FastQCFastQC Report
Fri 10 Feb 2017
ERR1631768.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631768.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63349
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1660.26204044262735005No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1460.2304693049614043No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1290.20363383794535037No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1250.1973196104121612No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1130.17837692781259373No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1000.15785568832972896No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1000.15785568832972896No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG940.1483843470299452No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC860.1357558919635669No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA820.12944166443037775No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA750.11839176624729672No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA720.11365609559740486No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT680.10734186806421571No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT670.10576331118091839No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA670.10576331118091839No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT640.10102764053102654No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGACC200.001828829337.037
TGGGTAA250.005457680729.610
CAAGCCT250.005457680729.618
ACTCAGA250.005457680729.618
GGTATCA1000.027.7500021
CGTGCCA556.106129E-726.9090910
TAATACG451.3040191E-424.66666627
GCGGTAA601.3043737E-624.66666623
GTAATAC601.3043737E-624.66666626
CTAACTC601.3043737E-624.6666663
AACTCCG601.3043737E-624.6666665
CGGTAAT601.3043737E-624.66666624
GCTAACT601.3043737E-624.6666662
TAACTCC601.3043737E-624.6666664
GGCTAAC601.3043737E-624.6666661
GTGCCAG701.8639366E-723.78571511
CTTCCTC400.00191120823.12500218
AACTGTC400.00191120823.12500236
GGTAATA652.6169891E-622.7692325
TACGGAG502.6632243E-422.230
Produced by FastQC (version 0.11.5)