FastQCFastQC Report
Fri 10 Feb 2017
ERR1631766.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631766.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285803
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA7230.252971452364041No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6990.24457405975444624No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6300.2204315560018614No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5960.20853524980493557No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5840.20433655350013824No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5680.19873829176040841No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG5460.1910406818682799No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5190.18159361518248585No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5040.17634524480148914No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4930.1724964398554249No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4800.16794785219189443No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4340.15185284969017118No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3940.13785719534084667No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA3380.11826327925179231No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3360.11756349653432609No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3290.1151142570231943No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3140.1098658866421976No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2970.10391773354373468No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT2940.10286805946753534No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2890.10111860267386977No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG303.5927744E-430.8333326
TTGCGCG250.005489521729.68
GGTATCA4800.027.3645821
ATACGGC551.8961813E-523.54545429
GGGGACT400.001927657523.12500231
CCGCTTA400.001927657523.12500225
GCGGTAA2500.022.93999923
ATAACGC652.6711514E-622.7692335
TATACAC903.79805E-922.6111137
CACTGTG753.7204518E-722.27
TTTTACG502.6952245E-422.24
TAATACG2500.022.19999927
CGCGGTA2650.021.6415122
TACGGCT603.7136917E-521.58333230
GGTAATA2750.020.85454425
CGGTAAT2750.020.85454424
GACCCAT450.00381888420.55555518
TCAATGC450.00381888420.55555532
TCGGAGG450.00381888420.55555518
GTACTGC450.00381888420.55555511
Produced by FastQC (version 0.11.5)