##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631774.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	210829
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86611898742583	31.0	31.0	34.0	30.0	34.0
2	32.06672231998444	33.0	31.0	34.0	30.0	34.0
3	32.14092937878565	34.0	31.0	34.0	30.0	34.0
4	35.839163492688385	37.0	35.0	37.0	35.0	37.0
5	35.50507283153646	37.0	35.0	37.0	33.0	37.0
6	35.50715034459206	37.0	35.0	37.0	33.0	37.0
7	35.51813555061211	37.0	35.0	37.0	33.0	37.0
8	35.47656157359756	37.0	35.0	37.0	33.0	37.0
9	37.2149277376452	39.0	37.0	39.0	34.0	39.0
10	37.022677145933436	39.0	37.0	39.0	33.0	39.0
11	37.124532203823954	39.0	37.0	39.0	33.0	39.0
12	37.03894151184135	39.0	37.0	39.0	33.0	39.0
13	37.13693562081118	39.0	37.0	39.0	34.0	39.0
14	38.28126586000977	40.0	38.0	41.0	34.0	41.0
15	38.2569001418211	40.0	38.0	41.0	34.0	41.0
16	38.19841672635169	40.0	38.0	41.0	34.0	41.0
17	38.1554577406334	40.0	38.0	41.0	33.0	41.0
18	38.160627807369956	40.0	38.0	41.0	33.0	41.0
19	38.17932542487039	40.0	38.0	41.0	34.0	41.0
20	38.14550654796067	40.0	38.0	41.0	34.0	41.0
21	38.12081355031803	40.0	38.0	41.0	34.0	41.0
22	38.088061889019066	40.0	38.0	41.0	34.0	41.0
23	38.001048242888785	40.0	37.0	41.0	33.0	41.0
24	38.016672279430246	40.0	37.0	41.0	33.0	41.0
25	37.94089522788611	40.0	37.0	41.0	33.0	41.0
26	37.82155680670117	39.0	37.0	41.0	33.0	41.0
27	37.72018555322086	39.0	37.0	40.0	33.0	41.0
28	37.689573066323895	39.0	37.0	40.0	33.0	41.0
29	37.643118356582825	39.0	37.0	40.0	33.0	41.0
30	37.60149220458286	39.0	37.0	40.0	33.0	41.0
31	37.490686765103476	39.0	37.0	40.0	33.0	41.0
32	37.451565012403414	39.0	37.0	40.0	32.0	41.0
33	37.374583193014246	39.0	36.0	40.0	32.0	41.0
34	37.33953583235703	39.0	36.0	40.0	32.0	41.0
35	37.24518922918574	39.0	36.0	40.0	32.0	41.0
36	37.19549967034896	39.0	36.0	40.0	32.0	41.0
37	37.12586029436178	39.0	36.0	40.0	31.0	41.0
38	37.09276712406737	39.0	36.0	40.0	31.0	41.0
39	37.01999724895531	39.0	36.0	40.0	31.0	41.0
40	36.724876558727686	39.0	35.0	40.0	31.0	41.0
41	36.78557029630649	39.0	35.0	40.0	31.0	41.0
42	36.7524012351242	39.0	35.0	40.0	31.0	41.0
43	36.20521370399708	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	3.0
18	11.0
19	23.0
20	32.0
21	57.0
22	89.0
23	181.0
24	269.0
25	437.0
26	621.0
27	1058.0
28	1450.0
29	2130.0
30	2928.0
31	3856.0
32	5310.0
33	7202.0
34	10244.0
35	15131.0
36	24444.0
37	41270.0
38	61450.0
39	32631.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.44529927097316	17.28841857619208	12.893387532075758	27.372894620759002
2	20.939244601074805	19.80704741757538	32.79624719559454	26.45746078575528
3	20.930706876188758	20.451645646471786	29.199493428323425	29.418154049016028
4	15.957482130067497	16.154324120495758	33.69840012521996	34.18979362421678
5	16.773783492783252	33.09601620270456	33.777611239440496	16.352589065071694
6	34.924512282465884	33.71120671254903	15.237467331344359	16.126813673640722
7	30.638574389671252	27.954408549108518	20.057013029516813	21.350004031703417
8	27.63092363953725	31.40744394746453	20.13432687154044	20.82730554145777
9	28.4163943290534	12.937499110653658	17.877047275279967	40.76905928501297
10	18.85461677473213	24.19259210070721	30.705453234611934	26.247337889948724
11	38.719530994312926	19.765307429243606	19.083238074458446	22.431923501985022
12	22.993041754217874	23.961599210734764	27.119608782472998	25.92575025257436
13	34.39612197562954	17.930170896793136	21.43348400836697	26.240223119210356
14	24.212513458774644	20.1642089086416	23.303245758410846	32.320031874172905
15	28.93861850124983	24.564457451299393	20.42271224546908	26.0742118019817
16	25.65491464646704	24.400817724316866	22.448998951757112	27.495268677458984
17	26.537620536074257	24.02326055713398	22.35555829605984	27.083560610731922
18	26.42615579450645	22.070967466525005	23.863889692594473	27.638987046374076
19	27.786499959682963	22.630662764610182	23.566017957681343	26.016819318025508
20	29.08138823406647	21.531193526507263	23.304668712558517	26.082749526867744
21	27.04182062240014	22.849797703352007	22.364096020945883	27.744285653301965
22	27.89796470125078	23.415184818027882	21.795862997974663	26.89098748274668
23	27.56831365703959	22.874936559960915	22.593665956770653	26.96308382622884
24	27.487205270622162	22.317138534072637	22.947507221492298	27.248148973812903
25	27.50807526478805	22.43808963662494	23.28949053498333	26.764344563603682
26	27.186487627413687	23.144349211920563	22.751613867162487	26.917549293503267
27	26.950277238899773	22.35555829605984	23.966816709276237	26.727347755764153
28	26.436590791589392	23.001579479103917	22.660070483662114	27.901759245644577
29	26.764344563603682	23.334550749659677	22.993041754217874	26.908062932518774
30	26.602602108818047	22.82797907308767	23.756219495420456	26.813199322673825
31	27.42791551446907	22.88632019314231	22.9897215278733	26.696042764515315
32	25.504555824862802	22.913356321948118	23.145297848019013	28.436790005170064
33	25.55530785612985	22.651532758776067	24.782169435893543	27.010989949200535
34	26.18757381574641	22.135474721219566	24.256625037352546	27.420326425681473
35	25.803850513923603	23.57360704646894	24.69631786898387	25.926224570623585
36	25.91484093744219	22.632085718757857	24.96952506533731	26.483548278462642
37	26.39911966570064	22.453742132249356	24.600031304991248	26.547106897058754
38	25.55673081027752	21.946696137628123	25.57807512249264	26.918497929601713
39	25.172058872356267	21.2978290462887	26.245440617751825	27.284671463603203
40	24.60714607572962	22.097529277281588	27.103007650750133	26.192316996238656
41	23.54325069131856	20.98477913380038	27.984764904258903	27.487205270622162
42	22.555720512832675	21.62700577245066	28.484221810092542	27.333051904624128
43	22.113181772906003	21.81673299214055	28.617030863875463	27.453054371077982
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.5
2	2.0
3	4.0
4	6.0
5	6.0
6	11.5
7	17.0
8	14.0
9	11.0
10	30.0
11	49.0
12	49.0
13	92.5
14	136.0
15	258.5
16	381.0
17	376.0
18	371.0
19	371.0
20	465.0
21	559.0
22	512.5
23	466.0
24	564.0
25	662.0
26	662.0
27	844.5
28	1027.0
29	1294.0
30	1561.0
31	1929.5
32	2298.0
33	2298.0
34	2861.0
35	3424.0
36	3958.5
37	4493.0
38	5336.0
39	6179.0
40	6179.0
41	7089.5
42	8000.0
43	9134.5
44	10269.0
45	12765.5
46	15262.0
47	15262.0
48	16542.5
49	17823.0
50	19902.5
51	21982.0
52	22496.5
53	23011.0
54	23011.0
55	21517.0
56	20023.0
57	18910.5
58	17798.0
59	16325.5
60	14853.0
61	14853.0
62	13639.0
63	12425.0
64	10560.0
65	8695.0
66	7548.5
67	6402.0
68	6402.0
69	5395.0
70	4388.0
71	3750.5
72	3113.0
73	2541.5
74	1970.0
75	1970.0
76	1587.5
77	1205.0
78	1005.5
79	806.0
80	649.0
81	492.0
82	492.0
83	401.0
84	310.0
85	251.0
86	192.0
87	147.0
88	102.0
89	102.0
90	74.5
91	47.0
92	31.5
93	16.0
94	8.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	210829.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.219868455863335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.17300991661297	49.5876143333515
2	7.401480118785014	8.618263977887025
3	2.3715583308809642	4.142154421778897
4	1.217200621751832	2.8346104033114687
5	0.7471961603357333	2.1750831082736277
6	0.497544202833287	1.7380174823958812
7	0.39106239593351627	1.5937320874498844
8	0.28381895031271503	1.3219121561989984
9	0.24014192805932305	1.2582928316116067
>10	1.5082965097344612	16.582750101982807
>50	0.11411440851423088	4.595725144136487
>100	0.0545764562459365	5.551843951621827
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	406	0.19257312798523923	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	370	0.17549767821314904	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	312	0.14798723135811487	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	297	0.14087246061974396	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	293	0.13897518842284504	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	291	0.13802655232439562	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	265	0.12569428304455268	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	261	0.12379701084765378	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	251	0.11905383035540652	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	240	0.11383633181393453	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	236	0.1119390596170356	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	225	0.1067215610755636	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	222	0.10529860692788943	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	4.7431804922472715E-4	0.0
16	0.0	0.0	0.0	9.486360984494543E-4	0.0
17	0.0	0.0	0.0	0.0023715902461236356	0.0
18	0.0	0.0	0.0	0.0023715902461236356	0.0
19	0.0	0.0	0.0	0.002845908295348363	0.0
20	0.0	0.0	0.0	0.002845908295348363	0.0
21	4.7431804922472715E-4	0.0	0.0	0.007114770738370907	0.0
22	4.7431804922472715E-4	0.0	0.0	0.009486360984494542	0.0
23	4.7431804922472715E-4	0.0	0.0	0.010909315132168725	0.0
24	4.7431804922472715E-4	0.0	0.0	0.01328090537829236	0.0
25	4.7431804922472715E-4	0.0	0.0	0.014229541476741814	0.0
26	4.7431804922472715E-4	0.0	0.0	0.015652495624415997	0.0
27	4.7431804922472715E-4	0.0	0.0	0.02845908295348363	0.0
28	4.7431804922472715E-4	0.0	0.0	0.09723520009106906	0.0
29	4.7431804922472715E-4	0.0	0.0	0.2148660762988014	0.0
30	4.7431804922472715E-4	0.0	0.0	0.35336694667242174	0.0
31	4.7431804922472715E-4	0.0	0.0	0.8210445432080027	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAAGA	20	0.0018385289	37.0	12
CGTCAGA	25	1.2302084E-4	37.0	1
CTAGAAC	25	0.0054862928	29.6	3
GGTATCA	270	0.0	29.462961	1
TCTAGCA	35	8.845291E-4	26.42857	3
GCGGTAA	110	3.6379788E-12	23.545454	23
GTATTAG	40	0.0019259885	23.125	1
TAGAGGT	50	2.691972E-4	22.2	4
TAACTCC	125	0.0	22.2	4
GGTAATA	135	0.0	21.925924	25
ACTATCG	60	3.707737E-5	21.583334	11
CTTATTG	60	3.707737E-5	21.583334	28
AACTCCG	120	1.0913936E-11	21.583334	5
GCTAACT	130	1.8189894E-12	21.346153	2
TGGTTCA	70	5.069387E-6	21.142857	5
AGTCGGT	70	5.069387E-6	21.142857	11
CTCTATG	80	6.904793E-7	20.8125	1
AGCCGCG	125	1.8189894E-11	20.720001	19
CGGTAAT	125	1.8189894E-11	20.720001	24
AGGACTT	45	0.0038156072	20.555555	5
>>END_MODULE