FastQCFastQC Report
Fri 10 Feb 2017
ERR1631780.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631780.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665016
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23310.35051788227651665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23020.3461570849423172No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18290.275030976698305No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA13170.1980403479014039No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12100.1819505094614265No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA11170.16796588352761438No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA11110.1670636495964007No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11040.16601104334331807No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT9900.14886859865025803No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG9800.14736487543156856No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA9630.14480854595979645No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC9350.14059812094746593No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC8870.13338024949775645No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT8100.12180158071384747No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA7770.11683929409217221No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7280.10947105032059379No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7260.10917030567685589No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7040.10586211459573905No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAT200.001841377937.05
TATACAG250.005494698429.65
GACCTAG358.865448E-426.4285721
GGTATCA10500.025.0190471
ATACACA1155.456968E-1222.5217437
CTTATAC7250.020.92413737
ATACGGC450.003824137620.55555729
TTCGCTA555.1399623E-420.18181826
TATAACT555.1399623E-420.1818184
CTCTAAT555.1399623E-420.1818181
TGCGGGT3950.020.1392421
ATAAGCC759.256144E-619.7333343
CAGTCGG2800.019.16071510
CTCTATG4950.019.0606061
TTCTGCG4200.018.94047718
TAATACT1002.8704017E-718.54
CGTTATT500.007031840718.510
ACAATTA500.007031840718.535
TACTAGG500.007031840718.52
CTAGGAC701.21821686E-418.53
Produced by FastQC (version 0.11.5)