FastQCFastQC Report
Fri 10 Feb 2017
ERR1631778.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631778.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1390182
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38370.2760070264181237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT31760.22845929525774325No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29840.21464815398271594No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA25130.1807676980424146No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA19750.1420677292613485No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA17280.1243002714752457No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA17260.12415640542029749No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG14570.10480642102976445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14150.030.3321551
TCGTAAC400.001931389823.12532
TATACAC4950.021.67676737
CTTATAC17000.021.32941237
ATACGGC1059.840733E-1021.14285929
ATCTATA609.2381414E-418.52
TAGACTC609.2381414E-418.55
TATACTT609.2381414E-418.55
CGGGTAA5000.017.7623
GGTAATA9350.017.60962525
GTATCAA24150.017.465842
GCGGTAA9100.017.2802223
TTAGCTA1502.5102054E-1017.2666664
TAGTTCG752.067659E-417.26666612
CGCGGTA9350.017.21390322
TCTTATA31850.017.19309237
AACTCCG9500.017.1368435
TTTTACG1201.0416261E-716.9583344
TACACTC1005.879572E-616.6500025
TGCGGGT5400.016.44444521
Produced by FastQC (version 0.11.5)