FastQCFastQC Report
Fri 10 Feb 2017
ERR1631777.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631777.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407112
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA11790.2896008960679125No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA11000.2701959166028022No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG9600.23580734539880918No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC9220.22647330464343965No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9120.22401697812886873No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA8970.22033248835701233No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT8560.21026154964727153No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8340.20485763131521548No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8300.2038751007093871No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7940.19503232525693176No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT7720.1896284069248757No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA7400.18176816207824872No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA7300.1793118355636778No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6760.1660476723849948No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6390.15695926428108237No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5760.14148440723928551No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5530.13583485625577235No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA5470.1343610603470298No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5120.12576391754603156No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA5120.12576391754603156No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5100.12527265224311737No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5090.12502701959166027No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4900.12035999921397551No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT4860.11937746860814714No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT4830.11864057065377587No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC4700.11544734618483365No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG4560.11200848906443436No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4480.11004342785277761No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4360.10709583603529249No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4110.10095501974886519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC551.8495484E-830.27272829
CCTAGAC250.00549222729.5999982
GGTATCA7650.029.5032671
CGAGGGT405.930854E-527.7516
TATACAA358.859517E-426.4285725
TTTTACG358.859517E-426.4285724
TATTCTA451.3211624E-424.6666665
TCCAACG451.3211624E-424.6666662
TATACAC1103.6379788E-1223.54545437
GCGGTAA3650.023.3150723
CTATACC400.001929057223.1254
TATGAAT400.001929057223.1252
TTACGAG400.001929057223.1256
ACCGGTA502.6979513E-422.19999928
CCGGTAT502.6979513E-422.19999929
CGCGGTA3850.022.10389522
TAATACG3750.021.70666927
GGTAATA4050.021.01234625
CGGTAAT4050.021.01234624
CCGCGGT4200.020.70238121
Produced by FastQC (version 0.11.5)