FastQCFastQC Report
Fri 10 Feb 2017
ERR1631769.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631769.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87862
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2190.24925451275864424No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2190.24925451275864424No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2020.22990598893719696No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1670.1900707928342173No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1630.18551819899387678No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1590.18096560515353624No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1570.17868930823336596No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1520.1729985659329403No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1440.16389337825225922No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1400.15934078441191868No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1290.14682115135098223No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1200.13657781521021603No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1150.13088707290979035No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1130.1286107759896201No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1130.1286107759896201No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1110.1263344790694498No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1080.12292003368919442No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.12178188522910928No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1060.12064373676902414No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT940.10698595524800256No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG920.10470965832783227No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC910.10357150986774716No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT900.10243336140766202No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTCA251.2243683E-436.99999622
GCAGTCG303.5727202E-430.8333349
GGTATCA1800.030.8333341
GGAGCGA250.005469097329.59999820
GTTCTAG250.005469097329.5999981
TTCAACG250.005469097329.59999819
ACGATTA250.005469097329.59999823
TTCCATG250.005469097329.5999981
GGTGGTA250.005469097329.59999818
AGGTTCG250.005469097329.5999988
ACGTGAT250.005469097329.59999837
TACGTGA250.005469097329.59999836
TCAATGA453.9531405E-628.77777932
CATAGGT358.8040845E-426.4285716
CAGTCGG358.8040845E-426.4285710
GATTCCT358.8040845E-426.4285718
TGAGCAT358.8040845E-426.428576
GGTAACG508.966956E-625.89999825
GTCAATG508.966956E-625.89999831
GTAACGT508.966956E-625.89999826
Produced by FastQC (version 0.11.5)