##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631771.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	202409
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40545627911802	33.0	31.0	34.0	31.0	34.0
2	32.583531364712044	34.0	31.0	34.0	31.0	34.0
3	32.663794594113895	34.0	31.0	34.0	31.0	34.0
4	36.15245369524082	37.0	35.0	37.0	35.0	37.0
5	36.04458793828338	37.0	35.0	37.0	35.0	37.0
6	36.09335553260971	37.0	35.0	37.0	35.0	37.0
7	36.09889876438301	37.0	35.0	37.0	35.0	37.0
8	36.06572830259524	37.0	35.0	37.0	35.0	37.0
9	37.88531142389913	39.0	38.0	39.0	35.0	39.0
10	37.792622857679255	39.0	37.0	39.0	35.0	39.0
11	37.86752071301177	39.0	38.0	39.0	35.0	39.0
12	37.80316092663864	39.0	38.0	39.0	35.0	39.0
13	37.8595714617433	39.0	38.0	39.0	35.0	39.0
14	39.1750663261021	40.0	39.0	41.0	36.0	41.0
15	39.19045595798606	40.0	39.0	41.0	36.0	41.0
16	39.123714854576626	40.0	38.0	41.0	36.0	41.0
17	39.09199195687939	40.0	38.0	41.0	36.0	41.0
18	39.12657045882347	40.0	39.0	41.0	36.0	41.0
19	39.13885252137998	40.0	39.0	41.0	36.0	41.0
20	39.13898097416617	40.0	39.0	41.0	36.0	41.0
21	39.09543053915587	40.0	39.0	41.0	36.0	41.0
22	39.063687879491525	40.0	39.0	41.0	36.0	41.0
23	39.00036559639147	40.0	39.0	41.0	36.0	41.0
24	39.01098271321927	40.0	38.0	41.0	35.0	41.0
25	38.948658409458076	40.0	38.0	41.0	35.0	41.0
26	38.876784135092805	40.0	38.0	41.0	35.0	41.0
27	38.760475077689236	40.0	38.0	41.0	35.0	41.0
28	38.76469919815818	40.0	38.0	41.0	35.0	41.0
29	38.74270412876898	40.0	38.0	41.0	35.0	41.0
30	38.69415391608081	40.0	38.0	41.0	35.0	41.0
31	38.60074403806155	40.0	38.0	41.0	35.0	41.0
32	38.563843504982486	40.0	38.0	41.0	35.0	41.0
33	38.48410396770895	40.0	38.0	41.0	34.0	41.0
34	38.46126901471772	40.0	38.0	41.0	34.0	41.0
35	38.3927147508263	40.0	38.0	41.0	34.0	41.0
36	38.346575498125084	40.0	38.0	41.0	34.0	41.0
37	38.29563902790884	40.0	38.0	41.0	34.0	41.0
38	38.28233922404636	40.0	38.0	41.0	34.0	41.0
39	38.207648869368455	40.0	38.0	41.0	34.0	41.0
40	37.88654654684327	40.0	37.0	41.0	33.0	41.0
41	37.965021318222014	40.0	37.0	41.0	33.0	41.0
42	37.95555039548637	40.0	37.0	41.0	33.0	41.0
43	37.44742081626805	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	7.0
20	11.0
21	20.0
22	42.0
23	70.0
24	132.0
25	211.0
26	359.0
27	557.0
28	798.0
29	1196.0
30	1632.0
31	2304.0
32	3010.0
33	4085.0
34	5924.0
35	9013.0
36	13613.0
37	24564.0
38	56063.0
39	78796.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.26294285333162	16.717636073494756	13.471238927122805	27.54818214605082
2	20.804410871058103	19.924015236476638	32.58995400402156	26.6816198884437
3	20.862708674021412	20.163629087639386	29.94382660850061	29.029835629838598
4	15.611954013902544	16.643528696846484	34.162512536497886	33.582004752753086
5	16.52100450078801	33.12945570602098	33.75887435835363	16.590665434837383
6	33.130443804376284	34.056786012479684	16.13959853563824	16.673171647505793
7	29.644432806841593	28.021975307422103	20.43683828288268	21.89675360285363
8	26.712744986635972	31.26540815872812	20.904208804944442	21.117638049691468
9	28.1178208478872	13.00189220835042	18.065896279315645	40.814390664446734
10	19.32769787904688	23.87640865771779	29.82970124846227	26.96619221477306
11	38.61340157799307	19.707621696663686	18.909732274750628	22.769244450592613
12	22.30039178099788	24.259790819578182	26.89603723154603	26.543780167877912
13	35.039449826835764	17.9320089521711	21.313775573220557	25.71476564777258
14	23.924825477127992	20.640880593254252	23.790444100805793	31.64384982881196
15	29.53475388940215	24.46531527748272	20.406701283045713	25.593229550069413
16	24.820042587039115	24.376386425504794	22.926846138264604	27.876724849191486
17	26.520065807350463	24.078474771378744	22.00050393016121	27.400955491109585
18	26.323434234643717	21.755949587221913	23.458443053421536	28.462173124712837
19	27.896980865475353	22.82655415520061	23.498461036811605	25.77800394251244
20	29.953707592053714	20.55788033140819	23.44707992233547	26.041332154202628
21	27.168258328434014	22.487142370151524	22.292486994155396	28.052112307259065
22	28.07385047107589	23.274656759333823	21.437781916812	27.213710852778284
23	27.55312263782737	22.500975747125867	22.651660746310686	27.294240868736075
24	27.294240868736075	21.960979995948797	23.423365561808023	27.321413573507108
25	27.68157542401771	22.197629552045612	23.483145512304297	26.63764951163239
26	27.468640228448336	23.186716005711208	22.678339401904065	26.666304363936387
27	26.50623243037612	22.116605486910167	24.699988636868913	26.677173445844797
28	25.944004466204568	23.249954300451066	22.293475092510707	28.512566140833655
29	26.725590265255008	23.768211887811315	22.57211882870821	26.934079018225475
30	26.52253605323874	22.738613401577993	23.906051608377098	26.832798936806167
31	27.765563784219083	22.571624779530556	23.046406039257146	26.616405396993216
32	25.419324239534802	22.560261648444484	23.121995563438386	28.898418548582328
33	25.41438374775825	22.246046371455815	25.267651141994673	27.07191873879126
34	25.717235893660856	22.011867061247276	24.43171993340217	27.8391771116897
35	25.24047843722364	23.813664412155585	25.302234584430533	25.64362256619024
36	25.59026525500348	22.730708614735512	25.371401469302253	26.30762466095875
37	26.271065021812273	22.626958287427932	24.819548537861458	26.28242815289834
38	25.160936519621163	22.02570043822162	25.72514068050334	27.08822236165388
39	24.890197570266146	20.7317856419428	26.910364657698025	27.46765213009303
40	24.278564688329077	21.94467637308618	27.622783571876745	26.153975366708003
41	22.864595941880054	20.839982411849274	28.778858647589782	27.51656299868089
42	21.746562652846464	21.04402472222085	29.62022439713649	27.589188227796196
43	20.701648642105834	22.07955179858603	29.289211448107544	27.929588111200587
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	2.0
2	2.0
3	6.5
4	11.0
5	11.0
6	15.0
7	19.0
8	21.5
9	24.0
10	37.5
11	51.0
12	51.0
13	96.5
14	142.0
15	272.0
16	402.0
17	381.5
18	361.0
19	361.0
20	460.5
21	560.0
22	481.5
23	403.0
24	461.5
25	520.0
26	520.0
27	596.5
28	673.0
29	931.0
30	1189.0
31	1418.0
32	1647.0
33	1647.0
34	2067.0
35	2487.0
36	2796.5
37	3106.0
38	3864.0
39	4622.0
40	4622.0
41	5542.0
42	6462.0
43	7590.5
44	8719.0
45	12511.0
46	16303.0
47	16303.0
48	18234.0
49	20165.0
50	22486.5
51	24808.0
52	24977.0
53	25146.0
54	25146.0
55	22673.0
56	20200.0
57	19029.5
58	17859.0
59	15889.5
60	13920.0
61	13920.0
62	12500.0
63	11080.0
64	9164.5
65	7249.0
66	6185.5
67	5122.0
68	5122.0
69	4303.0
70	3484.0
71	2912.0
72	2340.0
73	1852.0
74	1364.0
75	1364.0
76	1093.5
77	823.0
78	683.5
79	544.0
80	408.5
81	273.0
82	273.0
83	226.0
84	179.0
85	132.5
86	86.0
87	56.5
88	27.0
89	27.0
90	25.5
91	24.0
92	17.5
93	11.0
94	5.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	202409.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.378290784631645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.52391075064958	44.79596266336407
2	6.465583855830196	6.773124625861876
3	2.296596380298646	3.608753790666449
4	1.2530671498574408	2.625340621916105
5	0.7762905784983495	2.0330386826978226
6	0.5854533061243085	1.8399026105401797
7	0.41161467036314614	1.509177102685082
8	0.34867349597671865	1.4610337408930127
9	0.2777399104194967	1.309278761140495
>10	1.795318765824084	17.797190437801742
>50	0.16284750072842252	5.916872457299397
>100	0.10190457100796992	10.051932699413117
>500	9.99064421646764E-4	0.27839180572064026
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	532	0.2628341625125365	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	487	0.24060194951805502	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	477	0.2356614577415036	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	426	0.21046494968109125	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	421	0.20799470379281554	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	413	0.2040423103715744	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	406	0.2005839661279884	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	393	0.1941613268184715	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	350	0.17291721217930034	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	350	0.17291721217930034	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	349	0.17242316300164517	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	337	0.16649457286978347	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	328	0.16204813027088716	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	321	0.15858978602730117	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	290	0.1432742615199917	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	253	0.12499444194675138	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	249	0.1230182452361308	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	248	0.12252419605847567	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	248	0.12252419605847567	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	246	0.12153609770316537	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	244	0.1205479993478551	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	242	0.1195599009925448	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	230	0.11363131086068307	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	227	0.11214916332771764	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	225	0.11116106497240734	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	224	0.11066701579475222	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	221	0.10918486826178676	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	220	0.10869081908413164	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	219	0.10819676990647649	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	212	0.10473842566289049	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	208	0.10276222895226991	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	205	0.10128008141930447	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	204	0.10078603224164934	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	4.940491776551438E-4	0.0
14	0.0	0.0	0.0	4.940491776551438E-4	0.0
15	0.0	0.0	0.0	4.940491776551438E-4	0.0
16	0.0	0.0	0.0	4.940491776551438E-4	0.0
17	0.0	0.0	0.0	4.940491776551438E-4	0.0
18	4.940491776551438E-4	0.0	0.0	9.880983553102877E-4	0.0
19	4.940491776551438E-4	0.0	0.0	0.0019761967106205753	0.0
20	4.940491776551438E-4	0.0	0.0	0.002964295065930863	0.0
21	4.940491776551438E-4	0.0	0.0	0.0034583442435860067	0.0
22	4.940491776551438E-4	0.0	0.0	0.004940491776551438	0.0
23	4.940491776551438E-4	0.0	0.0	0.005434540954206582	0.0
24	4.940491776551438E-4	0.0	0.0	0.009386934375447731	0.0
25	4.940491776551438E-4	0.0	0.0	0.010869081908413163	0.0
26	4.940491776551438E-4	0.0	0.0	0.01432742615199917	0.0
27	4.940491776551438E-4	0.0	0.0	0.031125098192274058	0.0
28	4.940491776551438E-4	0.0	0.0	0.1284527861903374	0.0
29	9.880983553102877E-4	0.0	0.0	0.2796318345528114	0.0
30	9.880983553102877E-4	0.0	0.0	0.48663843999031664	0.0
31	9.880983553102877E-4	0.0	0.0	1.0454080599182842	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGC	30	3.5891004E-4	30.833334	11
AAATCTC	30	3.5891004E-4	30.833334	9
GGTATCA	295	0.0	30.101694	1
ACACGAA	25	0.005485782	29.6	17
TTAAGTG	25	0.005485782	29.6	16
CCATAGT	25	0.005485782	29.6	2
AAACACG	25	0.005485782	29.6	15
GAGAAAT	40	5.916346E-5	27.750002	16
GAAATCT	35	8.8440644E-4	26.428572	8
GGGAAAT	35	8.8440644E-4	26.428572	6
GAAACGA	35	8.8440644E-4	26.428572	24
AGTAGGC	35	8.8440644E-4	26.428572	6
ATACACA	60	1.3283316E-6	24.666668	37
AAATCCA	40	0.0019257242	23.125002	19
CTTGGAC	40	0.0019257242	23.125002	3
TCTAAGT	80	2.6977432E-8	23.125002	33
GTCCCAA	40	0.0019257242	23.125002	1
ATCATTC	40	0.0019257242	23.125002	27
CTGTGCA	40	0.0019257242	23.125002	9
GGAGAAA	50	2.691458E-4	22.2	15
>>END_MODULE