##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631781.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1480253
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.46949474177725	33.0	31.0	34.0	31.0	34.0
2	32.63512588726387	34.0	31.0	34.0	31.0	34.0
3	32.721152397596896	34.0	31.0	34.0	31.0	34.0
4	36.20342806263524	37.0	35.0	37.0	35.0	37.0
5	36.10072872677846	37.0	35.0	37.0	35.0	37.0
6	36.15107485004253	37.0	36.0	37.0	35.0	37.0
7	36.139745705632755	37.0	36.0	37.0	35.0	37.0
8	36.12556164385413	37.0	36.0	37.0	35.0	37.0
9	37.918778749308395	39.0	38.0	39.0	35.0	39.0
10	37.843174781608276	39.0	38.0	39.0	35.0	39.0
11	37.913979569708694	39.0	38.0	39.0	35.0	39.0
12	37.859998257054706	39.0	38.0	39.0	35.0	39.0
13	37.9115124238897	39.0	38.0	39.0	35.0	39.0
14	39.25690608294663	40.0	39.0	41.0	36.0	41.0
15	39.248827058617685	40.0	39.0	41.0	36.0	41.0
16	39.19786279777849	40.0	39.0	41.0	36.0	41.0
17	39.18159328168901	40.0	39.0	41.0	36.0	41.0
18	39.21313451146527	40.0	39.0	41.0	36.0	41.0
19	39.25128677327457	40.0	39.0	41.0	36.0	41.0
20	39.233701941492434	40.0	39.0	41.0	36.0	41.0
21	39.20203100415942	40.0	39.0	41.0	36.0	41.0
22	39.16742543335497	40.0	39.0	41.0	36.0	41.0
23	39.10341542965966	40.0	39.0	41.0	36.0	41.0
24	39.11358058385965	40.0	39.0	41.0	36.0	41.0
25	39.050019152131426	40.0	39.0	41.0	35.0	41.0
26	38.97156161818284	40.0	38.0	41.0	35.0	41.0
27	38.86204723111522	40.0	38.0	41.0	35.0	41.0
28	38.836538078287965	40.0	38.0	41.0	35.0	41.0
29	38.78944342622511	40.0	38.0	41.0	35.0	41.0
30	38.71116896908839	40.0	38.0	41.0	35.0	41.0
31	38.59538943680573	40.0	38.0	41.0	35.0	41.0
32	38.511118707410155	40.0	38.0	41.0	35.0	41.0
33	38.424736852416444	40.0	38.0	41.0	35.0	41.0
34	38.36201581756632	40.0	38.0	41.0	34.0	41.0
35	38.26322054405564	40.0	38.0	41.0	34.0	41.0
36	38.19290351041342	40.0	38.0	41.0	34.0	41.0
37	38.12308774243322	40.0	38.0	41.0	34.0	41.0
38	38.05759691079836	40.0	37.0	41.0	34.0	41.0
39	37.96425475915266	40.0	37.0	41.0	34.0	41.0
40	37.63094687191987	40.0	37.0	41.0	33.0	41.0
41	37.655666294883375	40.0	37.0	41.0	33.0	41.0
42	37.58396030948764	40.0	37.0	41.0	33.0	41.0
43	37.08215419931593	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	1.0
13	0.0
14	2.0
15	6.0
16	5.0
17	5.0
18	15.0
19	38.0
20	91.0
21	176.0
22	342.0
23	585.0
24	987.0
25	1776.0
26	2916.0
27	4452.0
28	6772.0
29	9636.0
30	13376.0
31	17751.0
32	23103.0
33	30773.0
34	43137.0
35	62892.0
36	96850.0
37	180783.0
38	377068.0
39	606712.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.25676286418605	18.331190681592943	13.651585235767131	25.760461218453873
2	19.18881434457488	21.26116278771264	34.69521764184906	24.854805225863416
3	20.599316468198342	22.34037019347368	30.322451634956998	26.73786170337098
4	15.146262159238995	17.206788299027263	34.585641778804025	33.061307762929715
5	15.786287884571085	34.576454160200996	33.675054196816355	15.962203758411569
6	31.936027152115216	35.05448055163543	16.376524823797013	16.632967472452346
7	28.191126787110043	29.75241394545392	21.551856338071936	20.5046029293641
8	25.98008583667792	32.99294107156007	20.69484067926226	20.332132412499753
9	27.050105623835925	14.588316997161971	19.87004924158235	38.49152813741975
10	18.65437867715857	25.67547574637579	31.28154443868717	24.388601137778473
11	35.64823040385664	21.696291106993197	21.015191322024005	21.64028716712616
12	21.59786198710626	25.880035372331623	28.371028466079785	24.151074174482336
13	32.13214227567855	19.51848771797794	23.702637319431204	24.646732686912305
14	22.759285068160644	21.812960352047927	25.830314142244603	29.597440437546823
15	27.199742206230965	26.276859428759813	22.942294324010827	23.581104040998397
16	23.622178100635498	26.054397457731888	25.01254853055525	25.310875911077364
17	24.249908630484114	25.840650213173017	24.99613241790424	24.913308738438634
18	23.854638362496143	23.866528221864776	26.94586668630295	25.332966729336132
19	25.555698924440616	24.5147282255128	27.178124280106168	22.751448569940408
20	26.158906619341423	23.41370022556955	27.0054510951844	23.421942059904623
21	24.40062610918539	24.997686206344454	25.932593955222522	24.669093729247635
22	24.72867813812909	25.134419589083755	25.757758977688272	24.379143295098878
23	24.528104317302514	24.795693709115945	26.582685527406465	24.09351644617508
24	25.091724184987296	24.56647613617402	26.3163796999567	24.025419978881988
25	24.7986661739581	24.439335708152594	26.839533512176633	23.922464605712673
26	24.546479554508586	25.13367647287322	26.370559627306957	23.94928434531124
27	24.485003577091213	24.376508610352417	26.964444591566444	24.174043220989923
28	23.605424207888788	25.25534486334431	26.351171049813782	24.788059878953124
29	24.338339459538336	25.73992418863532	26.2139647749405	23.707771576885843
30	23.87841808123341	25.060986196278613	27.137523112603045	23.923072609884933
31	24.596335896633885	24.749417836005062	26.652673563235474	24.00157270412558
32	22.65410034635971	24.949856544793356	26.905062850742407	25.49098025810453
33	23.270751689069368	24.459467401856305	27.772347024461357	24.497433884612967
34	23.472676630278745	24.443997073473252	27.361673984109476	24.721652312138534
35	23.43207546277562	25.145431220203573	27.251490116892178	24.171003200128627
36	23.470649949704544	24.269297207977285	27.86239919797494	24.39765364434323
37	23.773368471470754	23.60238418702749	27.517795944341948	25.106451397159812
38	23.069434752032254	23.349319339329156	28.598759806600626	24.982486102037964
39	22.63315798042632	22.861497325119423	29.393353703724973	25.11199099072929
40	22.541822242549078	23.466934368651845	29.61365388214042	24.37758950665866
41	21.185500046275873	23.236095451250563	30.387237857312225	25.191166645161335
42	20.547771225594545	23.578401800232797	30.274419305348477	25.59940766882418
43	19.96645168089509	24.13236115718056	30.101543452369288	25.799643709555053
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	355.0
1	289.5
2	224.0
3	475.0
4	726.0
5	726.0
6	901.5
7	1077.0
8	1162.0
9	1247.0
10	1971.5
11	2696.0
12	2696.0
13	5051.0
14	7406.0
15	12700.0
16	17994.0
17	17339.0
18	16684.0
19	16684.0
20	18522.0
21	20360.0
22	17632.5
23	14905.0
24	15010.0
25	15115.0
26	15115.0
27	15811.5
28	16508.0
29	20776.5
30	25045.0
31	27382.0
32	29719.0
33	29719.0
34	34029.0
35	38339.0
36	41243.0
37	44147.0
38	52330.5
39	60514.0
40	60514.0
41	68059.5
42	75605.0
43	80065.5
44	84526.0
45	98236.0
46	111946.0
47	111946.0
48	120204.0
49	128462.0
50	139754.5
51	151047.0
52	149620.0
53	148193.0
54	148193.0
55	129779.5
56	111366.0
57	101263.0
58	91160.0
59	81951.5
60	72743.0
61	72743.0
62	65850.5
63	58958.0
64	49408.0
65	39858.0
66	34800.0
67	29742.0
68	29742.0
69	25577.5
70	21413.0
71	18409.0
72	15405.0
73	12812.5
74	10220.0
75	10220.0
76	8295.0
77	6370.0
78	5235.0
79	4100.0
80	3315.0
81	2530.0
82	2530.0
83	2025.0
84	1520.0
85	1281.0
86	1042.0
87	807.0
88	572.0
89	572.0
90	435.0
91	298.0
92	198.0
93	98.0
94	57.5
95	17.0
96	17.0
97	9.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1480253.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.96619730758333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.50273398581906	37.592300727583314
2	7.049519566824023	6.198811363973087
3	2.2227265782000454	2.931745058938582
4	1.1694142347828826	2.0565878792304315
5	0.7085474616469621	1.5576068750278833
6	0.4942829126559984	1.3039044038160361
7	0.3503716335176282	1.078315585915142
8	0.2599626443765806	0.9143655132209488
9	0.20431873258726535	0.8084805939510357
>10	1.5891679297050958	14.068832482010144
>50	0.23435209851893618	7.218725346504054
>100	0.19651255882223007	16.513033795180654
>500	0.014533575034827564	4.290723197867345
>1k	0.00309225000741012	1.9020710677998443
>5k	4.63837501111518E-4	1.5644961089815737
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8523	0.5757799511299758	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8155	0.5509193360864663	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6337	0.42810249328999844	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2268	0.15321705140945502	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1973	0.13328802576316345	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1939	0.13099112111240444	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1724	0.1164665769973106	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1663	0.11234565982977235	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1643	0.11099453944697292	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1512	0.10214470093963667	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	6.755601913997135E-5	0.0	0.0	0.0
2	0.0	6.755601913997135E-5	0.0	0.0	0.0
3	0.0	6.755601913997135E-5	0.0	6.755601913997135E-5	0.0
4	0.0	6.755601913997135E-5	0.0	6.755601913997135E-5	0.0
5	0.0	6.755601913997135E-5	0.0	6.755601913997135E-5	0.0
6	0.0	6.755601913997135E-5	0.0	6.755601913997135E-5	0.0
7	0.0	6.755601913997135E-5	0.0	6.755601913997135E-5	0.0
8	6.755601913997135E-5	6.755601913997135E-5	0.0	6.755601913997135E-5	0.0
9	6.755601913997135E-5	6.755601913997135E-5	0.0	1.351120382799427E-4	0.0
10	6.755601913997135E-5	6.755601913997135E-5	0.0	1.351120382799427E-4	0.0
11	6.755601913997135E-5	6.755601913997135E-5	0.0	2.702240765598854E-4	0.0
12	6.755601913997135E-5	6.755601913997135E-5	0.0	4.728921339797994E-4	0.0
13	6.755601913997135E-5	6.755601913997135E-5	0.0	6.08004172259742E-4	0.0
14	6.755601913997135E-5	6.755601913997135E-5	0.0	7.431162105396848E-4	0.0
15	6.755601913997135E-5	6.755601913997135E-5	0.0	0.0011484523253795127	0.0
16	6.755601913997135E-5	6.755601913997135E-5	0.0	0.0017564564976392549	0.0
17	1.351120382799427E-4	6.755601913997135E-5	0.0	0.002567128727318911	0.0
18	1.351120382799427E-4	6.755601913997135E-5	0.0	0.003175132899578653	0.0
19	1.351120382799427E-4	6.755601913997135E-5	0.0	0.0036480250335584525	0.0
20	1.351120382799427E-4	6.755601913997135E-5	6.755601913997135E-5	0.004391141244098137	0.0
21	1.351120382799427E-4	6.755601913997135E-5	6.755601913997135E-5	0.005134257454637822	0.0
22	1.351120382799427E-4	6.755601913997135E-5	6.755601913997135E-5	0.007904054239376648	0.0
23	1.351120382799427E-4	6.755601913997135E-5	6.755601913997135E-5	0.010268514909275644	0.0
24	1.351120382799427E-4	6.755601913997135E-5	6.755601913997135E-5	0.013240979751434383	0.0
25	1.351120382799427E-4	6.755601913997135E-5	6.755601913997135E-5	0.015267660325633524	0.0
26	1.351120382799427E-4	6.755601913997135E-5	6.755601913997135E-5	0.02107747797167106	0.0
27	2.702240765598854E-4	6.755601913997135E-5	6.755601913997135E-5	0.05816573247951533	0.0
28	2.702240765598854E-4	6.755601913997135E-5	6.755601913997135E-5	0.1805772391611434	0.0
29	3.377800956998567E-4	6.755601913997135E-5	6.755601913997135E-5	0.35088596341301115	0.0
30	3.377800956998567E-4	6.755601913997135E-5	6.755601913997135E-5	0.5648358760293004	0.0
31	3.377800956998567E-4	6.755601913997135E-5	6.755601913997135E-5	1.183784123389718	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2870	0.0	28.23345	1
TATACAC	670	0.0	19.328358	37
GTAAAAC	115	3.0522642E-9	19.304348	3
TAATAAG	60	9.238526E-4	18.5	2
GTATCAA	4375	0.0	18.478857	2
TATTAGA	225	0.0	18.088888	2
GTATTAG	335	0.0	17.119404	1
TCCTATA	65	0.0015802247	17.076923	2
CTTATAC	1960	0.0	16.895409	37
ATACACA	335	0.0	16.567163	37
CAGTCGG	350	0.0	16.385714	10
ATTAGAG	235	0.0	15.744679	3
GCAGTCG	360	0.0	15.416665	9
AGTCGGT	375	0.0	15.293334	11
CTCTATG	620	0.0	15.217742	1
CGAACTA	220	1.8189894E-12	15.136364	24
GCTAGGC	110	1.4523504E-5	15.136364	3
TCTTATA	3265	0.0	15.128636	37
TCTCGTC	135	3.9740917E-7	15.074075	12
TAATACT	210	9.094947E-12	14.9761915	4
>>END_MODULE