##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631767.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19683
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50734136056496	34.0	31.0	34.0	31.0	34.0
2	32.67957120357669	34.0	31.0	34.0	31.0	34.0
3	32.77376416196718	34.0	31.0	34.0	31.0	34.0
4	36.22674389066707	37.0	37.0	37.0	35.0	37.0
5	36.12874053751969	37.0	35.0	37.0	35.0	37.0
6	36.17863130620332	37.0	36.0	37.0	35.0	37.0
7	36.162119595590106	37.0	36.0	37.0	35.0	37.0
8	36.154143169232334	37.0	36.0	37.0	35.0	37.0
9	38.010719910582736	39.0	38.0	39.0	35.0	39.0
10	37.898948331047094	39.0	38.0	39.0	35.0	39.0
11	37.964893562973124	39.0	38.0	39.0	35.0	39.0
12	37.908652136361326	39.0	38.0	39.0	35.0	39.0
13	37.955697810293145	39.0	38.0	39.0	35.0	39.0
14	39.334247828074986	40.0	39.0	41.0	36.0	41.0
15	39.35655133871869	40.0	39.0	41.0	36.0	41.0
16	39.31143626479703	40.0	39.0	41.0	36.0	41.0
17	39.266016359294824	40.0	39.0	41.0	36.0	41.0
18	39.25428034344358	40.0	39.0	41.0	36.0	41.0
19	39.26276482243561	40.0	39.0	41.0	36.0	41.0
20	39.24640552761266	40.0	39.0	41.0	36.0	41.0
21	39.21886907483615	40.0	39.0	41.0	36.0	41.0
22	39.175938627241784	40.0	39.0	41.0	36.0	41.0
23	39.1275720164609	40.0	39.0	41.0	36.0	41.0
24	39.12868973225626	40.0	39.0	41.0	36.0	41.0
25	39.07681755829904	40.0	39.0	41.0	35.0	41.0
26	38.97104099984758	40.0	38.0	41.0	35.0	41.0
27	38.885129299395416	40.0	38.0	41.0	35.0	41.0
28	38.88162373621907	40.0	38.0	41.0	35.0	41.0
29	38.83117410963776	40.0	38.0	41.0	35.0	41.0
30	38.816897830615254	40.0	38.0	41.0	35.0	41.0
31	38.746938982878625	40.0	38.0	41.0	35.0	41.0
32	38.6765736930346	40.0	38.0	41.0	35.0	41.0
33	38.605192297922066	40.0	38.0	41.0	35.0	41.0
34	38.575725245135395	40.0	38.0	41.0	34.0	41.0
35	38.487070060458265	40.0	38.0	41.0	34.0	41.0
36	38.48727328151197	40.0	38.0	41.0	34.0	41.0
37	38.41838134430727	40.0	38.0	41.0	34.0	41.0
38	38.40700096530001	40.0	38.0	41.0	34.0	41.0
39	38.29172382258802	40.0	38.0	41.0	34.0	41.0
40	38.01493674744704	40.0	37.0	41.0	33.0	41.0
41	38.094802621551594	40.0	37.0	41.0	34.0	41.0
42	38.0699080424732	40.0	37.0	41.0	34.0	41.0
43	37.58253315043439	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	1.0
22	2.0
23	7.0
24	13.0
25	19.0
26	31.0
27	46.0
28	70.0
29	123.0
30	135.0
31	207.0
32	261.0
33	394.0
34	528.0
35	923.0
36	1283.0
37	2090.0
38	4783.0
39	8764.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.32672864908805	16.039221663364327	11.94939795762841	28.68465172991922
2	21.429660112787687	18.157801148198953	31.732967535436675	28.679571203576693
3	21.653203271858963	18.985926942031195	28.486511202560582	30.874358583549256
4	15.937611136513743	14.753848498704466	33.42986333384139	35.8786770309404
5	17.319514301681654	32.31722806482751	33.31809175430575	17.045165879185085
6	35.66529492455419	33.114870700604584	14.647157445511356	16.57267692932988
7	31.06741858456536	26.891225931006453	19.529543260681805	22.51181222374638
8	28.68465172991922	30.066554895087133	19.34156378600823	21.90722958898542
9	28.202001727378956	12.162780064014632	16.91815272062186	42.717065487984556
10	19.534623787024337	22.96397906823147	29.61946857694457	27.881928567799623
11	39.77036020931769	18.85891378346797	17.5024132500127	23.868312757201647
12	24.11725854798557	23.268810648783216	25.656658029771883	26.95727277345933
13	35.121678605903575	16.989280089417264	19.925824315399076	27.963216989280088
14	24.386526444139616	19.062134837169133	22.57277853985673	33.97856017883453
15	29.934461210181375	23.02494538434182	19.54986536605192	27.490728039424884
16	27.150332774475434	23.080831174109637	21.06386221612559	28.704973835289337
17	27.353553828176597	23.827668546461414	20.230655895950818	28.588121729411164
18	27.14017172179038	21.079103795153177	23.136716963877458	28.644007519178988
19	28.760859625057154	21.698928008941724	21.886907483615303	27.653304882385815
20	29.13681857440431	20.093481684702535	22.45592643397856	28.313773306914598
21	29.131738048061777	21.038459584412944	21.67860590357161	28.151196463953664
22	29.741401209165268	22.22730274856475	20.489762739419803	27.541533302850173
23	28.628765940151403	22.74043590916019	20.657420108723265	27.973378041965148
24	29.63471015597216	21.134989584920998	21.29756642788193	27.932733831224915
25	28.486511202560582	21.368693796677334	21.978356957780825	28.16643804298125
26	28.644007519178988	22.22730274856475	22.05964537926129	27.06904435299497
27	28.704973835289337	21.22135853274399	22.28318853833257	27.7904790936341
28	27.1096885637352	21.66844485088655	21.89706853630036	29.324798049077884
29	28.928516994360614	21.886907483615303	22.024081694863586	27.160493827160494
30	27.500889092109944	21.06894274246812	23.487273281511964	27.942894883909975
31	27.754915409236396	21.759894325052077	22.206980643194633	28.278209622516894
32	26.245999085505257	21.322969059594573	22.872529594065945	29.558502260834224
33	27.206218564243258	22.01392064217853	23.207844332672863	27.572016460905353
34	27.333231722806484	21.942793273383124	22.755677488187775	27.96829751562262
35	27.074124879337496	23.47203170248438	22.770919067215363	26.68292435096276
36	27.089366458365088	21.96819590509577	23.253569069755628	27.68886856678352
37	26.535589087029415	21.45506274450033	23.766702230351065	28.24264593811919
38	27.211299090585783	20.825077478026724	24.604989076868364	27.35863435451913
39	25.250215922369556	20.362749580856576	26.01229487374892	28.374739623024947
40	25.849718030787987	20.84539958339684	26.784534877813343	26.52034750800183
41	23.822588020118886	20.261139054005994	27.28750698572372	28.628765940151403
42	22.044403800233702	20.99781537367271	28.196921201036428	28.760859625057154
43	22.110450642686583	20.62185642432556	28.542396992328406	28.72529594065945
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.5
7	3.0
8	2.5
9	2.0
10	2.0
11	2.0
12	2.0
13	6.0
14	10.0
15	16.5
16	23.0
17	27.0
18	31.0
19	31.0
20	30.5
21	30.0
22	22.5
23	15.0
24	19.0
25	23.0
26	23.0
27	29.5
28	36.0
29	51.0
30	66.0
31	90.5
32	115.0
33	115.0
34	151.5
35	188.0
36	232.0
37	276.0
38	352.0
39	428.0
40	428.0
41	509.0
42	590.0
43	713.5
44	837.0
45	1068.5
46	1300.0
47	1300.0
48	1382.5
49	1465.0
50	1752.0
51	2039.0
52	2076.0
53	2113.0
54	2113.0
55	2055.0
56	1997.0
57	1882.5
58	1768.0
59	1646.5
60	1525.0
61	1525.0
62	1474.5
63	1424.0
64	1141.5
65	859.0
66	772.5
67	686.0
68	686.0
69	561.0
70	436.0
71	429.5
72	423.0
73	438.0
74	453.0
75	453.0
76	392.5
77	332.0
78	220.5
79	109.0
80	75.5
81	42.0
82	42.0
83	29.5
84	17.0
85	15.5
86	14.0
87	8.5
88	3.0
89	3.0
90	3.0
91	3.0
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	19683.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.66925773510135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.56843870779552	59.61489610323629
2	7.190257419966454	10.018797947467357
3	2.632538467147962	5.502210028959
4	1.2178225041931015	3.393791596809429
5	0.9261284912127179	3.2261342275059697
6	0.5979727266097863	2.49961896052431
7	0.40837161817253703	1.9915663262714016
8	0.29898636330489314	1.66641264034954
9	0.22606286005979725	1.4174668495656149
>10	0.9261284912127179	10.404917949499568
>50	0.00729235032450959	0.2641873698115125
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	52	0.2641873698115125	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	50	0.2540263171264543	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	48	0.24386526444139614	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	39	0.19814052735863436	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	35	0.17781842198851802	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	33	0.16765736930345984	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	32	0.16257684296093075	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	32	0.16257684296093075	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	31	0.15749631661840166	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	30	0.15241579027587257	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	30	0.15241579027587257	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	28	0.14225473759081442	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	26	0.13209368490575624	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	25	0.12701315856322715	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	24	0.12193263222069807	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	24	0.12193263222069807	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	23	0.11685210587816898	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	23	0.11685210587816898	No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC	22	0.11177157953563988	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	22	0.11177157953563988	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	21	0.1066910531931108	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	21	0.1066910531931108	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	21	0.1066910531931108	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	21	0.1066910531931108	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGTGGG	20	0.10161052685058172	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	20	0.10161052685058172	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.005080526342529086	0.0
22	0.0	0.0	0.0	0.005080526342529086	0.0
23	0.0	0.0	0.0	0.005080526342529086	0.0
24	0.0	0.0	0.0	0.005080526342529086	0.0
25	0.0	0.0	0.0	0.005080526342529086	0.0
26	0.0	0.0	0.0	0.010161052685058172	0.0
27	0.0	0.0	0.0	0.035563684397703604	0.0
28	0.0	0.0	0.0	0.15749631661840166	0.0
29	0.0	0.0	0.0	0.28450947518162883	0.0
30	0.0	0.0	0.0	0.4674084235126759	0.0
31	0.0	0.0	0.0	0.8687700045724737	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTGTCT	55	1.7823802E-5	23.545454	37
TCTTATA	40	0.0018648262	23.125	37
CTCTTAT	50	0.006797661	18.5	36
GGCTGTC	60	8.8041613E-4	18.5	36
TCTCTTA	80	0.0060129734	13.875	37
>>END_MODULE