##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631769.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	87862
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.608181011131094	34.0	31.0	34.0	31.0	34.0
2	32.77608067196285	34.0	31.0	34.0	31.0	34.0
3	32.86351323666659	34.0	31.0	34.0	31.0	34.0
4	36.29078555006715	37.0	37.0	37.0	35.0	37.0
5	36.21320935102775	37.0	35.0	37.0	35.0	37.0
6	36.2700940110628	37.0	37.0	37.0	35.0	37.0
7	36.249493523935264	37.0	37.0	37.0	35.0	37.0
8	36.22368031686053	37.0	37.0	37.0	35.0	37.0
9	38.05936582367804	39.0	38.0	39.0	35.0	39.0
10	37.989893241674444	39.0	38.0	39.0	35.0	39.0
11	38.07544786141904	39.0	38.0	39.0	37.0	39.0
12	38.00868407275045	39.0	38.0	39.0	35.0	39.0
13	38.0681864742437	39.0	38.0	39.0	37.0	39.0
14	39.44960278618743	40.0	39.0	41.0	37.0	41.0
15	39.44864674148096	40.0	39.0	41.0	37.0	41.0
16	39.388142769342835	40.0	39.0	41.0	37.0	41.0
17	39.36611959664019	40.0	39.0	41.0	36.0	41.0
18	39.38914433998771	40.0	39.0	41.0	37.0	41.0
19	39.40076483576518	40.0	39.0	41.0	37.0	41.0
20	39.407263663472264	40.0	39.0	41.0	37.0	41.0
21	39.366142359609384	40.0	39.0	41.0	36.0	41.0
22	39.34384603127632	40.0	39.0	41.0	36.0	41.0
23	39.28103161776422	40.0	39.0	41.0	36.0	41.0
24	39.28702966014887	40.0	39.0	41.0	36.0	41.0
25	39.259122259907585	40.0	39.0	41.0	36.0	41.0
26	39.188352188659486	40.0	39.0	41.0	36.0	41.0
27	39.06752634813685	40.0	39.0	41.0	35.0	41.0
28	39.075038127973414	40.0	39.0	41.0	35.0	41.0
29	39.05960483485466	40.0	39.0	41.0	35.0	41.0
30	38.998042384648656	40.0	39.0	41.0	35.0	41.0
31	38.9345223190913	40.0	38.0	41.0	35.0	41.0
32	38.90099246545719	40.0	38.0	41.0	35.0	41.0
33	38.83380756185837	40.0	38.0	41.0	35.0	41.0
34	38.7901026609911	40.0	38.0	41.0	35.0	41.0
35	38.71097858004598	40.0	38.0	41.0	35.0	41.0
36	38.662470692677154	40.0	38.0	41.0	35.0	41.0
37	38.62911156131206	40.0	38.0	41.0	35.0	41.0
38	38.584029500808086	40.0	38.0	41.0	35.0	41.0
39	38.524253943684414	40.0	38.0	41.0	35.0	41.0
40	38.17699346702784	40.0	38.0	41.0	34.0	41.0
41	38.279859324850335	40.0	37.0	41.0	34.0	41.0
42	38.277344016753545	40.0	37.0	41.0	34.0	41.0
43	37.81920511711547	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	8.0
21	8.0
22	11.0
23	27.0
24	39.0
25	75.0
26	121.0
27	166.0
28	269.0
29	402.0
30	580.0
31	810.0
32	1124.0
33	1543.0
34	2234.0
35	3251.0
36	5056.0
37	9114.0
38	20673.0
39	42349.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.71812615237531	16.092281077143703	12.911156131205754	28.278436639275228
2	20.87819535180169	19.32689900070565	32.39853406478341	27.39637158270925
3	20.819011631877263	19.551114247342426	29.259520611868613	30.370353508911702
4	15.347931984248026	15.905624729689741	34.053401925747195	34.69304136031504
5	16.868498326921763	32.81851084655483	33.450183241902074	16.86280758462134
6	33.85877853907264	33.97486968200132	15.714415788395439	16.451935990530604
7	30.357833875850766	27.818624661400833	19.86524322232592	21.95829824042248
8	27.324668229723887	30.563838747126177	20.44569893696934	21.6657940861806
9	28.35469258610093	12.468416380232638	17.585531856775397	41.59135917689103
10	19.17097266167399	23.53918645148073	29.552024766110492	27.737816120734788
11	39.23880630989506	19.300721586123696	18.659944003095763	22.80052810088548
12	22.745896974801393	23.81575652728142	26.704377318977485	26.7339691789397
13	35.41007489016867	17.351073273997862	20.963556486308075	26.27529534952539
14	23.92501877944959	20.05189956977988	23.34797751018643	32.6751041405841
15	29.86843003801416	24.063872891579976	19.98474881063486	26.082948259771005
16	25.511597732808266	24.263048872094874	22.312262411508957	27.913090983587903
17	27.19036671143384	23.746329471216228	21.44613143338417	27.617172383965766
18	26.575766542987868	21.21963988982723	23.78957911269946	28.415014454485444
19	28.280712936195396	22.05959345337006	23.46179235619494	26.197901254239603
20	30.190526052218253	20.51512599303453	22.817600327786757	26.47674762696046
21	27.336049714324734	22.130158657895336	21.970817873483416	28.56297375429651
22	28.78946529785345	22.841501445448547	21.056884660035056	27.31214859666295
23	27.963169515831645	22.05504085952972	22.30429537228836	27.677494252350275
24	28.0280439780565	21.48824292640732	22.933691470715438	27.550021624820744
25	28.206733286289865	21.61912999931711	23.067992989005486	27.10614372538754
26	27.727572784594024	22.663950285675263	22.58086544808905	27.027611481641667
27	27.034440372402173	21.43247365186315	24.375725569643304	27.157360406091367
28	26.09546789283194	23.189774874234594	22.19161867473993	28.52313855819353
29	27.23361635291707	23.45268716851426	22.462498008240196	26.85119847032847
30	26.593976918349227	22.19730941704036	23.692836493592225	27.51587717101819
31	27.97682729735267	22.482984680521728	22.85174478158931	26.688443240536298
32	25.64362295417814	22.203000159340785	23.187498577314425	28.965878309166648
33	25.680043704900868	22.12902050943525	24.395074093464753	27.795861692199132
34	26.1136782681933	21.806924495231158	24.232318863672578	27.84707837290296
35	25.55143292891125	23.41512826933145	24.82529421137693	26.208144590380368
36	25.998725273724705	21.948054904281715	25.567367007352438	26.48585281464114
37	26.466504290819692	21.5280781225103	24.977806105028343	27.027611481641667
38	25.51842662356878	20.90095832100339	26.21952607498122	27.361088980446606
39	24.835537547517696	20.103116250483712	27.05151259930345	28.00983360269513
40	24.684163802326374	20.98176686166944	28.197628098609183	26.136441237395008
41	22.825567367007352	20.16002367348797	29.26976394800938	27.7446450114953
42	21.506453301768683	20.54357970453666	30.124513441533313	27.825453552161346
43	20.485534133072317	21.28223805513191	30.368077211991533	27.864150599804237
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	3.0
4	6.0
5	6.0
6	8.5
7	11.0
8	9.5
9	8.0
10	11.0
11	14.0
12	14.0
13	44.0
14	74.0
15	129.0
16	184.0
17	184.0
18	184.0
19	184.0
20	209.5
21	235.0
22	200.0
23	165.0
24	184.0
25	203.0
26	203.0
27	233.5
28	264.0
29	383.5
30	503.0
31	588.5
32	674.0
33	674.0
34	810.0
35	946.0
36	1097.5
37	1249.0
38	1604.0
39	1959.0
40	1959.0
41	2352.0
42	2745.0
43	3185.0
44	3625.0
45	5020.5
46	6416.0
47	6416.0
48	7210.5
49	8005.0
50	9111.5
51	10218.0
52	10403.0
53	10588.0
54	10588.0
55	9688.0
56	8788.0
57	8205.0
58	7622.0
59	7054.0
60	6486.0
61	6486.0
62	6001.5
63	5517.0
64	4527.5
65	3538.0
66	3015.0
67	2492.0
68	2492.0
69	2166.0
70	1840.0
71	1548.5
72	1257.0
73	1054.5
74	852.0
75	852.0
76	672.0
77	492.0
78	402.5
79	313.0
80	243.0
81	173.0
82	173.0
83	135.0
84	97.0
85	80.5
86	64.0
87	48.0
88	32.0
89	32.0
90	23.0
91	14.0
92	11.0
93	8.0
94	4.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	87862.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.35844847601921
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.37515269095351	55.3595410985409
2	5.6854925630523825	7.204479752338895
3	2.220306100452684	4.220254489995675
4	1.1514694258820148	2.9182126516582825
5	0.7257311202126895	2.2990598893719696
6	0.5101674211396134	1.9394049759850676
7	0.3772364733778832	1.6730782363251464
8	0.3143637278149026	1.5934078441191868
9	0.22993461234461451	1.3111470260180738
>10	1.2808076453258603	14.309940588650383
>50	0.09520730042394193	4.04839407252282
>100	0.03413091901990371	3.1230793744736065
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	219	0.24925451275864424	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	219	0.24925451275864424	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	202	0.22990598893719696	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	167	0.1900707928342173	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	163	0.18551819899387678	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	159	0.18096560515353624	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	157	0.17868930823336596	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	152	0.1729985659329403	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	144	0.16389337825225922	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	140	0.15934078441191868	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	129	0.14682115135098223	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	120	0.13657781521021603	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	115	0.13088707290979035	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	113	0.1286107759896201	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	113	0.1286107759896201	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	111	0.1263344790694498	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	108	0.12292003368919442	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	107	0.12178188522910928	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	106	0.12064373676902414	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	94	0.10698595524800256	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	92	0.10470965832783227	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	91	0.10357150986774716	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	90	0.10243336140766202	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0011381484600851335	0.0	0.0	0.0011381484600851335	0.0
19	0.0011381484600851335	0.0	0.0	0.0011381484600851335	0.0
20	0.0011381484600851335	0.0	0.0	0.0034144453802554004	0.0
21	0.0011381484600851335	0.0	0.0	0.0034144453802554004	0.0
22	0.0011381484600851335	0.0	0.0	0.009105187680681068	0.0
23	0.0011381484600851335	0.0	0.0	0.009105187680681068	0.0
24	0.0011381484600851335	0.0	0.0	0.010243336140766201	0.0
25	0.0011381484600851335	0.0	0.0	0.013657781521021602	0.0
26	0.0011381484600851335	0.0	0.0	0.01593407844119187	0.0
27	0.0011381484600851335	0.0	0.0	0.03414445380255401	0.0
28	0.0011381484600851335	0.0	0.0	0.16389337825225922	0.0
29	0.0011381484600851335	0.0	0.0	0.3220960142040928	0.0
30	0.0011381484600851335	0.0	0.0	0.5770412692631627	0.0
31	0.0011381484600851335	0.0	0.0	1.209851813070497	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTCA	25	1.2243683E-4	36.999996	22
GCAGTCG	30	3.5727202E-4	30.833334	9
GGTATCA	180	0.0	30.833334	1
GGAGCGA	25	0.0054690973	29.599998	20
GTTCTAG	25	0.0054690973	29.599998	1
TTCAACG	25	0.0054690973	29.599998	19
ACGATTA	25	0.0054690973	29.599998	23
TTCCATG	25	0.0054690973	29.599998	1
GGTGGTA	25	0.0054690973	29.599998	18
AGGTTCG	25	0.0054690973	29.599998	8
ACGTGAT	25	0.0054690973	29.599998	37
TACGTGA	25	0.0054690973	29.599998	36
TCAATGA	45	3.9531405E-6	28.777779	32
CATAGGT	35	8.8040845E-4	26.42857	16
CAGTCGG	35	8.8040845E-4	26.42857	10
GATTCCT	35	8.8040845E-4	26.42857	18
TGAGCAT	35	8.8040845E-4	26.42857	6
GGTAACG	50	8.966956E-6	25.899998	25
GTCAATG	50	8.966956E-6	25.899998	31
GTAACGT	50	8.966956E-6	25.899998	26
>>END_MODULE