FastQCFastQC Report
Fri 10 Feb 2017
ERR1631772.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631772.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378383
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA10340.27326809079689096No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA10290.2719466783655714No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9340.24683984217049923No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8850.23389000034356724No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA8650.22860435061828888No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC8270.21856161614026No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8270.21856161614026No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7680.20296894945068886No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6860.1812977855770476No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA6710.17733354828308884No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6670.17627641833803315No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT6450.17046220364022696No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6130.1620051640797816No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6120.16174088159351768No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6090.16094803413472591No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6080.16068375164846202No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6040.15962662170340633No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5360.14165541263745993No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4860.12844128832426405No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4700.12421276854404137No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4700.12421276854404137No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA4680.12368420357151352No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4630.12236279114019392No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA4630.12236279114019392No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4460.11786998887370734No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4410.11654857644238775No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4350.11496288152480422No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC4320.11417003406601248No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4300.11364146909348466No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA4280.11311290412095681No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC4140.10941294931326197No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4060.1072986894231506No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT4060.1072986894231506No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3980.10518442953303926No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3910.10333445212919186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6750.030.4222221
CATTGTA250.00549174129.62
GTCCTAC250.00549174129.61
ATTACCT250.00549174129.633
GTTGTAC250.00549174129.61
GTATGTA250.00549174129.61
TAAATAG250.00549174129.620
CCTTATC405.929762E-527.752
TAAACCG405.929762E-527.755
CCCTTAT405.929762E-527.751
TTAGATA556.243099E-726.909094
CATTATA556.243099E-726.909092
CTTAAAC358.8583556E-426.4285723
CTTATCC358.8583556E-426.4285723
TATAATA358.8583556E-426.4285725
TATCCTT358.8583556E-426.4285725
TAGATAC601.3334484E-624.6666685
AGCGGTC451.3209213E-424.66666619
GCTTAGG451.3209213E-424.6666661
GGGCAGT1550.023.8709687
Produced by FastQC (version 0.11.5)