FastQCFastQC Report
Fri 10 Feb 2017
ERR1631781.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631781.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1480253
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT85230.5757799511299758No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT81550.5509193360864663No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT63370.42810249328999844No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22680.15321705140945502No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA19730.13328802576316345No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA19390.13099112111240444No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA17240.1164665769973106No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16630.11234565982977235No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA16430.11099453944697292No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG15120.10214470093963667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28700.028.233451
TATACAC6700.019.32835837
GTAAAAC1153.0522642E-919.3043483
TAATAAG609.238526E-418.52
GTATCAA43750.018.4788572
TATTAGA2250.018.0888882
GTATTAG3350.017.1194041
TCCTATA650.001580224717.0769232
CTTATAC19600.016.89540937
ATACACA3350.016.56716337
CAGTCGG3500.016.38571410
ATTAGAG2350.015.7446793
GCAGTCG3600.015.4166659
AGTCGGT3750.015.29333411
CTCTATG6200.015.2177421
CGAACTA2201.8189894E-1215.13636424
GCTAGGC1101.4523504E-515.1363643
TCTTATA32650.015.12863637
TCTCGTC1353.9740917E-715.07407512
TAATACT2109.094947E-1214.97619154
Produced by FastQC (version 0.11.5)