##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631779.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	943102
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16458028930063	33.0	31.0	34.0	30.0	34.0
2	32.3511242686369	34.0	31.0	34.0	31.0	34.0
3	32.43521167381683	34.0	31.0	34.0	30.0	34.0
4	36.02489338374853	37.0	35.0	37.0	35.0	37.0
5	35.84173822131646	37.0	35.0	37.0	35.0	37.0
6	35.867663306832135	37.0	35.0	37.0	35.0	37.0
7	35.85340716062525	37.0	35.0	37.0	35.0	37.0
8	35.825510920345835	37.0	35.0	37.0	35.0	37.0
9	37.599210901896086	39.0	37.0	39.0	35.0	39.0
10	37.476581536249526	39.0	37.0	39.0	35.0	39.0
11	37.563730116148626	39.0	37.0	39.0	35.0	39.0
12	37.491404959378734	39.0	37.0	39.0	35.0	39.0
13	37.55340991748506	39.0	37.0	39.0	35.0	39.0
14	38.81665397804267	40.0	38.0	41.0	36.0	41.0
15	38.79298421591726	40.0	38.0	41.0	36.0	41.0
16	38.74891263087132	40.0	38.0	41.0	35.0	41.0
17	38.69952984936942	40.0	38.0	41.0	35.0	41.0
18	38.69944502291375	40.0	38.0	41.0	35.0	41.0
19	38.741234776302036	40.0	38.0	41.0	35.0	41.0
20	38.70653969560026	40.0	38.0	41.0	35.0	41.0
21	38.68611772639651	40.0	38.0	41.0	35.0	41.0
22	38.64065180648541	40.0	38.0	41.0	35.0	41.0
23	38.58143445777869	40.0	38.0	41.0	35.0	41.0
24	38.59191582670803	40.0	38.0	41.0	35.0	41.0
25	38.51606613070484	40.0	38.0	41.0	34.0	41.0
26	38.43124497668333	40.0	38.0	41.0	34.0	41.0
27	38.336490644702266	40.0	38.0	41.0	34.0	41.0
28	38.29840568676559	40.0	38.0	41.0	34.0	41.0
29	38.258573303842	40.0	38.0	41.0	34.0	41.0
30	38.2001098502601	40.0	38.0	41.0	34.0	41.0
31	38.11710928404351	40.0	38.0	41.0	34.0	41.0
32	38.06017482732515	40.0	38.0	41.0	33.0	41.0
33	37.99328704636402	40.0	37.0	41.0	33.0	41.0
34	37.93780630302979	40.0	37.0	41.0	33.0	41.0
35	37.84450038277938	40.0	37.0	41.0	33.0	41.0
36	37.80861879202886	40.0	37.0	41.0	33.0	41.0
37	37.76542410046845	40.0	37.0	41.0	33.0	41.0
38	37.72495976045009	40.0	37.0	41.0	33.0	41.0
39	37.65783234475168	40.0	37.0	41.0	33.0	41.0
40	37.33043085477499	39.0	36.0	41.0	32.0	41.0
41	37.403340253758344	39.0	36.0	41.0	32.0	41.0
42	37.37512591427014	39.0	36.0	41.0	32.0	41.0
43	36.843538662838164	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	4.0
17	4.0
18	16.0
19	19.0
20	82.0
21	164.0
22	305.0
23	512.0
24	884.0
25	1413.0
26	2274.0
27	3510.0
28	5125.0
29	7346.0
30	9915.0
31	13568.0
32	18414.0
33	24755.0
34	35103.0
35	51952.0
36	79725.0
37	142373.0
38	290043.0
39	255595.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.58372901340471	17.494078053063188	12.948546392648938	26.973646540883173
2	20.025829655753036	19.50891844148353	34.516202913364616	25.949048989398815
3	20.760532794968096	21.121575396934798	29.13004107721116	28.98785073088595
4	15.734247197015804	15.743260007931273	33.808326140756776	34.71416665429614
5	16.51306009318186	33.765382747571316	33.48693990681814	16.234617252428688
6	34.809914516139294	33.99155128501477	15.120633823276803	16.077900375569133
7	30.396500060438854	28.489919436073723	20.471486647255546	20.642093856231885
8	27.05211101238254	32.63188923361418	19.61092225443271	20.705077499570564
9	27.888923997616377	13.776558633106491	18.37690939050071	39.95760797877642
10	18.77697216207791	24.657990334025374	30.98858872105032	25.576448782846395
11	37.29342107216399	20.643896418414975	20.060926601788566	22.00175590763247
12	23.42196284177109	24.155923749498996	27.320798810733095	25.101314597996822
13	32.18549001062451	18.619407020661605	22.672309039743315	26.522793928970568
14	23.715144279197798	20.009712629174786	24.22749607147477	32.047647020152645
15	27.881183583536036	24.98860144501867	21.642727933988052	25.48748703745724
16	25.54368456434193	24.598399748913693	22.873347739693056	26.984567947051325
17	25.49141026103221	24.612608180239253	23.55471624490246	26.341265313826078
18	25.374985950618278	22.971322295997677	25.02221392807989	26.631477825304156
19	27.00916761919708	23.033775773988392	24.63169413276613	25.325362474048408
20	27.169171521214036	22.70867838261397	24.744937451092248	25.37721264507975
21	26.88129173726702	23.417509452848154	23.491096403146212	26.21010240673861
22	26.834107021297804	24.08753241961103	23.115103138366795	25.96325742072438
23	26.562662363137818	23.712493452457952	23.859137187706104	25.86570699669813
24	26.95551488598264	23.478690534003746	23.46130111059037	26.104493469423247
25	26.47274632012232	23.219651744986226	24.484732298309197	25.822869636582258
26	26.56563128908644	23.66626303411508	24.113404488591904	25.65470118820658
27	26.83347082288024	23.104393798337824	23.958490173915443	26.103645204866492
28	25.44274108208868	23.57168153603746	24.36226410292842	26.62331327894544
29	26.276691174443485	23.87260338754451	24.267046406433238	25.58365903157877
30	25.744935330430856	23.29228439765794	25.07851748803417	25.884262783877038
31	26.05465792671418	23.51612020757034	24.31433715547205	26.11488471024343
32	24.52619122852035	23.424295569302153	24.763917370549528	27.28559583162797
33	25.27170974083397	23.311582416324004	25.46352356372906	25.953184279112968
34	25.625754160207485	22.955629401697802	25.42821455155434	25.990401886540376
35	25.591187379519926	23.616851623684397	25.38940644808303	25.40255454871265
36	25.486638772900495	22.975669651851018	25.306276521521532	26.23141505372696
37	24.98828334580989	22.4759357948557	25.620876639006173	26.914904220328236
38	25.13482104798845	21.906114078858916	26.61239187277728	26.346673000375358
39	24.109587298086527	21.667751738412175	27.513354865115332	26.709306098385966
40	24.031440925795938	21.966658961596945	28.20903783471989	25.79286227788723
41	22.938452044423617	21.345623272986376	28.927517914287108	26.7884067683029
42	21.96538656476182	22.405847935854233	28.85562749310255	26.7731380062814
43	21.74600414377236	21.815561837425857	29.141174549518507	27.29725946928328
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	40.0
1	42.0
2	44.0
3	90.0
4	136.0
5	136.0
6	172.5
7	209.0
8	219.5
9	230.0
10	353.5
11	477.0
12	477.0
13	939.0
14	1401.0
15	2458.0
16	3515.0
17	3443.0
18	3371.0
19	3371.0
20	3927.5
21	4484.0
22	4130.5
23	3777.0
24	4267.5
25	4758.0
26	4758.0
27	5772.5
28	6787.0
29	8902.5
30	11018.0
31	13257.0
32	15496.0
33	15496.0
34	18890.5
35	22285.0
36	25404.5
37	28524.0
38	33135.5
39	37747.0
40	37747.0
41	42091.5
42	46436.0
43	51087.0
44	55738.0
45	60554.5
46	65371.0
47	65371.0
48	69029.5
49	72688.0
50	79738.5
51	86789.0
52	88545.0
53	90301.0
54	90301.0
55	85635.5
56	80970.0
57	74734.0
58	68498.0
59	62478.5
60	56459.0
61	56459.0
62	53518.0
63	50577.0
64	41660.0
65	32743.0
66	28575.0
67	24407.0
68	24407.0
69	21283.5
70	18160.0
71	16533.5
72	14907.0
73	14925.5
74	14944.0
75	14944.0
76	12643.5
77	10343.0
78	7452.5
79	4562.0
80	3265.5
81	1969.0
82	1969.0
83	1627.5
84	1286.0
85	1052.5
86	819.0
87	643.5
88	468.0
89	468.0
90	371.5
91	275.0
92	174.0
93	73.0
94	45.0
95	17.0
96	17.0
97	10.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	943102.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.17133874126895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.6366632587571	45.721220450271346
2	5.846092838485787	6.099969795790969
3	1.9361650516077227	3.0303696829929896
4	1.0000207266158878	2.0868968030626953
5	0.6591703330919324	1.7194899367967142
6	0.4222524702174343	1.3217685994830806
7	0.3114557225416461	1.1374343402538825
8	0.24983633825421536	1.0427436986351135
9	0.1877661693891392	0.881641118461614
>10	1.431539491653157	15.023138139864503
>50	0.1795625044920312	6.536777717352836
>100	0.1333398267971178	12.984467514601587
>500	0.005112723414056431	1.6361685925177012
>1k	0.0010225446828112863	0.7779136099150024
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1763	0.18693630169377223	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1717	0.18205878049246	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1391	0.14749199980489913	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1333	0.14134208176846194	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	1087	0.1152579466484007	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	1.060330695937449E-4	0.0	0.0	0.0	0.0
11	2.120661391874898E-4	0.0	0.0	0.0	0.0
12	2.120661391874898E-4	0.0	0.0	0.0	0.0
13	2.120661391874898E-4	0.0	0.0	0.0	0.0
14	2.120661391874898E-4	0.0	0.0	1.060330695937449E-4	0.0
15	3.1809920878123467E-4	0.0	0.0	4.241322783749796E-4	0.0
16	3.1809920878123467E-4	0.0	0.0	0.001060330695937449	1.060330695937449E-4
17	3.1809920878123467E-4	0.0	0.0	0.0014844629743124285	1.060330695937449E-4
18	3.1809920878123467E-4	0.0	0.0	0.002120661391874898	1.060330695937449E-4
19	3.1809920878123467E-4	0.0	0.0	0.002438760600656133	1.060330695937449E-4
20	4.241322783749796E-4	0.0	0.0	0.003287025157406092	1.060330695937449E-4
21	4.241322783749796E-4	0.0	0.0	0.005089587340499755	1.060330695937449E-4
22	4.241322783749796E-4	0.0	0.0	0.006680083384405929	1.060330695937449E-4
23	4.241322783749796E-4	0.0	0.0	0.008482645567499591	1.060330695937449E-4
24	4.241322783749796E-4	0.0	0.0	0.010391240820187	1.060330695937449E-4
25	4.241322783749796E-4	0.0	0.0	0.012087769933686918	1.060330695937449E-4
26	4.241322783749796E-4	0.0	0.0	0.01473859667353054	2.120661391874898E-4
27	4.241322783749796E-4	1.060330695937449E-4	0.0	0.03658140900984199	2.120661391874898E-4
28	7.422314871562142E-4	1.060330695937449E-4	0.0	0.13847918888943084	2.120661391874898E-4
29	7.422314871562142E-4	1.060330695937449E-4	0.0	0.279715237588299	2.120661391874898E-4
30	8.482645567499592E-4	1.060330695937449E-4	0.0	0.461774018080759	2.120661391874898E-4
31	8.482645567499592E-4	1.060330695937449E-4	0.0	1.0737968957758546	2.120661391874898E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1035	0.0	29.492754	1
GTGCTAG	40	0.001930932	23.125	1
CTTATAC	1235	0.0	20.222673	37
GGCCTAT	95	1.6753074E-7	19.473684	6
CCGTGAC	60	9.235141E-4	18.5	9
GCATTAT	60	9.235141E-4	18.5	1
ACAAGTC	80	1.6161697E-5	18.5	16
TGCGTGT	50	0.0070339683	18.5	16
TATCCGG	70	1.2189031E-4	18.5	10
CCAGGAC	575	0.0	18.33913	3
CTCTATG	260	0.0	17.788462	1
CGGAATG	85	2.7222766E-5	17.411764	14
GCCTATC	85	2.7222766E-5	17.411764	7
GTGTTTA	75	2.0667705E-4	17.266666	1
CTATCCG	75	2.0667705E-4	17.266666	9
AGGCCAT	495	0.0	17.191917	25
AGCGTAC	130	1.3920726E-8	17.076923	1
ACTGAGC	130	1.3920726E-8	17.076923	8
CTAAACA	65	0.0015796494	17.076923	4
GTATCAA	1780	0.0	17.044945	2
>>END_MODULE