##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631773.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23006
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5468138746414	34.0	31.0	34.0	31.0	34.0
2	32.69951317047727	34.0	31.0	34.0	31.0	34.0
3	32.7670607667565	34.0	31.0	34.0	31.0	34.0
4	36.21216204468399	37.0	37.0	37.0	35.0	37.0
5	36.14370164304964	37.0	35.0	37.0	35.0	37.0
6	36.22150743284361	37.0	36.0	37.0	35.0	37.0
7	36.20990176475702	37.0	36.0	37.0	35.0	37.0
8	36.1770842388942	37.0	36.0	37.0	35.0	37.0
9	38.02481961227506	39.0	38.0	39.0	35.0	39.0
10	37.93940711118839	39.0	38.0	39.0	35.0	39.0
11	38.028036164478834	39.0	38.0	39.0	35.0	39.0
12	37.915239502738416	39.0	38.0	39.0	35.0	39.0
13	37.987264191949926	39.0	38.0	39.0	35.0	39.0
14	39.341867338955055	40.0	39.0	41.0	36.0	41.0
15	39.355733286968615	40.0	39.0	41.0	36.0	41.0
16	39.30074763105277	40.0	39.0	41.0	36.0	41.0
17	39.25936712162045	40.0	39.0	41.0	36.0	41.0
18	39.319481874293665	40.0	39.0	41.0	36.0	41.0
19	39.33617317221594	40.0	39.0	41.0	36.0	41.0
20	39.35069112405459	40.0	39.0	41.0	36.0	41.0
21	39.30639833087021	40.0	39.0	41.0	36.0	41.0
22	39.25628097018169	40.0	39.0	41.0	36.0	41.0
23	39.19725289055029	40.0	39.0	41.0	36.0	41.0
24	39.22450665043902	40.0	39.0	41.0	36.0	41.0
25	39.16321829088064	40.0	39.0	41.0	36.0	41.0
26	39.08554290185169	40.0	39.0	41.0	36.0	41.0
27	38.966878205685475	40.0	39.0	41.0	35.0	41.0
28	38.953751195340345	40.0	39.0	41.0	35.0	41.0
29	38.94992610623316	40.0	38.0	41.0	35.0	41.0
30	38.907545857602365	40.0	38.0	41.0	35.0	41.0
31	38.81717812744501	40.0	38.0	41.0	35.0	41.0
32	38.78118751630009	40.0	38.0	41.0	35.0	41.0
33	38.70698948100495	40.0	38.0	41.0	35.0	41.0
34	38.68338694253673	40.0	38.0	41.0	35.0	41.0
35	38.61244892636704	40.0	38.0	41.0	35.0	41.0
36	38.55029122837521	40.0	38.0	41.0	35.0	41.0
37	38.49421889941755	40.0	38.0	41.0	35.0	41.0
38	38.47109449708772	40.0	38.0	41.0	34.0	41.0
39	38.40363383465183	40.0	38.0	41.0	34.0	41.0
40	38.068808137007736	40.0	37.0	41.0	33.0	41.0
41	38.14474484916978	40.0	37.0	41.0	34.0	41.0
42	38.130139963487785	40.0	37.0	41.0	34.0	41.0
43	37.648178735981915	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	0.0
14	1.0
15	0.0
16	2.0
17	1.0
18	0.0
19	2.0
20	0.0
21	1.0
22	3.0
23	5.0
24	12.0
25	23.0
26	33.0
27	63.0
28	82.0
29	123.0
30	166.0
31	228.0
32	307.0
33	400.0
34	638.0
35	943.0
36	1364.0
37	2435.0
38	5560.0
39	10612.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.523950273841606	16.878205685473354	13.413892028166567	28.183952012518475
2	21.372685386420933	19.507954446666087	32.34808310875424	26.771277058158745
3	20.416413109623576	19.59923498217856	30.722420238198733	29.26193166999913
4	15.16126227940537	16.991219681822134	35.83413022689733	32.01338781187516
5	16.12622794053725	32.92184647483265	34.89089802660176	16.06102755802834
6	31.287490219942626	34.895244718769014	16.97817960532035	16.839085455968007
7	28.53168738589933	27.931843866817353	21.4204990002608	22.115969747022515
8	26.44962183778145	30.32252455881074	22.272450665043902	20.955402938363903
9	28.479527079892204	12.479353212205512	18.61253586021038	40.428583847691904
10	19.925236894723117	23.120055637659743	29.23150482482831	27.723202642788834
11	38.85942797531079	19.229766147961403	18.234373641658696	23.67643223506911
12	21.342258541250107	25.089107189428844	26.910371207511087	26.65826306180996
13	35.703729461879504	17.917065113448665	20.394679648787275	25.984525775884553
14	23.993740763279146	21.64652699295836	23.806833000086932	30.55289924367556
15	30.918021385725464	23.76336607841433	20.094757889246285	25.223854646613926
16	24.20672867947492	24.40667651916891	23.007041641310963	28.379553160045205
17	26.32356776493089	24.3197426758237	21.442232461097106	27.91445709814831
18	25.86716508736851	21.463965921933408	23.737285925410763	28.931583065287313
19	27.54933495609841	22.455011736068855	24.284969138485614	25.710684169347125
20	31.165782839259325	20.159958271755194	23.3460836303573	25.328175258628182
21	27.47544118925498	22.42458489089803	21.650873685125617	28.449100234721374
22	28.45344692688864	23.07224202381987	20.97713639920021	27.49717465009128
23	27.875336868642965	22.94184125880205	22.324610971051033	26.858210901503952
24	27.7492827957924	21.442232461097106	23.328696861688254	27.479787881422236
25	27.449361036251414	22.689733113100928	23.898113535599407	25.96279231504825
26	27.575415109101975	23.33739024602278	22.44631835173433	26.64087629314092
27	25.85412501086673	22.26375728070938	25.706337477179865	26.175780231244023
28	25.50638963748587	23.293923324350168	22.1290098235243	29.070677214639662
29	27.20159958271755	23.98070068677736	22.607145961922974	26.210553768582106
30	26.106233156567853	22.81144049378423	23.941580457272018	27.1407458923759
31	27.92749717465009	23.254803094844824	23.137442406328784	25.680257324176303
32	25.354255411631748	22.528905502912284	22.772320264278882	29.344518821177086
33	24.928279579240197	21.650873685125617	26.1453533860732	27.275493349560985
34	25.62809701816917	22.102929670520734	24.306702599321916	27.96227071198818
35	24.632704511866468	24.815265582891417	25.302095105624623	25.249934799617492
36	25.423802486307917	23.59384508389116	25.284708336955575	25.697644092845344
37	26.362687994436236	24.02851430061723	24.26758236981657	25.34121533512997
38	25.641137094670956	22.57671911675215	25.345562027297223	26.436581761279665
39	25.36294879596627	21.151004085890637	26.406154916108843	27.07989220203425
40	24.558810745023038	23.450404242371555	26.76693036599148	25.223854646613926
41	22.6158393462575	21.581326610449448	27.62322872294184	28.179605320351214
42	21.068416934712683	22.428931583065285	29.50099973919847	27.001651743023558
43	20.92062940102582	23.22872294184126	28.644701382248112	27.205946274884813
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.5
4	3.0
5	3.0
6	2.5
7	2.0
8	2.0
9	2.0
10	3.5
11	5.0
12	5.0
13	12.0
14	19.0
15	37.0
16	55.0
17	51.0
18	47.0
19	47.0
20	56.0
21	65.0
22	53.0
23	41.0
24	38.5
25	36.0
26	36.0
27	42.0
28	48.0
29	61.5
30	75.0
31	97.0
32	119.0
33	119.0
34	176.5
35	234.0
36	271.0
37	308.0
38	371.0
39	434.0
40	434.0
41	521.5
42	609.0
43	793.0
44	977.0
45	1508.5
46	2040.0
47	2040.0
48	2325.0
49	2610.0
50	2853.5
51	3097.0
52	3023.0
53	2949.0
54	2949.0
55	2627.0
56	2305.0
57	2197.5
58	2090.0
59	1894.0
60	1698.0
61	1698.0
62	1505.0
63	1312.0
64	1004.0
65	696.0
66	582.5
67	469.0
68	469.0
69	381.0
70	293.0
71	235.5
72	178.0
73	126.0
74	74.0
75	74.0
76	58.5
77	43.0
78	38.0
79	33.0
80	26.0
81	19.0
82	19.0
83	14.0
84	9.0
85	7.5
86	6.0
87	4.5
88	3.0
89	3.0
90	3.0
91	3.0
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	23006.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.52742762757541
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.52843518191452	52.00817178127445
2	7.0646414694454265	8.69338433452143
3	2.8682444365948427	5.29427105972355
4	1.5047686329918757	3.703381726506129
5	0.8618862592723419	2.6514822220290357
6	0.5934298834334157	2.1907328522994
7	0.4874602613917344	2.099452316786925
8	0.3037795831861533	1.4952621055376858
9	0.19074531967502648	1.0562461966443537
>10	1.4977039915224302	16.547857080761542
>50	0.09184033910279053	3.7903155698513427
>100	0.007064641469445426	0.46944275406415714
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	108	0.46944275406415714	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	91	0.39554898722072507	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	89	0.3868556028862036	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	74	0.3216552203772929	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	71	0.30861514387551076	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	69	0.2999217595409893	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	68	0.29557506737372863	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	64	0.27818829870468575	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	62	0.26949491437016426	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	61	0.26514822220290357	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	61	0.26514822220290357	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	59	0.25645483786838214	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	52	0.22602799269755713	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	51	0.22168130053029644	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	48	0.20864122402851432	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	47	0.20429453186125357	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	44	0.19125445535947144	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	42	0.18256107102495	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	41	0.1782143788576893	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	41	0.1782143788576893	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	40	0.17386768669042857	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	40	0.17386768669042857	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	39	0.16952099452316788	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	37	0.16082761018864644	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	37	0.16082761018864644	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	36	0.15648091802138572	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG	35	0.152134225854125	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	35	0.152134225854125	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	35	0.152134225854125	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	35	0.152134225854125	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	34	0.14778753368686431	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	33	0.1434408415196036	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	33	0.1434408415196036	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	32	0.13909414935234288	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	31	0.13474745718508213	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	31	0.13474745718508213	No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	31	0.13474745718508213	RNA PCR Primer, Index 17 (95% over 21bp)
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	30	0.13040076501782144	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	30	0.13040076501782144	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	30	0.13040076501782144	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	29	0.12605407285056072	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	29	0.12605407285056072	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	29	0.12605407285056072	No Hit
CATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACT	29	0.12605407285056072	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	28	0.1217073806833	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	27	0.11736068851603929	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	27	0.11736068851603929	No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG	27	0.11736068851603929	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	27	0.11736068851603929	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	27	0.11736068851603929	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	27	0.11736068851603929	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	26	0.11301399634877857	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG	26	0.11301399634877857	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	25	0.10866730418151788	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	24	0.10432061201425716	No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG	24	0.10432061201425716	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.004346692167260715	0.0
22	0.0	0.0	0.0	0.00869338433452143	0.0
23	0.0	0.0	0.0	0.00869338433452143	0.0
24	0.0	0.0	0.0	0.00869338433452143	0.0
25	0.0	0.0	0.0	0.013040076501782143	0.0
26	0.0	0.0	0.0	0.01738676866904286	0.0
27	0.0	0.0	0.0	0.021733460836303575	0.0
28	0.0	0.0	0.0	0.0869338433452143	0.0
29	0.0	0.0	0.0	0.2042945318612536	0.0
30	0.0	0.0	0.0	0.42597583239155	0.0
31	0.0	0.0	0.0	0.9084586629574893	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	50	6.692062E-9	33.300003	1
GTATCAA	70	1.7585444E-7	23.785715	2
ATCAACG	95	3.3063225E-6	17.526316	4
TATCAAC	110	7.0246097E-7	16.818182	3
TCAACGC	100	5.391399E-6	16.650002	5
CAGAGTA	100	5.391399E-6	16.650002	11
AGAGTAC	100	5.391399E-6	16.650002	12
CAACGCA	100	5.391399E-6	16.650002	6
AACGCAG	105	8.57411E-6	15.857142	7
GCAGAGT	105	8.57411E-6	15.857142	10
AGTACGG	95	6.591716E-5	15.578948	14
GAGTACG	95	6.591716E-5	15.578948	13
ACGCAGA	110	1.3329649E-5	15.136364	8
CGCAGAG	110	1.3329649E-5	15.136364	9
CTGTCTC	195	1.2403507E-7	12.333334	37
GTACGGG	115	0.004959218	11.26087	15
>>END_MODULE