##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631766.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	285803
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61594874791377	34.0	31.0	34.0	31.0	34.0
2	32.783900099019256	34.0	31.0	34.0	31.0	34.0
3	32.87103704299815	34.0	31.0	34.0	31.0	34.0
4	36.29920959542062	37.0	37.0	37.0	35.0	37.0
5	36.20600553528129	37.0	35.0	37.0	35.0	37.0
6	36.26486775856097	37.0	37.0	37.0	35.0	37.0
7	36.25853822388149	37.0	37.0	37.0	35.0	37.0
8	36.24188339520579	37.0	37.0	37.0	35.0	37.0
9	38.08043652445915	39.0	38.0	39.0	37.0	39.0
10	38.00541631823319	39.0	38.0	39.0	35.0	39.0
11	38.08341060100839	39.0	38.0	39.0	37.0	39.0
12	38.01107056259031	39.0	38.0	39.0	35.0	39.0
13	38.07823920672631	39.0	38.0	39.0	37.0	39.0
14	39.460019663894364	40.0	39.0	41.0	37.0	41.0
15	39.47223087231415	41.0	39.0	41.0	37.0	41.0
16	39.40768991228224	40.0	39.0	41.0	37.0	41.0
17	39.380093980818955	40.0	39.0	41.0	37.0	41.0
18	39.406615745810925	40.0	39.0	41.0	37.0	41.0
19	39.42790663498984	41.0	39.0	41.0	37.0	41.0
20	39.431528010552725	41.0	39.0	41.0	37.0	41.0
21	39.386234574164725	40.0	39.0	41.0	37.0	41.0
22	39.35528668348478	40.0	39.0	41.0	36.0	41.0
23	39.292138291060624	40.0	39.0	41.0	36.0	41.0
24	39.3025580557237	40.0	39.0	41.0	36.0	41.0
25	39.23975605574469	40.0	39.0	41.0	36.0	41.0
26	39.186894469267294	40.0	39.0	41.0	36.0	41.0
27	39.07513916928794	40.0	39.0	41.0	35.0	41.0
28	39.076377784697854	40.0	39.0	41.0	35.0	41.0
29	39.053522881145405	40.0	39.0	41.0	35.0	41.0
30	39.021612789228946	40.0	39.0	41.0	35.0	41.0
31	38.92803784424936	40.0	38.0	41.0	35.0	41.0
32	38.894511254255555	40.0	38.0	41.0	35.0	41.0
33	38.83063158889165	40.0	38.0	41.0	35.0	41.0
34	38.806629041682555	40.0	38.0	41.0	35.0	41.0
35	38.73070961466465	40.0	38.0	41.0	35.0	41.0
36	38.701392217716396	40.0	38.0	41.0	35.0	41.0
37	38.65163066867738	40.0	38.0	41.0	35.0	41.0
38	38.61649807734698	40.0	38.0	41.0	35.0	41.0
39	38.555665965717644	40.0	38.0	41.0	35.0	41.0
40	38.22166667249819	40.0	38.0	41.0	34.0	41.0
41	38.307855410894916	40.0	38.0	41.0	34.0	41.0
42	38.29754061363946	40.0	37.0	41.0	34.0	41.0
43	37.83738099320161	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	1.0
18	5.0
19	5.0
20	9.0
21	25.0
22	39.0
23	72.0
24	125.0
25	235.0
26	366.0
27	558.0
28	900.0
29	1258.0
30	1857.0
31	2461.0
32	3470.0
33	4789.0
34	7054.0
35	10856.0
36	16604.0
37	29405.0
38	68546.0
39	137160.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.58667683684216	16.396258961592423	12.961725384268185	28.055338817297233
2	20.768851271680145	19.488948681434415	32.55599136468126	27.186208682204178
3	21.071157405625556	19.68943642998849	29.469249797937742	29.77015636644822
4	15.660087542817955	16.062112714002303	34.204679447031694	34.07312029614805
5	16.839921204466012	32.61827202653576	33.65709947061438	16.88470729838385
6	33.53603706049272	33.754369268342174	15.849028876533836	16.860564794631266
7	29.878272796296752	27.676406475789268	20.394467517835714	22.05085321007827
8	27.320566963957692	30.40870809613615	20.59985374541205	21.670871194494108
9	28.36639223521097	12.658369576246574	18.024653345136336	40.950584843406126
10	19.14920417210456	23.380440373264104	29.815992134442254	27.65436332018908
11	38.78335776741322	19.53548423214592	18.752427371301213	22.92873062913965
12	22.739089512706304	24.088620483339923	26.6211341378502	26.551155866103578
13	34.93735195221884	17.796174287883613	20.87416857065881	26.39230518923874
14	24.133406577257762	20.367875774571996	23.332505257117663	32.16621239105258
15	29.86602659874109	24.052931564749144	20.081314751769575	25.999727084740186
16	25.335283394506007	24.361185851793017	22.592485033397132	27.711045720303844
17	26.856960913636318	23.735230211019477	21.773739253961644	27.63406962138256
18	26.49797237957614	21.568353026385306	23.49940343523336	28.434271158805192
19	27.99130869864907	22.47317208006914	23.404232985657952	26.13128623562384
20	29.93355563097658	20.79999160260739	23.033348145400854	26.233104621015173
21	27.361154361570733	22.135526918891685	22.164567901666533	28.338750817871052
22	28.300262768410413	23.158959143186042	21.392357672942552	27.148420415461
23	27.865697700863883	22.2149522573241	22.546649265403094	27.372700776408927
24	27.72504137465317	21.67611956487511	23.139015335738254	27.459823724733468
25	27.79327018960613	21.988222656865045	23.26007774585991	26.95842940766892
26	27.474869053158997	22.9070373648982	22.510960346812315	27.107133235130494
27	26.793980469064355	21.861561985003654	24.30975182205925	27.034705723872737
28	25.965787622943076	23.002557705832338	22.33846390695689	28.693190764267694
29	27.215249664979023	23.54453942050993	22.43853283555456	26.80167807895648
30	26.67746664660623	22.172615402917394	24.035087105453755	27.11483084502262
31	27.745684964818423	22.508860998659916	22.97456639713369	26.77088763938797
32	25.384268184728644	22.45847664300235	23.24328296064072	28.91397221162829
33	25.715615301448903	22.003267985290567	24.87062767010843	27.410489043152104
34	26.037865242842095	21.764991969993318	24.384278681469404	27.812864105695184
35	25.38146905385878	23.678197919545983	24.99169008023009	25.94864294636515
36	25.962288709355747	22.23944465243542	25.200925112752493	26.597341525456347
37	26.406300843588067	22.012365160617627	24.785953961295018	26.79538003449929
38	25.542768970234743	21.268146240592294	26.091398620728263	27.097686168444696
39	24.900018544242013	20.320990332501758	27.11168182279402	27.667309300462207
40	24.473850869305082	21.405303653215675	27.77752507846314	26.343320399016108
41	22.889892688320277	20.33288663869868	28.97730254755898	27.799918125422057
42	21.435394310066723	21.107196215575065	29.650493521761494	27.806915952596718
43	20.654786688733147	21.71915620199928	29.592061664853063	28.03399544441451
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	2.0
2	2.0
3	8.5
4	15.0
5	15.0
6	20.0
7	25.0
8	27.5
9	30.0
10	41.0
11	52.0
12	52.0
13	127.0
14	202.0
15	320.5
16	439.0
17	453.5
18	468.0
19	468.0
20	551.0
21	634.0
22	554.5
23	475.0
24	566.5
25	658.0
26	658.0
27	758.0
28	858.0
29	1227.5
30	1597.0
31	1950.0
32	2303.0
33	2303.0
34	2892.5
35	3482.0
36	4037.0
37	4592.0
38	5718.0
39	6844.0
40	6844.0
41	8030.0
42	9216.0
43	10706.5
44	12197.0
45	16858.5
46	21520.0
47	21520.0
48	24112.0
49	26704.0
50	30285.0
51	33866.0
52	34220.5
53	34575.0
54	34575.0
55	31330.5
56	28086.0
57	26556.5
58	25027.0
59	22774.5
60	20522.0
61	20522.0
62	18785.5
63	17049.0
64	13976.5
65	10904.0
66	9405.0
67	7906.0
68	7906.0
69	6725.0
70	5544.0
71	4765.0
72	3986.0
73	3189.5
74	2393.0
75	2393.0
76	1902.0
77	1411.0
78	1186.0
79	961.0
80	736.0
81	511.0
82	511.0
83	427.0
84	343.0
85	265.5
86	188.0
87	152.0
88	116.0
89	116.0
90	93.5
91	71.0
92	46.5
93	22.0
94	14.0
95	6.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	285803.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.30182117364078
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.88702302511298	47.18123586619695
2	5.980258213161669	6.49477824126603
3	2.0144362803873443	3.28162675989863
4	1.1179011945181536	2.428162830180968
5	0.7155204019564898	1.9427030456566443
6	0.48189830096217356	1.5700773217637565
7	0.3769951938083928	1.4330067918253777
8	0.28457106399249027	1.2362181622490316
9	0.2143133399996276	1.047384419740691
>10	1.6729306018908403	17.40262236508735
>50	0.16535990567579723	6.017558061561888
>100	0.0830016647166453	8.088746831749361
>500	0.005790813817440369	1.8758793028233047
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	723	0.252971452364041	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	699	0.24457405975444624	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	630	0.2204315560018614	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	596	0.20853524980493557	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	584	0.20433655350013824	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	568	0.19873829176040841	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	546	0.1910406818682799	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	519	0.18159361518248585	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	504	0.17634524480148914	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	493	0.1724964398554249	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	480	0.16794785219189443	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	434	0.15185284969017118	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	394	0.13785719534084667	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	338	0.11826327925179231	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	336	0.11756349653432609	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	329	0.1151142570231943	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	314	0.1098658866421976	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	297	0.10391773354373468	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	294	0.10286805946753534	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	289	0.10111860267386977	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.498913587331134E-4	0.0
8	0.0	0.0	0.0	3.498913587331134E-4	0.0
9	0.0	0.0	0.0	3.498913587331134E-4	0.0
10	0.0	0.0	0.0	3.498913587331134E-4	0.0
11	0.0	0.0	0.0	3.498913587331134E-4	0.0
12	0.0	0.0	0.0	3.498913587331134E-4	0.0
13	0.0	0.0	0.0	3.498913587331134E-4	0.0
14	0.0	0.0	0.0	3.498913587331134E-4	0.0
15	3.498913587331134E-4	0.0	0.0	3.498913587331134E-4	0.0
16	3.498913587331134E-4	0.0	0.0	3.498913587331134E-4	0.0
17	6.997827174662268E-4	0.0	0.0	0.00104967407619934	0.0
18	6.997827174662268E-4	0.0	0.0	0.0017494567936655668	0.0
19	6.997827174662268E-4	0.0	0.0	0.0024492395111317938	0.0
20	6.997827174662268E-4	0.0	0.0	0.0034989135873311336	0.0
21	6.997827174662268E-4	0.0	0.0	0.0048984790222635875	0.0
22	6.997827174662268E-4	0.0	0.0	0.009447066685794061	0.0
23	6.997827174662268E-4	0.0	0.0	0.012596088914392081	0.0
24	6.997827174662268E-4	0.0	0.0	0.016095002501723216	0.0
25	6.997827174662268E-4	0.0	0.0	0.018194350654121894	3.498913587331134E-4
26	6.997827174662268E-4	0.0	0.0	0.020993481523986803	3.498913587331134E-4
27	6.997827174662268E-4	0.0	0.0	0.050384355657568325	3.498913587331134E-4
28	6.997827174662268E-4	0.0	0.0	0.1515029583314381	3.498913587331134E-4
29	6.997827174662268E-4	0.0	0.0	0.3292477685678597	3.498913587331134E-4
30	6.997827174662268E-4	0.0	0.0	0.5395324751664609	3.498913587331134E-4
31	6.997827174662268E-4	0.0	0.0	1.1336480022952873	3.498913587331134E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGTAG	30	3.5927744E-4	30.833332	6
TTGCGCG	25	0.0054895217	29.6	8
GGTATCA	480	0.0	27.364582	1
ATACGGC	55	1.8961813E-5	23.545454	29
GGGGACT	40	0.0019276575	23.125002	31
CCGCTTA	40	0.0019276575	23.125002	25
GCGGTAA	250	0.0	22.939999	23
ATAACGC	65	2.6711514E-6	22.76923	35
TATACAC	90	3.79805E-9	22.61111	37
CACTGTG	75	3.7204518E-7	22.2	7
TTTTACG	50	2.6952245E-4	22.2	4
TAATACG	250	0.0	22.199999	27
CGCGGTA	265	0.0	21.64151	22
TACGGCT	60	3.7136917E-5	21.583332	30
GGTAATA	275	0.0	20.854544	25
CGGTAAT	275	0.0	20.854544	24
GACCCAT	45	0.003818884	20.555555	18
TCAATGC	45	0.003818884	20.555555	32
TCGGAGG	45	0.003818884	20.555555	18
GTACTGC	45	0.003818884	20.555555	11
>>END_MODULE