##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631776.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1002870
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.591636004666604	34.0	31.0	34.0	31.0	34.0
2	32.76234806106474	34.0	31.0	34.0	31.0	34.0
3	32.85091587144894	34.0	31.0	34.0	31.0	34.0
4	36.277635187013274	37.0	37.0	37.0	35.0	37.0
5	36.19230508440775	37.0	35.0	37.0	35.0	37.0
6	36.24695025277454	37.0	37.0	37.0	35.0	37.0
7	36.23540139798777	37.0	37.0	37.0	35.0	37.0
8	36.21582458344551	37.0	37.0	37.0	35.0	37.0
9	38.05254120673666	39.0	38.0	39.0	35.0	39.0
10	37.98029156321358	39.0	38.0	39.0	35.0	39.0
11	38.056592579297416	39.0	38.0	39.0	35.0	39.0
12	37.99668850399354	39.0	38.0	39.0	35.0	39.0
13	38.04769910357275	39.0	38.0	39.0	35.0	39.0
14	39.44042298603009	40.0	39.0	41.0	37.0	41.0
15	39.44894851775405	40.0	39.0	41.0	37.0	41.0
16	39.39396731380937	40.0	39.0	41.0	37.0	41.0
17	39.361871428998775	40.0	39.0	41.0	36.0	41.0
18	39.37731610278501	40.0	39.0	41.0	36.0	41.0
19	39.40503355370088	40.0	39.0	41.0	37.0	41.0
20	39.39368911224785	40.0	39.0	41.0	37.0	41.0
21	39.36105576994027	40.0	39.0	41.0	36.0	41.0
22	39.33820734492008	40.0	39.0	41.0	36.0	41.0
23	39.27015465613689	40.0	39.0	41.0	36.0	41.0
24	39.27998045609102	40.0	39.0	41.0	36.0	41.0
25	39.230328955896574	40.0	39.0	41.0	36.0	41.0
26	39.17326273594783	40.0	39.0	41.0	36.0	41.0
27	39.0700130625106	40.0	39.0	41.0	36.0	41.0
28	39.05991105527137	40.0	39.0	41.0	35.0	41.0
29	39.03860221165256	40.0	39.0	41.0	35.0	41.0
30	39.00191749678423	40.0	39.0	41.0	35.0	41.0
31	38.918708307158454	40.0	38.0	41.0	35.0	41.0
32	38.86000378912521	40.0	38.0	41.0	35.0	41.0
33	38.80519110153858	40.0	38.0	41.0	35.0	41.0
34	38.77712066369519	40.0	38.0	41.0	35.0	41.0
35	38.71191281023463	40.0	38.0	41.0	35.0	41.0
36	38.66387767108399	40.0	38.0	41.0	35.0	41.0
37	38.615580284583245	40.0	38.0	41.0	35.0	41.0
38	38.57933032197593	40.0	38.0	41.0	35.0	41.0
39	38.50546631168546	40.0	38.0	41.0	35.0	41.0
40	38.180858934856964	40.0	38.0	41.0	34.0	41.0
41	38.26097101319214	40.0	37.0	41.0	34.0	41.0
42	38.252752600037894	40.0	37.0	41.0	34.0	41.0
43	37.784977115678004	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	3.0
13	1.0
14	0.0
15	1.0
16	1.0
17	1.0
18	10.0
19	15.0
20	47.0
21	94.0
22	180.0
23	318.0
24	516.0
25	831.0
26	1442.0
27	2098.0
28	3169.0
29	4774.0
30	6730.0
31	9284.0
32	12539.0
33	17310.0
34	25360.0
35	37817.0
36	58149.0
37	103859.0
38	240474.0
39	477846.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.76446598262985	16.46883444514244	12.751403472035259	28.01529610019245
2	20.264740195638517	19.61949205779413	32.990517215591254	27.125250530976096
3	20.80628595929682	20.03460069600247	29.37010779064086	29.789005554059848
4	15.528632823795707	16.02670336135292	33.885349048231575	34.559314766619806
5	16.57911793153649	33.04077298154297	33.850748352229104	16.529360734691437
6	34.11060257062231	33.592389841155885	15.846221344740593	16.450786243481208
7	30.319483083550207	27.969128600915372	20.464167838304068	21.24722047723035
8	27.07609161705904	31.179813934009392	20.632285341071128	21.11180910786044
9	28.321616959326732	13.200414809496744	18.08080808080808	40.39716015036844
10	18.927876993030004	24.04399373797202	30.46755810822938	26.560571160768593
11	38.17842791189287	19.92740833806974	19.320749449081138	22.573414300956255
12	22.520565975649884	23.842571818879815	27.11388315534416	26.52297905012614
13	33.99513396551896	17.931037921166254	21.807512439299213	26.266315674015576
14	23.966516098796454	20.39596358451245	23.415896377396873	32.221623939294226
15	28.995682391536292	24.582248945526338	20.446817633392165	25.975251029545205
16	25.520556004267753	24.41223688015396	22.60472444085475	27.462482674723542
17	26.401926471028148	24.267352697757435	22.301793851645776	27.02892697956864
18	26.062799764675383	22.200484609171678	23.983467448423028	27.753248177729915
19	27.503165913827317	22.843937898232074	23.772572716304207	25.8803234716364
20	29.188130066708545	21.372859892109645	23.45657961650064	25.982430424681162
21	26.81304655638318	22.88801140726116	22.431820674663715	27.867121361691943
22	27.79442998594035	23.26672450068304	21.80452102465923	27.134324488717382
23	27.311914804511055	22.829579107960154	22.91742698455433	26.941079102974463
24	27.38251219001466	22.39502627459192	23.238106633960534	26.984354901432887
25	27.45081615762761	22.42454156570642	23.585310159841256	26.539332116824717
26	27.284692931287207	23.262137664901733	22.830476532352147	26.622692871458913
27	26.716523577333056	22.21494311326493	24.1132948437983	26.955238465603713
28	25.89617796922831	23.27898930070697	22.710720232931486	28.114112497133227
29	26.791608084796632	23.510125938556342	22.90266933899708	26.795596637649943
30	26.407909300308113	22.888609690189156	23.76619103173891	26.937289977763818
31	27.488807123555397	22.800462672130983	23.113165215830566	26.597564988483054
32	25.367894143807273	22.944648857778176	23.185258308654163	28.502198689760387
33	25.596139080837993	22.393031998165267	24.850080269626172	27.160748651370564
34	26.09610418100053	22.273375412565937	24.134035318635515	27.496485087798018
35	25.646893415896376	23.935604814183296	24.632404997656725	25.785096772263604
36	25.85509587483921	23.03169902380169	24.856661381834137	26.25654371952496
37	26.59955926490971	22.700349995512877	24.365371384127556	26.33471935544986
38	25.70412914934139	22.35653673955747	25.189306689800272	26.750027421300864
39	25.4820664692333	21.51664722247151	25.876235204961763	27.12505110333343
40	24.933540738081707	22.469213357663506	26.602151824264364	25.995094079990427
41	23.5598831354014	21.73691505379561	27.390289868078614	27.312911942724384
42	22.712814223179475	21.903636563064005	27.996151046496554	27.387398167259963
43	22.177849571729137	22.389741442061283	28.004527007488505	27.427881978721068
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	9.0
2	8.0
3	23.5
4	39.0
5	39.0
6	61.0
7	83.0
8	83.5
9	84.0
10	146.0
11	208.0
12	208.0
13	399.5
14	591.0
15	1194.5
16	1798.0
17	1789.5
18	1781.0
19	1781.0
20	2134.5
21	2488.0
22	2380.0
23	2272.0
24	2637.5
25	3003.0
26	3003.0
27	3488.0
28	3973.0
29	5591.0
30	7209.0
31	8593.0
32	9977.0
33	9977.0
34	12533.0
35	15089.0
36	17028.0
37	18967.0
38	23600.5
39	28234.0
40	28234.0
41	32891.5
42	37549.0
43	42324.5
44	47100.0
45	62401.5
46	77703.0
47	77703.0
48	84368.0
49	91033.0
50	102024.5
51	113016.0
52	115453.5
53	117891.0
54	117891.0
55	105862.5
56	93834.0
57	87708.0
58	81582.0
59	73721.5
60	65861.0
61	65861.0
62	60750.5
63	55640.0
64	46758.5
65	37877.0
66	33131.5
67	28386.0
68	28386.0
69	24454.0
70	20522.0
71	17584.5
72	14647.0
73	11961.0
74	9275.0
75	9275.0
76	7643.0
77	6011.0
78	4997.5
79	3984.0
80	3128.0
81	2272.0
82	2272.0
83	1833.0
84	1394.0
85	1074.5
86	755.0
87	596.0
88	437.0
89	437.0
90	315.0
91	193.0
92	133.5
93	74.0
94	45.5
95	17.0
96	17.0
97	9.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1002870.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.40553200942772
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.94529143385168	38.56986149249919
2	7.369695910319776	6.692499271115464
3	2.3362272107849344	3.1823291820177415
4	1.2346200243992025	2.242343161493537
5	0.7378503815298256	1.6751244558360474
6	0.49640988331543295	1.3523852908005105
7	0.357870431498985	1.1374508132858412
8	0.2778287962928518	1.0091971442572687
9	0.20675953006644618	0.8449223814617631
>10	1.6221663443483465	14.82543686276385
>50	0.22894614725760598	7.252216979272079
>100	0.16933424900849856	14.405056921846409
>500	0.012363387146455287	3.762957735779624
>1k	0.004636270179920733	3.0482183075706435
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2274	0.22674922971073022	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	2258	0.2251538085694058	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1894	0.18885797760427572	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1770	0.17649346375901165	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1766	0.17609460847368055	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1753	0.17479832879635446	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1614	0.16093810763109875	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1575	0.15704926859912052	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1573	0.156849840956455	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1504	0.1499695872844935	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1452	0.1447844685751892	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1346	0.13421480351391507	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1123	0.11197862135670625	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	1123	0.11197862135670625	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1108	0.11048291403671463	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1085	0.10818949614606081	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1079	0.10759121321806414	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1057	0.10539750914874312	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1029	0.1026055221514254	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	1013	0.10101010101010101	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	1012	0.10091038718876824	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	3.988552853310997E-4	0.0	0.0	0.0	0.0
9	3.988552853310997E-4	0.0	0.0	0.0	0.0
10	4.985691066638747E-4	0.0	0.0	9.971382133277493E-5	0.0
11	4.985691066638747E-4	0.0	0.0	1.9942764266554986E-4	0.0
12	5.982829279966496E-4	0.0	0.0	1.9942764266554986E-4	0.0
13	5.982829279966496E-4	0.0	0.0	1.9942764266554986E-4	0.0
14	5.982829279966496E-4	0.0	0.0	1.9942764266554986E-4	0.0
15	6.979967493294246E-4	0.0	0.0	1.9942764266554986E-4	0.0
16	6.979967493294246E-4	0.0	0.0	1.9942764266554986E-4	0.0
17	6.979967493294246E-4	0.0	0.0	2.991414639983248E-4	0.0
18	8.974243919949744E-4	0.0	0.0	3.988552853310997E-4	0.0
19	8.974243919949744E-4	0.0	0.0	5.982829279966496E-4	0.0
20	9.971382133277494E-4	0.0	0.0	5.982829279966496E-4	0.0
21	9.971382133277494E-4	0.0	0.0	0.0012962796773260741	0.0
22	9.971382133277494E-4	0.0	0.0	0.0023931317119865983	0.0
23	9.971382133277494E-4	0.0	0.0	0.003091128461316023	0.0
24	9.971382133277494E-4	0.0	0.0	0.004786263423973197	0.0
25	9.971382133277494E-4	0.0	0.0	0.005384546351969847	0.0
26	9.971382133277494E-4	0.0	0.0	0.0074785365999581205	0.0
27	9.971382133277494E-4	0.0	0.0	0.02054104719455164	0.0
28	9.971382133277494E-4	0.0	0.0	0.07528393510624508	0.0
29	9.971382133277494E-4	0.0	0.0	0.17001206537238125	0.0
30	9.971382133277494E-4	0.0	0.0	0.29136378593436835	0.0
31	9.971382133277494E-4	0.0	0.0	0.6614017769002961	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1020	0.0	29.92647	1
TACGCTA	40	5.9393875E-5	27.75	9
TATACGC	45	1.3230482E-4	24.666666	7
GACCGTT	80	2.725028E-8	23.125	7
ATATACG	50	2.70177E-4	22.199999	6
ATACGGC	125	0.0	22.199999	29
TACGGCT	135	0.0	21.925924	30
TAATATA	70	5.1008537E-6	21.142857	4
ATACCAT	45	0.0038254738	20.555555	6
CTTATAC	740	0.0	20.5	37
TGCGGGT	470	0.0	20.468086	21
TATACAC	205	0.0	19.853657	37
GGTAACG	485	0.0	19.835052	25
GCGGTAA	670	0.0	19.604477	23
GTAACGT	495	0.0	19.434343	26
CCGCTTA	105	2.2584572E-8	19.38095	25
TTCTGCG	500	0.0	19.24	18
GCAGTCG	270	0.0	19.185184	9
AGTCGGT	285	0.0	18.82456	11
CGGGTAA	520	0.0	18.5	23
>>END_MODULE