FastQCFastQC Report
Fri 10 Feb 2017
ERR1631767.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631767.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19683
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC520.2641873698115125No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA500.2540263171264543No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA480.24386526444139614No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA390.19814052735863436No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA350.17781842198851802No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA330.16765736930345984No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA320.16257684296093075No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT320.16257684296093075No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG310.15749631661840166No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC300.15241579027587257No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT300.15241579027587257No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT280.14225473759081442No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT260.13209368490575624No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT250.12701315856322715No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA240.12193263222069807No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT240.12193263222069807No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC230.11685210587816898No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT230.11685210587816898No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC220.11177157953563988No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG220.11177157953563988No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT210.1066910531931108No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG210.1066910531931108No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA210.1066910531931108No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT210.1066910531931108No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGTGGG200.10161052685058172No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC200.10161052685058172No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGTCT551.7823802E-523.54545437
TCTTATA400.001864826223.12537
CTCTTAT500.00679766118.536
GGCTGTC608.8041613E-418.536
TCTCTTA800.006012973413.87537
Produced by FastQC (version 0.11.5)