##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631780.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	665016
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.634423833411525	34.0	31.0	34.0	31.0	34.0
2	32.79898679129525	34.0	31.0	34.0	31.0	34.0
3	32.88594560130884	34.0	31.0	34.0	31.0	34.0
4	36.310049683015144	37.0	37.0	37.0	35.0	37.0
5	36.22975086313713	37.0	35.0	37.0	35.0	37.0
6	36.28513148555824	37.0	37.0	37.0	35.0	37.0
7	36.27081904796275	37.0	37.0	37.0	35.0	37.0
8	36.253556305412204	37.0	37.0	37.0	35.0	37.0
9	38.08095594692458	39.0	38.0	39.0	37.0	39.0
10	38.02174383774225	39.0	38.0	39.0	35.0	39.0
11	38.08953168044077	39.0	38.0	39.0	37.0	39.0
12	38.03277364755134	39.0	38.0	39.0	35.0	39.0
13	38.0822686972945	39.0	38.0	39.0	37.0	39.0
14	39.47644116833279	40.0	39.0	41.0	37.0	41.0
15	39.48113729594476	41.0	39.0	41.0	37.0	41.0
16	39.431093387226774	40.0	39.0	41.0	37.0	41.0
17	39.40453312401506	40.0	39.0	41.0	37.0	41.0
18	39.43357603426083	41.0	39.0	41.0	37.0	41.0
19	39.46142348454774	41.0	39.0	41.0	37.0	41.0
20	39.453498863185246	41.0	39.0	41.0	37.0	41.0
21	39.42160038254719	41.0	39.0	41.0	37.0	41.0
22	39.39441757792294	40.0	39.0	41.0	37.0	41.0
23	39.33429120502364	40.0	39.0	41.0	36.0	41.0
24	39.345379660038255	41.0	39.0	41.0	36.0	41.0
25	39.28826674846921	40.0	39.0	41.0	36.0	41.0
26	39.22304576130499	40.0	39.0	41.0	36.0	41.0
27	39.11892044702684	40.0	39.0	41.0	35.0	41.0
28	39.102871209113765	40.0	39.0	41.0	35.0	41.0
29	39.07283133037401	40.0	39.0	41.0	35.0	41.0
30	39.01606577886848	40.0	39.0	41.0	35.0	41.0
31	38.91469077435731	40.0	38.0	41.0	35.0	41.0
32	38.8562771422041	40.0	38.0	41.0	35.0	41.0
33	38.795636495964004	40.0	38.0	41.0	35.0	41.0
34	38.75488860418396	40.0	38.0	41.0	35.0	41.0
35	38.66932976048697	40.0	38.0	41.0	35.0	41.0
36	38.6131882541172	40.0	38.0	41.0	35.0	41.0
37	38.549616851323876	40.0	38.0	41.0	35.0	41.0
38	38.506305111455966	40.0	38.0	41.0	35.0	41.0
39	38.41742755061532	40.0	38.0	41.0	35.0	41.0
40	38.10317646492716	40.0	38.0	41.0	34.0	41.0
41	38.147211495663264	40.0	37.0	41.0	34.0	41.0
42	38.107786880315665	40.0	37.0	41.0	34.0	41.0
43	37.62786158528517	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	0.0
14	0.0
15	0.0
16	1.0
17	7.0
18	4.0
19	13.0
20	24.0
21	41.0
22	100.0
23	181.0
24	304.0
25	541.0
26	866.0
27	1450.0
28	2204.0
29	3347.0
30	4662.0
31	6234.0
32	8348.0
33	11198.0
34	16762.0
35	24563.0
36	38034.0
37	70251.0
38	153687.0
39	322192.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.49927821285503	17.27311824076413	13.410654781238346	26.816948765142495
2	19.764787614132594	20.21319787794579	34.22699604220048	25.795018465721125
3	20.924910077351523	21.121597074356107	29.86499572942606	28.08849711886631
4	15.275121200091426	16.831775476078768	34.3703008649416	33.52280245888821
5	16.388477871209115	33.49799102577983	33.66054350572016	16.452987597290893
6	32.40222791632081	34.34097826217715	16.29088623431617	16.96590758718587
7	29.114637843300013	28.791788468247383	20.957390498875213	21.13618318957739
8	26.390643232644027	31.874571438882676	20.632435911316417	21.10234941715688
9	27.78970731531271	13.894853657656355	18.919845537550977	39.39559348947995
10	19.06495482815451	24.511891443213397	30.45776342223345	25.965390306398646
11	36.88422534194666	20.906263909439772	20.096358583853622	22.113152164759946
12	21.913307348996113	24.97563968385723	27.73015987585261	25.380893091294045
13	33.479795974833685	18.72932380574302	22.47975988547644	25.311120333946853
14	23.366956584503228	21.23693264522959	24.75549460464109	30.64061616562609
15	28.29646204001107	25.269166456145413	21.671508655430845	24.76286284841267
16	24.285280354156892	25.252926245383573	24.041677192729196	26.42011620773034
17	25.270670179364107	25.004661541977935	23.634920062073693	26.089748216584262
18	25.11849338963273	22.777647455098823	25.362246923382294	26.74161223188615
19	26.6900345254851	23.551914540402034	25.578031205264235	24.180019728848627
20	27.82489443863005	22.196308058753473	25.16255247994033	24.816245022676146
21	25.861182287343464	23.771157385686962	24.126186437619545	26.241473889350033
22	26.30102132881013	24.150997870727924	23.81852466707568	25.729456133386265
23	26.101477257690043	23.45311992493414	24.83444007362229	25.610962743753536
24	26.235458996475273	23.297634944121643	24.894438630048	25.572467429355083
25	26.144934858710165	23.445902053484428	25.186311306795627	25.22285178100978
26	26.002081152934664	24.2198683941439	24.531289472734493	25.246760980186945
27	25.70434395563415	23.178991182167046	25.78283830764974	25.333826554549066
28	24.81007975747952	24.114908513479374	24.51414702804143	26.560864700999677
29	25.566452536480327	24.62151286585586	24.583919785388623	25.228114812275194
30	25.235332683724902	23.71582037123919	25.745846716469977	25.30300022856593
31	26.086590397825017	23.71145957390499	24.833988956656682	25.367961071613315
32	24.094457877705196	23.71597074356106	25.2571366703959	26.932434708337844
33	24.35294789899792	23.366655839859494	26.47304726502821	25.807348996114378
34	24.85865001744319	23.277635425313076	25.809303836298675	26.05441072094506
35	24.452043259109555	24.395202521443093	26.112003320220868	25.04075089922648
36	24.6837670071096	23.380790838115175	26.42733407918005	25.508108075595175
37	25.299240920519207	22.850578031205266	26.064786411154017	25.785394637121513
38	24.507831390522934	22.415250159394663	26.94536672801857	26.13155172206383
39	24.005888580124388	21.863684482779362	27.914365970142075	26.21606096695418
40	23.771007013365093	22.605771891202618	28.216915081742393	25.406306013689893
41	22.2068341212843	22.142925884489998	29.23012378649536	26.42011620773034
42	21.40775560287271	22.64531981185415	29.435682750490216	26.511241834782922
43	20.82656657884923	23.269064202966543	29.157042838067053	26.74732638011717
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	25.0
1	29.0
2	33.0
3	85.5
4	138.0
5	138.0
6	178.0
7	218.0
8	241.0
9	264.0
10	401.0
11	538.0
12	538.0
13	1092.5
14	1647.0
15	3039.5
16	4432.0
17	4350.0
18	4268.0
19	4268.0
20	4746.5
21	5225.0
22	4457.5
23	3690.0
24	3838.0
25	3986.0
26	3986.0
27	4319.5
28	4653.0
29	5905.0
30	7157.0
31	8208.0
32	9259.0
33	9259.0
34	10890.5
35	12522.0
36	13824.0
37	15126.0
38	18470.0
39	21814.0
40	21814.0
41	25241.0
42	28668.0
43	31640.0
44	34612.0
45	43639.5
46	52667.0
47	52667.0
48	57278.0
49	61889.0
50	69113.0
51	76337.0
52	75423.5
53	74510.0
54	74510.0
55	66194.5
56	57879.0
57	52841.0
58	47803.0
59	43114.0
60	38425.0
61	38425.0
62	34820.5
63	31216.0
64	25576.5
65	19937.0
66	17353.5
67	14770.0
68	14770.0
69	12742.5
70	10715.0
71	9277.0
72	7839.0
73	6363.0
74	4887.0
75	4887.0
76	4009.0
77	3131.0
78	2575.5
79	2020.0
80	1615.0
81	1210.0
82	1210.0
83	951.5
84	693.0
85	557.5
86	422.0
87	318.0
88	214.0
89	214.0
90	173.0
91	132.0
92	84.5
93	37.0
94	22.5
95	8.0
96	8.0
97	4.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	665016.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.794549492977104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.54217497793226	40.029075407417594
2	6.7537481569988165	6.320772047915681
3	2.2529580436258976	3.1627847003415885
4	1.163468868485164	2.1777600619946833
5	0.7147510396145181	1.6723226449199207
6	0.5197800419891665	1.4593723740194262
7	0.3571190110733693	1.1697856266989073
8	0.29371215781286203	1.0995302484370462
9	0.2222129149499079	0.935851792194196
>10	1.7963186114279959	17.29302445521969
>50	0.2287652766493311	7.520883545329586
>100	0.14366588591166765	12.524206790752698
>500	0.008736439008141944	2.769058741332107
>1k	0.0025885745209309466	1.8655715634269951
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2331	0.35051788227651665	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2302	0.3461570849423172	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1829	0.275030976698305	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1317	0.1980403479014039	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1210	0.1819505094614265	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1117	0.16796588352761438	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1111	0.1670636495964007	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1104	0.16601104334331807	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	990	0.14886859865025803	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	980	0.14736487543156856	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	963	0.14480854595979645	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	935	0.14059812094746593	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	887	0.13338024949775645	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	810	0.12180158071384747	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	777	0.11683929409217221	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	728	0.10947105032059379	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	726	0.10917030567685589	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	704	0.10586211459573905	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.5037232186894753E-4	0.0	0.0	0.0	0.0
6	1.5037232186894753E-4	0.0	0.0	0.0	0.0
7	1.5037232186894753E-4	0.0	0.0	0.0	0.0
8	1.5037232186894753E-4	0.0	0.0	0.0	0.0
9	1.5037232186894753E-4	0.0	0.0	0.0	0.0
10	1.5037232186894753E-4	0.0	0.0	0.0	0.0
11	1.5037232186894753E-4	0.0	0.0	0.0	0.0
12	1.5037232186894753E-4	0.0	0.0	1.5037232186894753E-4	0.0
13	1.5037232186894753E-4	0.0	0.0	1.5037232186894753E-4	0.0
14	1.5037232186894753E-4	0.0	0.0	1.5037232186894753E-4	0.0
15	1.5037232186894753E-4	0.0	0.0	3.0074464373789505E-4	0.0
16	1.5037232186894753E-4	0.0	0.0	0.0010526062530826325	0.0
17	1.5037232186894753E-4	0.0	0.0	0.0013533508968205277	0.0
18	1.5037232186894753E-4	0.0	0.0	0.0016540955405584226	0.0
19	1.5037232186894753E-4	0.0	0.0	0.0024059571499031604	0.0
20	1.5037232186894753E-4	0.0	0.0	0.0027067017936410554	0.0
21	1.5037232186894753E-4	0.0	0.0	0.004360797334199478	0.0
22	1.5037232186894753E-4	0.0	0.0	0.006315637518495796	0.0
23	1.5037232186894753E-4	0.0	0.0	0.008270477702792113	0.0
24	1.5037232186894753E-4	0.0	0.0	0.012029785749515801	0.0
25	1.5037232186894753E-4	0.0	0.0	0.01443574289941896	0.0
26	1.5037232186894753E-4	0.0	0.0	0.019698774164832126	0.0
27	1.5037232186894753E-4	0.0	0.0	0.04180350547956741	0.0
28	1.5037232186894753E-4	0.0	0.0	0.14150035487867962	0.0
29	1.5037232186894753E-4	0.0	0.0	0.2984890589098608	0.0
30	1.5037232186894753E-4	0.0	0.0	0.49953685324864366	0.0
31	1.5037232186894753E-4	0.0	0.0	1.0613278477510315	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAT	20	0.0018413779	37.0	5
TATACAG	25	0.0054946984	29.6	5
GACCTAG	35	8.865448E-4	26.428572	1
GGTATCA	1050	0.0	25.019047	1
ATACACA	115	5.456968E-12	22.52174	37
CTTATAC	725	0.0	20.924137	37
ATACGGC	45	0.0038241376	20.555557	29
TTCGCTA	55	5.1399623E-4	20.181818	26
TATAACT	55	5.1399623E-4	20.181818	4
CTCTAAT	55	5.1399623E-4	20.181818	1
TGCGGGT	395	0.0	20.13924	21
ATAAGCC	75	9.256144E-6	19.733334	3
CAGTCGG	280	0.0	19.160715	10
CTCTATG	495	0.0	19.060606	1
TTCTGCG	420	0.0	18.940477	18
TAATACT	100	2.8704017E-7	18.5	4
CGTTATT	50	0.0070318407	18.5	10
ACAATTA	50	0.0070318407	18.5	35
TACTAGG	50	0.0070318407	18.5	2
CTAGGAC	70	1.21821686E-4	18.5	3
>>END_MODULE