FastQCFastQC Report
Fri 10 Feb 2017
ERR1631773.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631773.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23006
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1080.46944275406415714No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA910.39554898722072507No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA890.3868556028862036No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC740.3216552203772929No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA710.30861514387551076No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT690.2999217595409893No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG680.29557506737372863No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC640.27818829870468575No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA620.26949491437016426No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT610.26514822220290357No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA610.26514822220290357No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT590.25645483786838214No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA520.22602799269755713No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA510.22168130053029644No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA480.20864122402851432No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA470.20429453186125357No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT440.19125445535947144No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA420.18256107102495No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA410.1782143788576893No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC410.1782143788576893No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA400.17386768669042857No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG400.17386768669042857No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA390.16952099452316788No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT370.16082761018864644No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT370.16082761018864644No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT360.15648091802138572No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG350.152134225854125No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC350.152134225854125No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC350.152134225854125No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG350.152134225854125No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC340.14778753368686431No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA330.1434408415196036No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC330.1434408415196036No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT320.13909414935234288No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA310.13474745718508213No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC310.13474745718508213No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT310.13474745718508213RNA PCR Primer, Index 17 (95% over 21bp)
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT300.13040076501782144No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC300.13040076501782144No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT300.13040076501782144No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT290.12605407285056072No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA290.12605407285056072No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT290.12605407285056072No Hit
CATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACT290.12605407285056072No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA280.1217073806833No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC270.11736068851603929No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG270.11736068851603929No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG270.11736068851603929No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC270.11736068851603929No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA270.11736068851603929No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA270.11736068851603929No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC260.11301399634877857No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG260.11301399634877857No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA250.10866730418151788No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA240.10432061201425716No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG240.10432061201425716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA506.692062E-933.3000031
GTATCAA701.7585444E-723.7857152
ATCAACG953.3063225E-617.5263164
TATCAAC1107.0246097E-716.8181823
TCAACGC1005.391399E-616.6500025
CAGAGTA1005.391399E-616.65000211
AGAGTAC1005.391399E-616.65000212
CAACGCA1005.391399E-616.6500026
AACGCAG1058.57411E-615.8571427
GCAGAGT1058.57411E-615.85714210
AGTACGG956.591716E-515.57894814
GAGTACG956.591716E-515.57894813
ACGCAGA1101.3329649E-515.1363648
CGCAGAG1101.3329649E-515.1363649
CTGTCTC1951.2403507E-712.33333437
GTACGGG1150.00495921811.2608715
Produced by FastQC (version 0.11.5)