FastQCFastQC Report
Fri 10 Feb 2017
ERR1631779.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631779.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences943102
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17630.18693630169377223No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17170.18205878049246No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13910.14749199980489913No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC13330.14134208176846194No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT10870.1152579466484007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10350.029.4927541
GTGCTAG400.00193093223.1251
CTTATAC12350.020.22267337
GGCCTAT951.6753074E-719.4736846
CCGTGAC609.235141E-418.59
GCATTAT609.235141E-418.51
ACAAGTC801.6161697E-518.516
TGCGTGT500.007033968318.516
TATCCGG701.2189031E-418.510
CCAGGAC5750.018.339133
CTCTATG2600.017.7884621
CGGAATG852.7222766E-517.41176414
GCCTATC852.7222766E-517.4117647
GTGTTTA752.0667705E-417.2666661
CTATCCG752.0667705E-417.2666669
AGGCCAT4950.017.19191725
AGCGTAC1301.3920726E-817.0769231
ACTGAGC1301.3920726E-817.0769238
CTAAACA650.001579649417.0769234
GTATCAA17800.017.0449452
Produced by FastQC (version 0.11.5)