FastQCFastQC Report
Fri 10 Feb 2017
ERR1631775.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631775.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194804
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9280.47637625510769793No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7730.39680910042914935No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA7690.39475575450196093No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7230.3711422763392949No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7160.36754892096671526No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7130.366008911521324No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7110.3649822385577298No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA6380.3275086753865424No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6330.32494199297755694No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6270.3218619740867744No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT6130.31467526334161516No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5980.30697521611465883No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5750.2951684770333258No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5670.2910617851789491No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5280.2710416623888626No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4990.2561549044167471No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA4830.2479415207079937No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4820.24742818422619658No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4790.24588817478080532No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4590.23562144514486355No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA4450.2284347343997043No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4140.2125213034639946No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4110.21098129401860333No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT4110.21098129401860333No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC3900.20020122790086445No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3860.1981478819736761No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3780.19404119011929938No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3780.19404119011929938No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3700.18993449826492267No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA3680.18890782530132852No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC3680.18890782530132852No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3670.1883944888195314No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA3510.180181105110778No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT3510.180181105110778No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3500.17966776862898093No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3430.1760744132564013No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA3420.17556107677460422No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA3190.1637543376932712No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG3170.162727664729677No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3160.16221432824787993No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3090.1586209728753003No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3070.15759429991170612No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC3020.15502761750272068No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT2970.15246093509373523No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC2960.15194759861193816No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT2910.1493809162029527No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2820.1447608878667789No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT2780.14270754193959057No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA2690.13808751360341676No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG2640.1355208311944313No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC2630.13500749471263424No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT2590.1329541487854459No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG2550.13090080285825753No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT2550.13090080285825753No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG2540.13038746637646045No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT2520.12936079341286627No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2500.1283341204492721No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA2500.1283341204492721No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG2470.12679411100388083No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC2470.12679411100388083No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC2420.12422742859489538No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC2420.12422742859489538No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG2410.12371409211309829No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT2410.12371409211309829No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC2400.1232007556313012No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA2360.12114740970411285No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG2340.12012073674051867No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT2300.11806739081333033No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC2270.11652738136793907No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG2240.11498737192254778No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2200.11293402599535944No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA2180.11190735303176526No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT2180.11190735303176526No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC2170.11139401654996818No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2170.11139401654996818No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT2160.11088068006817108No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT2160.11088068006817108No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG2160.11088068006817108No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC2130.10934067062277981No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC2050.10523397876840311No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA2040.10472064228660603No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT2040.10472064228660603No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC2030.10420730580480894No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG1990.10215395987762058No Hit
CACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCA1980.1016406233958235No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT1970.1011272869140264No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC1970.1011272869140264No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG1970.1011272869140264No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC1960.10061395043222932No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGCT200.00183818637.02
ACTCGAC200.00183818637.016
CAACTCG200.00183818637.014
GCAACTC200.00183818637.013
AACTCGA200.00183818637.015
TCTGCAA200.00183818637.010
TCCATGA200.00183818637.023
ATCGCTA200.00183818637.037
GGAATCG200.00183818637.034
CGACTCC200.00183818637.019
ATACACA507.2668627E-933.337
CTCGACT250.00548528329.59999817
GCGACGC250.00548528329.59999830
ATTGGAG250.00548528329.5999983
ACTCCAT250.00548528329.59999821
CGGAATC250.00548528329.59999833
AGTCGGA250.00548528329.59999830
GAATCGC250.00548528329.59999835
GAGGGCT250.00548528329.59999834
AGCGACG250.00548528329.59999829
Produced by FastQC (version 0.11.5)