FastQCFastQC Report
Fri 10 Feb 2017
ERR1631774.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631774.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210829
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4060.19257312798523923No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA3700.17549767821314904No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA3120.14798723135811487No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2970.14087246061974396No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2930.13897518842284504No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2910.13802655232439562No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2650.12569428304455268No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2610.12379701084765378No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2510.11905383035540652No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2400.11383633181393453No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2360.1119390596170356No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2250.1067215610755636No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2220.10529860692788943No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAAGA200.001838528937.012
CGTCAGA251.2302084E-437.01
CTAGAAC250.005486292829.63
GGTATCA2700.029.4629611
TCTAGCA358.845291E-426.428573
GCGGTAA1103.6379788E-1223.54545423
GTATTAG400.001925988523.1251
TAGAGGT502.691972E-422.24
TAACTCC1250.022.24
GGTAATA1350.021.92592425
ACTATCG603.707737E-521.58333411
CTTATTG603.707737E-521.58333428
AACTCCG1201.0913936E-1121.5833345
GCTAACT1301.8189894E-1221.3461532
TGGTTCA705.069387E-621.1428575
AGTCGGT705.069387E-621.14285711
CTCTATG806.904793E-720.81251
AGCCGCG1251.8189894E-1120.72000119
CGGTAAT1251.8189894E-1120.72000124
AGGACTT450.003815607220.5555555
Produced by FastQC (version 0.11.5)