FastQCFastQC Report
Fri 10 Feb 2017
ERR1631771.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631771.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202409
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5320.2628341625125365No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4870.24060194951805502No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4770.2356614577415036No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4260.21046494968109125No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4210.20799470379281554No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4130.2040423103715744No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4060.2005839661279884No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3930.1941613268184715No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3500.17291721217930034No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3500.17291721217930034No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3490.17242316300164517No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3370.16649457286978347No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3280.16204813027088716No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3210.15858978602730117No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2900.1432742615199917No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2530.12499444194675138No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2490.1230182452361308No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC2480.12252419605847567No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2480.12252419605847567No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2460.12153609770316537No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2440.1205479993478551No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2420.1195599009925448No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2300.11363131086068307No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2270.11214916332771764No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC2250.11116106497240734No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2240.11066701579475222No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2210.10918486826178676No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2200.10869081908413164No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2190.10819676990647649No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2120.10473842566289049No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2080.10276222895226991No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2050.10128008141930447No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA2040.10078603224164934No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGC303.5891004E-430.83333411
AAATCTC303.5891004E-430.8333349
GGTATCA2950.030.1016941
ACACGAA250.00548578229.617
TTAAGTG250.00548578229.616
CCATAGT250.00548578229.62
AAACACG250.00548578229.615
GAGAAAT405.916346E-527.75000216
GAAATCT358.8440644E-426.4285728
GGGAAAT358.8440644E-426.4285726
GAAACGA358.8440644E-426.42857224
AGTAGGC358.8440644E-426.4285726
ATACACA601.3283316E-624.66666837
AAATCCA400.001925724223.12500219
CTTGGAC400.001925724223.1250023
TCTAAGT802.6977432E-823.12500233
GTCCCAA400.001925724223.1250021
ATCATTC400.001925724223.12500227
CTGTGCA400.001925724223.1250029
GGAGAAA502.691458E-422.215
Produced by FastQC (version 0.11.5)