FastQCFastQC Report
Fri 10 Feb 2017
ERR1631770.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631770.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832071
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA20550.24697411639150987No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA19240.2312302676093747No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA18510.22245697783001692No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA18220.21897169832862823No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC16700.2007040264592805No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG16340.19637747259548763No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA16210.19481510592245133No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT15480.18604181614309356No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA13710.16476959297944527No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC13600.163447590409953No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT13310.1599623109085643No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12810.15395320831996306No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA12620.15166974933629462No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10310.12390769537695702No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10260.12330678511809688No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA9570.11501422354582723No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA9320.11200967225152661No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8890.10684184402532956No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8690.10443820298988908No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8450.10155383374736052No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8390.10083274143672837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13850.029.5198551
ATACACA1600.024.2812537
ATACGGC1850.021.029
GCGGTAA7550.020.3377523
CAATGAG4450.019.95505733
TATAGAC656.899203E-519.9230772
TATACAC3450.019.84057837
CCGCTTA851.2444634E-619.58823625
TCCTAAA851.2444634E-619.5882362
GTATCAA20800.019.5673082
TGCGGGT5700.019.47368421
TACGGCT2000.019.42500130
TAGCGAA1052.257184E-819.3809510
GCAGTCG2500.019.249
CGCGGTA8000.019.1937522
GTTTATA1355.638867E-1119.1851841
AATGAGC4550.019.1098934
TCTATGG3650.018.7534242
CTGCGGG5950.018.65546220
GGTAACG6000.018.525
Produced by FastQC (version 0.11.5)