FastQCFastQC Report
Fri 10 Feb 2017
ERR1631765.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631765.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866069
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25690.29662763590429864No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25140.2902771026327001No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19760.22815734081233713No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA18940.21868927302559032No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC11250.1298972714645138No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG10820.12493230908853684No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA10430.12042920367776701No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG10290.11881270429954195No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10240.11823538309303301No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC10080.11638795523220435No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA9570.11049927892581309No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9210.10634256623894864No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC8880.10253224627598957No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8770.10126213962166986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9550.029.6387421
TTACGAG659.386349E-825.6153856
ATTGGAC502.7013576E-422.1999993
CTTACGA855.1770257E-821.76470619
TTTTACG851.2445835E-619.5882344
GTATCAA14550.019.326462
CAACCGT609.234311E-418.515
TATTAGG500.00703351718.4999982
TATACAG500.00703351718.4999985
CCTTACG1054.793892E-717.61904718
GCCCTTA1054.793892E-717.61904716
CTTATAC9800.017.55612437
TACGAGG953.6043111E-617.5263167
ATATGAT752.0665253E-417.2666663
TATTTCG650.001579509117.07692329
CCAACCG1107.8021367E-716.81818214
TCTTATA17250.016.19420237
GACCGCT700.00259180215.85714219
CGTCCAA700.00259180215.85714210
CTAGTTC855.3622556E-415.2352933
Produced by FastQC (version 0.11.5)