642633371
15	29.717820715821613	24.932922428094734	23.20137631840495	22.1478805376787
16	21.055555345610102	25.461134687985364	27.465998284325764	26.017311682078763
17	23.793076082972124	25.466897690642014	25.398025085122384	25.342001141263477
18	24.622287137355972	21.04506857028407	27.556694719577955	26.775949572781997
19	25.454710357155	23.76964617053197	29.403406406947347	21.372237065365677
20	27.444268930046594	21.230240458923962	28.46006900487115	22.865421606158286
21	24.501264081566326	23.427550553815106	28.02104157297851	24.050143791640053
22	24.755025149365693	24.227285266741998	27.818675151822053	23.199014432070257
23	24.533480211171533	23.140534126423272	28.7800573654953	23.545928296909896
24	23.965871687218225	23.097642270585254	29.722544488491	23.213941553705517
25	23.66487289272501	23.94272520113824	29.401989275146533	22.990412630990217
26	23.77002407234552	24.942464448886888	28.526201822242548	22.761309656525043
27	23.473371148708143	23.52656082896542	30.83659903483877	22.163468987487672
28	22.77822076268144	25.057630026566496	27.71749194124383	24.446657269508236
29	23.219799031815555	25.50988402193342	28.283777808849702	22.986539137401323
30	23.038689587671332	23.6119666388279	30.14900668508308	23.20033708841769
31	24.020950876543257	24.043247083542756	28.679535483090785	23.256266556823206
32	21.712159746654624	24.41614169806401	28.862723387209538	25.008975168071835
33	21.757224537920557	23.79439873931955	30.50772998159618	23.94064674116371
34	22.049531590702102	23.98004300522638	29.475491177882162	24.49493422618935
35	21.55977084034026	25.365147627343465	30.0213703475563	23.053711184759976
36	22.140322501407685	24.046648199864713	30.145133191494182	23.667896107233418
37	22.518791167678813	24.129975549752665	29.34294211677922	24.008291165789306
38	21.848582301346465	23.256266556823206	30.000113370544064	24.895037771286262
39	21.872484591053553	22.173861287360317	31.23405726724083	24.719596854345305
40	21.428544435584744	23.808570057327707	30.922477221968187	23.84040828511936
41	19.32826063132277	23.20345477837948	31.932514294136098	25.53577029616165
42	17.86379662836002	24.071684195012452	32.07621145873879	25.988307717888738
43	17.379420978841278	25.590282632766353	30.868437262630422	26.161859125761943
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	17.0
1	62.0
2	107.0
3	404.5
4	702.0
5	702.0
6	1046.5
7	1391.0
8	1672.0
9	1953.0
10	3115.5
11	4278.0
12	4278.0
13	8337.0
14	12396.0
15	20870.0
16	29344.0
17	27993.0
18	26642.0
19	26642.0
20	28815.5
21	30989.0
22	22587.0
23	14185.0
24	11670.0
25	9155.0
26	9155.0
27	7859.0
28	6563.0
29	5867.5
30	5172.0
31	4923.0
32	4674.0
33	4674.0
34	5976.5
35	7279.0
36	7069.5
37	6860.0
38	10484.5
39	14109.0
40	14109.0
41	20814.5
42	27520.0
43	36303.5
44	45087.0
45	70516.0
46	95945.0
47	95945.0
48	108982.0
49	122019.0
50	135708.0
51	149397.0
52	140041.5
53	130686.0
54	130686.0
55	110884.0
56	91082.0
57	80882.0
58	70682.0
59	62088.5
60	53495.0
61	53495.0
62	46726.0
63	39957.0
64	30331.5
65	20706.0
66	17469.5
67	14233.0
68	14233.0
69	11895.5
70	9558.0
71	7529.5
72	5501.0
73	4185.0
74	2869.0
75	2869.0
76	2408.5
77	1948.0
78	1521.5
79	1095.0
80	814.0
81	533.0
82	533.0
83	389.0
84	245.0
85	160.0
86	75.0
87	47.5
88	20.0
89	20.0
90	13.5
91	7.0
92	3.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1058476.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.338179369149426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.97220060875502	17.963910242452773
2	9.119382960758932	4.256595905483137
3	3.567568025920151	2.497816275017004
4	2.0720631596327053	1.9343272673485832
5	1.2666454250519594	1.4780599063487572
6	0.9321466487208545	1.3052763411719293
7	0.6752315263818252	1.1031072134882514
8	0.5402428235340797	1.0086627134827275
9	0.45128946753643606	0.9479047086695953
>10	3.3729394219903917	16.180358953848177
>50	0.4959596939014943	8.103405330113747
>100	0.4518743875191313	20.89084015088772
>500	0.05673942173907792	8.895064233337303
>1k	0.024491836721904137	9.496953640393295
>5k	0.0	0.0
>10k+	0.0012245918360952069	3.937717117957011
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14922	1.4097627154512715	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14809	1.3990869892184612	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11603	1.0961986856574926	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3907	0.36911559638574704	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	3554	0.33576576133988867	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	3094	0.2923070527815463	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	3052	0.28833908373926287	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2909	0.27482909390482163	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	2792	0.2637754658584607	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	2738	0.2586737913755248	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	2615	0.2470533106088376	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	2486	0.2348659771218242	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	2296	0.21691564097816105	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2253	0.2128531964824899	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	2009	0.18980118585589092	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2000	0.1889509067754016	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1961	0.18526636409328126	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1820	0.17194532516561545	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1745	0.1648596661615379	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1731	0.1635370098141101	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1706	0.1611751234794176	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	1663	0.15711267898374645	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1657	0.15654582626342023	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	1611	0.15219995540758602	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	1578	0.14908226544579187	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1549	0.14634247729754857	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	1518	0.14341373824252984	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	1514	0.14303583642897902	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	1499	0.1416187046281635	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	1477	0.1395402446536341	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1467	0.13859549011975708	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	1465	0.13840653921298168	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1456	0.13755626013249236	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	1455	0.1374617846791047	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	1453	0.1372728337723293	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	1441	0.13613912833167688	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	1395	0.13179325747584264	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	1394	0.1316987820224549	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	1392	0.13150983111567952	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	1350	0.1275418620733961	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1342	0.12678605844629448	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	1306	0.12338494212433726	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	1272	0.12017277670915542	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	1268	0.11979487489560461	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	1267	0.11970039944221693	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	1249	0.1179998412812383	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	1192	0.11261474043813936	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	1180	0.11148103499748696	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	1174	0.11091418227716075	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	1139	0.10760754140859122	No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC	1138	0.10751306595520353	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	1133	0.10704068868826501	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	1131	0.10685173778148961	No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG	1117	0.10552908143406181	No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT	1117	0.10552908143406181	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	1112	0.10505670416712329	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	1111	0.1049622287137356	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	1099	0.10382852327308319	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	1091	0.10307271964598158	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	1087	0.10269481783243077	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	1071	0.10118321057822756	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	1066	0.10071083331128906	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	9.44754533877008E-5	0.0	0.0	0.0	0.0
3	9.44754533877008E-5	0.0	0.0	0.0	0.0
4	9.44754533877008E-5	0.0	0.0	0.0	0.0
5	9.44754533877008E-5	0.0	0.0	0.0	0.0
6	9.44754533877008E-5	0.0	0.0	0.0	9.44754533877008E-5
7	9.44754533877008E-5	0.0	0.0	0.0	9.44754533877008E-5
8	9.44754533877008E-5	0.0	0.0	0.0	9.44754533877008E-5
9	9.44754533877008E-5	0.0	0.0	9.44754533877008E-5	9.44754533877008E-5
10	9.44754533877008E-5	0.0	0.0	1.889509067754016E-4	9.44754533877008E-5
11	9.44754533877008E-5	0.0	0.0	0.0010392299872647088	9.44754533877008E-5
12	9.44754533877008E-5	0.0	0.0	0.0017005581609786146	9.44754533877008E-5
13	1.889509067754016E-4	0.0	0.0	0.0021729354279171184	9.44754533877008E-5
14	1.889509067754016E-4	0.0	0.0	0.0030232145084064257	9.44754533877008E-5
15	1.889509067754016E-4	0.0	0.0	0.003873493588895733	9.44754533877008E-5
16	1.889509067754016E-4	0.0	0.0	0.005385100843098946	9.44754533877008E-5
17	1.889509067754016E-4	0.0	0.0	0.007746987177791466	9.44754533877008E-5
18	2.8342636016310245E-4	0.0	0.0	0.009636496245545483	9.44754533877008E-5
19	2.8342636016310245E-4	0.0	0.0	0.011903907126850301	9.44754533877008E-5
20	2.8342636016310245E-4	0.0	0.0	0.015871876169133735	9.44754533877008E-5
21	2.8342636016310245E-4	0.0	0.0	0.02210725609272199	9.44754533877008E-5
22	3.779018135508032E-4	0.0	0.0	0.028815013283248748	9.44754533877008E-5
23	3.779018135508032E-4	0.0	0.0	0.03420011412634769	9.44754533877008E-5
24	3.779018135508032E-4	0.0	0.0	0.04553716853287179	9.44754533877008E-5
25	3.779018135508032E-4	0.0	0.0	0.05252835208356165	9.44754533877008E-5
26	3.779018135508032E-4	0.0	0.0	0.06651071918494136	9.44754533877008E-5
27	3.779018135508032E-4	0.0	0.0	0.13680045650539077	9.44754533877008E-5
28	3.779018135508032E-4	0.0	0.0	0.38309796348712677	9.44754533877008E-5
29	3.779018135508032E-4	0.0	0.0	0.7366251100639032	9.44754533877008E-5
30	3.779018135508032E-4	0.0	0.0	1.1600640921475782	9.44754533877008E-5
31	3.779018135508032E-4	0.0	0.0	2.192680797675148	9.44754533877008E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCACT	30	8.311366E-6	37.0	34
TTAAGAC	35	2.3881315E-5	31.714285	29
TAAGACT	35	2.3881315E-5	31.714285	30
ATTGACT	95	0.0	31.157896	31
AAGACTC	30	3.5992882E-4	30.833332	31
AGTTAAG	30	3.5992882E-4	30.833332	27
GACTCGG	30	3.5992882E-4	30.833332	33
TAGGATC	25	0.005496149	29.6	31
TATTGAC	100	0.0	27.750002	30
GTTAAGA	40	5.939694E-5	27.75	28
CAACTTA	40	5.939694E-5	27.75	14
AAATAAA	35	8.8689313E-4	26.42857	37
ACTCGGT	35	8.8689313E-4	26.42857	34
GGTATCA	4750	0.0	25.510525	1
ATACGGC	120	0.0	24.666666	29
CAATGCT	85	1.9754225E-9	23.941175	33
GTGTATC	55	1.9024745E-5	23.545454	1
TCAATGC	80	2.72521E-8	23.125	32
ACATGGT	40	0.0019310871	23.125	3
GCAACTT	50	2.7019062E-4	22.2	13
>>END_MODULE