##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631768.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	63349
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23465248070214	31.0	31.0	34.0	30.0	34.0
2	31.443448199655876	31.0	31.0	34.0	30.0	34.0
3	31.523039037711722	31.0	31.0	34.0	30.0	34.0
4	35.36317858214021	37.0	35.0	37.0	33.0	37.0
5	34.63107547080459	35.0	35.0	37.0	30.0	37.0
6	34.63072818829026	35.0	35.0	37.0	31.0	37.0
7	34.58905428657122	35.0	35.0	37.0	31.0	37.0
8	34.538540466305705	35.0	35.0	37.0	30.0	37.0
9	36.19788789089015	37.0	35.0	39.0	32.0	39.0
10	35.94713412997837	37.0	35.0	39.0	32.0	39.0
11	36.0349018926897	37.0	35.0	39.0	32.0	39.0
12	35.95513741337669	37.0	35.0	39.0	31.0	39.0
13	36.05747525612085	37.0	35.0	39.0	32.0	39.0
14	36.98312522691755	38.0	36.0	40.0	32.0	41.0
15	36.95690539708598	38.0	36.0	40.0	32.0	41.0
16	36.86871142401616	38.0	36.0	40.0	31.0	41.0
17	36.822364993922555	38.0	36.0	40.0	31.0	41.0
18	36.81713997063884	38.0	36.0	40.0	31.0	41.0
19	36.845853920346016	38.0	36.0	40.0	31.0	41.0
20	36.7984972138471	38.0	36.0	40.0	31.0	41.0
21	36.75944371655432	38.0	36.0	40.0	31.0	41.0
22	36.6934600388325	38.0	36.0	40.0	31.0	41.0
23	36.63710555809879	38.0	36.0	40.0	31.0	41.0
24	36.618983725078536	38.0	35.0	40.0	31.0	41.0
25	36.5226443984909	38.0	35.0	40.0	30.0	41.0
26	36.38215283587744	38.0	35.0	40.0	30.0	41.0
27	36.19439928017806	38.0	35.0	40.0	30.0	41.0
28	36.13468247328292	38.0	35.0	40.0	30.0	41.0
29	36.097981025746265	38.0	35.0	40.0	30.0	41.0
30	36.084721147926565	38.0	35.0	40.0	30.0	41.0
31	35.95084373865412	38.0	34.0	40.0	29.0	41.0
32	35.86339168731945	38.0	34.0	40.0	29.0	41.0
33	35.766294653427835	38.0	34.0	40.0	29.0	41.0
34	35.72073750177588	38.0	34.0	40.0	29.0	41.0
35	35.60930717138392	38.0	34.0	40.0	28.0	41.0
36	35.548217020000315	38.0	34.0	40.0	28.0	41.0
37	35.5126837045573	38.0	34.0	40.0	28.0	41.0
38	35.45183033670618	38.0	34.0	40.0	28.0	41.0
39	35.34494625013812	38.0	34.0	40.0	28.0	41.0
40	34.96484553820897	37.0	33.0	40.0	26.0	40.0
41	35.05341836493078	37.0	33.0	40.0	27.0	40.0
42	35.01990560229838	37.0	33.0	40.0	27.0	40.0
43	34.37383384110247	37.0	33.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	6.0
19	9.0
20	25.0
21	35.0
22	66.0
23	113.0
24	185.0
25	273.0
26	422.0
27	586.0
28	797.0
29	1205.0
30	1627.0
31	2249.0
32	2972.0
33	4204.0
34	5724.0
35	7906.0
36	10097.0
37	11540.0
38	10026.0
39	3279.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.97856319752482	17.08945681857646	13.247249364630854	27.684730619267867
2	21.07215583513552	20.410740501033956	31.77950717454103	26.73759648928949
3	21.362610301662222	20.379169363368007	29.746325908854125	28.511894426115646
4	16.79742379516646	16.263871568611975	33.44804180018627	33.4906628360353
5	16.40752024499203	33.31070735133941	33.855309476076975	16.426462927591594
6	34.417275726530804	33.945287218424916	15.258330833951602	16.379106221092677
7	29.908917267833747	28.259325324788076	20.00505138202655	21.826706025351626
8	27.444789973006678	30.944450583276765	20.532289381047846	21.078470062668707
9	27.749451451483054	13.095707903834313	17.409903865885806	41.74493677879683
10	18.96478239593364	24.303461775245072	29.863139118218125	26.868616710603167
11	39.24134556188733	19.42572100585645	18.780091240587854	22.552842191668375
12	22.699647981815023	24.150341757565233	26.150373328702898	26.999636931916843
13	34.62248812135945	17.640373170847212	21.266318331781086	26.47082037601225
14	23.79200934505675	20.050829531642172	22.972738322625457	33.18442280067562
15	29.446400101027642	24.69967955295269	19.747746610049095	26.10617373597058
16	25.711534515146255	24.224533931080206	22.281330407741244	27.7826011460323
17	27.525296374054843	23.833051824022476	21.324724936463085	27.316926865459596
18	26.363478507948034	21.58992249285703	23.395791567349132	28.650807431845806
19	28.237225528421916	22.396565060221945	23.375270327866264	25.990939083489874
20	29.703704873005098	20.818008176924653	23.11638699900551	26.36189995106474
21	27.195377985445706	22.320794329823677	22.068225228496107	28.415602456234513
22	28.39192410298505	22.91748883171005	21.17160491878325	27.518982146521648
23	27.80312238551516	22.331844228006755	22.431293311654485	27.433740074823596
24	27.853636205780674	21.94983346224881	22.565470646734756	27.63105968523576
25	28.12041231905792	22.12031760564492	22.898546149110484	26.86072392618668
26	28.096733965808458	22.985366777691834	22.167674312143838	26.75022494435587
27	27.013843943866515	21.918262324582866	24.044578446384314	27.023315285166298
28	26.118802191036956	23.30265671123459	22.424979084121297	28.153562013607157
29	27.037522297115977	23.80463780012313	22.04296831836335	27.114871584397545
30	26.789688866438304	22.72490489194778	23.280556914868427	27.20484932674549
31	28.055691486842726	22.513378269585946	22.661762616615892	26.769167626955436
32	25.337416533804795	22.436028982304375	23.30107815435129	28.925476329539535
33	26.150373328702898	22.26554483890827	24.27346919446242	27.310612637926408
34	26.404520986913766	21.994033054981134	23.80305924323983	27.79838671486527
35	25.80466937126079	23.520497561129616	24.23874094302988	26.43609212457971
36	26.374528406131116	22.34131556930654	24.784921624650742	26.499234399911604
37	26.805474435271275	22.096639252395462	24.265576410045934	26.832309902287328
38	26.013038879856037	21.545722900124705	25.42897283303604	27.01226538698322
39	25.260067246523228	20.870100554073467	26.31454324456582	27.555288954837486
40	24.95067009739696	21.599393834156814	26.85598825553679	26.59394781290944
41	23.327913621367347	20.92535004498887	28.354038737785913	27.392697595857868
42	22.546527964135187	21.277368229964168	28.497687414165966	27.67841639173468
43	21.65306476818892	21.883534073150326	28.761306413676618	27.702094744984134
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.0
6	1.5
7	1.0
8	2.5
9	4.0
10	4.5
11	5.0
12	5.0
13	20.0
14	35.0
15	60.5
16	86.0
17	86.0
18	86.0
19	86.0
20	98.0
21	110.0
22	109.0
23	108.0
24	118.0
25	128.0
26	128.0
27	149.5
28	171.0
29	269.0
30	367.0
31	437.5
32	508.0
33	508.0
34	638.5
35	769.0
36	879.0
37	989.0
38	1309.0
39	1629.0
40	1629.0
41	1942.0
42	2255.0
43	2513.0
44	2771.0
45	3710.5
46	4650.0
47	4650.0
48	5151.5
49	5653.0
50	6502.0
51	7351.0
52	7611.5
53	7872.0
54	7872.0
55	7031.5
56	6191.0
57	5787.5
58	5384.0
59	5028.5
60	4673.0
61	4673.0
62	4164.0
63	3655.0
64	3049.0
65	2443.0
66	2090.0
67	1737.0
68	1737.0
69	1506.0
70	1275.0
71	1099.5
72	924.0
73	736.0
74	548.0
75	548.0
76	453.0
77	358.0
78	306.0
79	254.0
80	200.5
81	147.0
82	147.0
83	123.0
84	99.0
85	75.5
86	52.0
87	44.0
88	36.0
89	36.0
90	27.0
91	18.0
92	11.0
93	4.0
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	63349.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.29542692070909
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.10231118847307	56.87382594831804
2	5.758630693356541	7.520244992028288
3	2.250749443960932	4.40890937504933
4	1.1942752151629437	3.119228401395444
5	0.8364761628469201	2.730903408104311
6	0.5898849240885794	2.311007277147232
7	0.42065564258775745	1.9226822838560989
8	0.2852722173870999	1.4901576978326414
9	0.21032782129387873	1.2360100396217777
>10	1.281307417077652	14.773713870779334
>50	0.05802146794313896	2.5414765821086363
>100	0.012087805821487285	1.0718401237588597
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	166	0.26204044262735005	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	146	0.2304693049614043	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	129	0.20363383794535037	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	125	0.1973196104121612	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	113	0.17837692781259373	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	100	0.15785568832972896	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	100	0.15785568832972896	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	94	0.1483843470299452	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	86	0.1357558919635669	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	82	0.12944166443037775	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	75	0.11839176624729672	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	72	0.11365609559740486	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	68	0.10734186806421571	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	67	0.10576331118091839	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	67	0.10576331118091839	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	64	0.10102764053102654	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.003157113766594579	0.0
22	0.0	0.0	0.0	0.004735670649891869	0.0
23	0.0	0.0	0.0	0.007892784416486448	0.0
24	0.0	0.0	0.0	0.007892784416486448	0.0
25	0.0	0.0	0.0	0.009471341299783737	0.0
26	0.0	0.0	0.0	0.009471341299783737	0.0
27	0.0	0.0	0.0	0.022099796366162053	0.0
28	0.0	0.0	0.0	0.09313485611454009	0.0
29	0.0	0.0	0.0	0.21626229301172867	0.0
30	0.0	0.0	0.0	0.35359674185859286	0.0
31	0.0	0.0	0.0	0.8129567948981041	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGACC	20	0.0018288293	37.0	37
TGGGTAA	25	0.0054576807	29.6	10
CAAGCCT	25	0.0054576807	29.6	18
ACTCAGA	25	0.0054576807	29.6	18
GGTATCA	100	0.0	27.750002	1
CGTGCCA	55	6.106129E-7	26.90909	10
TAATACG	45	1.3040191E-4	24.666666	27
GCGGTAA	60	1.3043737E-6	24.666666	23
GTAATAC	60	1.3043737E-6	24.666666	26
CTAACTC	60	1.3043737E-6	24.666666	3
AACTCCG	60	1.3043737E-6	24.666666	5
CGGTAAT	60	1.3043737E-6	24.666666	24
GCTAACT	60	1.3043737E-6	24.666666	2
TAACTCC	60	1.3043737E-6	24.666666	4
GGCTAAC	60	1.3043737E-6	24.666666	1
GTGCCAG	70	1.8639366E-7	23.785715	11
CTTCCTC	40	0.001911208	23.125002	18
AACTGTC	40	0.001911208	23.125002	36
GGTAATA	65	2.6169891E-6	22.76923	25
TACGGAG	50	2.6632243E-4	22.2	30
>>END_MODULE