FastQCFastQC Report
Fri 10 Feb 2017
ERR1631776.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631776.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002870
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA22740.22674922971073022No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA22580.2251538085694058No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC18940.18885797760427572No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA17700.17649346375901165No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT17660.17609460847368055No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG17530.17479832879635446No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC16140.16093810763109875No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA15750.15704926859912052No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA15730.156849840956455No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA15040.1499695872844935No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT14520.1447844685751892No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13460.13421480351391507No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT11230.11197862135670625No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA11230.11197862135670625No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA11080.11048291403671463No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG10850.10818949614606081No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10790.10759121321806414No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA10570.10539750914874312No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10290.1026055221514254No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT10130.10101010101010101No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG10120.10091038718876824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10200.029.926471
TACGCTA405.9393875E-527.759
TATACGC451.3230482E-424.6666667
GACCGTT802.725028E-823.1257
ATATACG502.70177E-422.1999996
ATACGGC1250.022.19999929
TACGGCT1350.021.92592430
TAATATA705.1008537E-621.1428574
ATACCAT450.003825473820.5555556
CTTATAC7400.020.537
TGCGGGT4700.020.46808621
TATACAC2050.019.85365737
GGTAACG4850.019.83505225
GCGGTAA6700.019.60447723
GTAACGT4950.019.43434326
CCGCTTA1052.2584572E-819.3809525
TTCTGCG5000.019.2418
GCAGTCG2700.019.1851849
AGTCGGT2850.018.8245611
CGGGTAA5200.018.523
Produced by FastQC (version 0.11.5)