##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631765.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	866069
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64942862520192	34.0	31.0	34.0	31.0	34.0
2	32.816887569004315	34.0	31.0	34.0	31.0	34.0
3	32.909321312736054	34.0	31.0	34.0	31.0	34.0
4	36.30965084768073	37.0	37.0	37.0	35.0	37.0
5	36.23246993022496	37.0	37.0	37.0	35.0	37.0
6	36.28819874628927	37.0	37.0	37.0	35.0	37.0
7	36.27320917848347	37.0	37.0	37.0	35.0	37.0
8	36.25887660221068	37.0	37.0	37.0	35.0	37.0
9	38.0796229861593	39.0	38.0	39.0	37.0	39.0
10	38.02412278929277	39.0	38.0	39.0	35.0	39.0
11	38.09010944855433	39.0	38.0	39.0	37.0	39.0
12	38.04060992830825	39.0	38.0	39.0	35.0	39.0
13	38.08838787671652	39.0	38.0	39.0	37.0	39.0
14	39.5020847068767	41.0	39.0	41.0	37.0	41.0
15	39.49294686682008	41.0	39.0	41.0	37.0	41.0
16	39.460205826556546	41.0	39.0	41.0	37.0	41.0
17	39.436113057966516	41.0	39.0	41.0	37.0	41.0
18	39.45365323086267	41.0	39.0	41.0	37.0	41.0
19	39.47257435608479	41.0	39.0	41.0	37.0	41.0
20	39.45537133877324	41.0	39.0	41.0	37.0	41.0
21	39.4267188872942	41.0	39.0	41.0	37.0	41.0
22	39.39970602804165	41.0	39.0	41.0	36.0	41.0
23	39.32963886249248	40.0	39.0	41.0	36.0	41.0
24	39.335219249274594	41.0	39.0	41.0	36.0	41.0
25	39.27483837892824	40.0	39.0	41.0	36.0	41.0
26	39.209273164147426	40.0	39.0	41.0	36.0	41.0
27	39.11386968012941	40.0	39.0	41.0	35.0	41.0
28	39.102238967103084	40.0	39.0	41.0	35.0	41.0
29	39.069815453503125	40.0	39.0	41.0	35.0	41.0
30	39.004872590982934	40.0	39.0	41.0	35.0	41.0
31	38.91323439587377	40.0	38.0	41.0	35.0	41.0
32	38.85228659610262	40.0	38.0	41.0	35.0	41.0
33	38.79298416177002	40.0	38.0	41.0	35.0	41.0
34	38.73841460668838	40.0	38.0	41.0	35.0	41.0
35	38.6709003555144	40.0	38.0	41.0	35.0	41.0
36	38.60517695472301	40.0	38.0	41.0	35.0	41.0
37	38.550386862940485	40.0	38.0	41.0	35.0	41.0
38	38.50559943838193	40.0	38.0	41.0	35.0	41.0
39	38.437265391094705	40.0	38.0	41.0	35.0	41.0
40	38.097449510373885	40.0	37.0	41.0	34.0	41.0
41	38.16004844879565	40.0	37.0	41.0	34.0	41.0
42	38.123301954001356	40.0	37.0	41.0	34.0	41.0
43	37.65924424035499	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	1.0
16	1.0
17	1.0
18	5.0
19	14.0
20	23.0
21	67.0
22	141.0
23	203.0
24	482.0
25	695.0
26	1185.0
27	1936.0
28	2982.0
29	4344.0
30	6024.0
31	8162.0
32	11099.0
33	14649.0
34	21309.0
35	32186.0
36	49256.0
37	91315.0
38	195407.0
39	424581.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.357746322752575	17.15105840296789	12.774039943699636	26.717155330579896
2	20.411191256123935	19.561028047418855	33.74280802106991	26.284972675387298
3	21.241379151083805	20.434168640142993	28.798744672768567	29.525707536004635
4	15.723804916236467	15.886263103748085	32.94391093550283	35.44602104451262
5	17.695010443740625	32.921972729655494	32.10610240061704	17.276914425986845
6	34.86742973134935	33.01596062207515	15.524513635749576	16.592096010825927
7	30.588324948705008	28.133555178628956	20.0865058095833	21.191614063082735
8	27.167696800139478	31.748856038029306	19.748657439534263	21.334789722296954
9	27.240208343677004	14.211107890941715	18.783145453768697	39.76553831161259
10	19.577885826648917	24.21977925546348	30.635087966432234	25.56724695145537
11	37.66097158540486	20.67687447535935	20.015957158147906	21.64619678108788
12	22.76596899323264	23.494548355846938	28.039913678933203	25.69956897198722
13	33.32679035965956	18.076966153966946	22.317967736981696	26.278275749391796
14	23.783901744549222	20.285335233104984	24.46698819609061	31.463774826255182
15	28.046495140687405	24.977340142644525	21.33017115264488	25.64599356402319
16	25.332161756164922	24.29783308258349	23.32250663630727	27.04749852494432
17	25.330314328304095	24.260884525366915	23.99208377161635	26.41671737471264
18	25.976221294146306	22.588731382834393	24.763962224718817	26.671085098300484
19	27.118278104862313	23.401599641598995	24.363070378918998	25.11705187461969
20	27.343202446918202	22.461027931954614	24.527260530050146	25.66850909107704
21	26.61035090737574	23.246069308565485	23.960792962223564	26.182786821835215
22	26.596033341454316	23.67721278558637	23.402869748253316	26.323884124705998
23	26.640487074355505	23.325393242339814	23.884702027205684	26.149417656098993
24	26.61947258243858	22.967569558545566	23.935852686102375	26.477105172913472
25	26.79994319159328	23.13164424543541	24.33593628221308	25.73247628075823
26	26.68193873698285	23.27227969134099	24.305222794026804	25.74055877764936
27	26.389929670730623	23.149310274354583	24.725974489330525	25.734785565584268
28	25.773350622179063	23.862879285599647	24.080875773177425	26.282894319043866
29	26.132790805351537	24.009057015087713	23.74060265406105	26.1175495254997
30	25.806950716397886	23.93654547155019	24.578411188946838	25.678092623105087
31	26.25552929385534	22.958216955000122	24.133758395693647	26.652495355450895
32	24.73844462739112	23.368692332827983	24.718353849404608	27.174509190376284
33	25.217967621517456	22.925771503194316	25.62544092907147	26.230819946216755
34	24.948243153836472	22.637803685387652	25.35987317407735	27.05407998669852
35	25.173052031651054	23.358993336558633	25.671626625592186	25.79632800619812
36	25.24948935939284	22.92057561233574	25.72000614269764	26.10992888557378
37	25.223394440858637	22.296722316582166	25.780624869381075	26.699258373178118
38	24.276356733701355	21.985084329308634	26.687481020565336	27.05107791642467
39	24.057667460675766	21.242533793496822	27.917637047394606	26.782161698432805
40	23.794408990507684	22.15770337005481	28.143831496104816	25.904056143332692
41	23.247916736426312	21.514105689038633	28.66018758320642	26.577789991328636
42	22.492203277106096	22.003443143675618	29.115232158176774	26.389121421041512
43	22.008177177568992	22.243955158307248	28.65718551293257	27.090682151191185
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	47.0
1	49.0
2	51.0
3	122.5
4	194.0
5	194.0
6	244.0
7	294.0
8	320.0
9	346.0
10	494.0
11	642.0
12	642.0
13	1296.0
14	1950.0
15	3393.5
16	4837.0
17	4746.0
18	4655.0
19	4655.0
20	5306.5
21	5958.0
22	5083.5
23	4209.0
24	4626.0
25	5043.0
26	5043.0
27	5843.5
28	6644.0
29	7981.0
30	9318.0
31	10941.5
32	12565.0
33	12565.0
34	15298.5
35	18032.0
36	19600.0
37	21168.0
38	24085.5
39	27003.0
40	27003.0
41	30617.0
42	34231.0
43	39363.0
44	44495.0
45	53834.0
46	63173.0
47	63173.0
48	69043.5
49	74914.0
50	77250.0
51	79586.0
52	79279.5
53	78973.0
54	78973.0
55	76286.0
56	73599.0
57	70364.5
58	67130.0
59	62351.0
60	57572.0
61	57572.0
62	52758.0
63	47944.0
64	42005.5
65	36067.0
66	31905.0
67	27743.0
68	27743.0
69	24226.5
70	20710.0
71	17414.5
72	14119.0
73	11545.0
74	8971.0
75	8971.0
76	7328.5
77	5686.0
78	4583.0
79	3480.0
80	2893.0
81	2306.0
82	2306.0
83	1766.0
84	1226.0
85	970.5
86	715.0
87	519.0
88	323.0
89	323.0
90	219.5
91	116.0
92	72.5
93	29.0
94	17.0
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	866069.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.95171616129147
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.48396085759515	55.07265472572863
2	7.17923292356522	9.038900665201528
3	2.045584676571975	3.8631919783033855
4	0.9226460270623509	2.3232860325188947
5	0.534466739509206	1.682279924161722
6	0.3603733492531889	1.3611672476568426
7	0.2325333206919591	1.0246860121569943
8	0.17298882045718478	0.8711954499597847
9	0.1358929125250087	0.7699222851845002
>10	0.7896931386066947	9.422333625183162
>50	0.07619230359746207	3.3058019177881537
>100	0.05999387083449347	7.531538553094504
>500	0.004600756950189596	1.9647038515081376
>1k	0.0018403027800758386	1.7683377315537596
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2569	0.29662763590429864	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2514	0.2902771026327001	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1976	0.22815734081233713	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1894	0.21868927302559032	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	1125	0.1298972714645138	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1082	0.12493230908853684	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	1043	0.12042920367776701	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	1029	0.11881270429954195	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1024	0.11823538309303301	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	1008	0.11638795523220435	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	957	0.11049927892581309	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	921	0.10634256623894864	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	888	0.10253224627598957	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	877	0.10126213962166986	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	1.1546424130179004E-4	0.0	0.0	0.0	0.0
9	1.1546424130179004E-4	0.0	0.0	0.0	0.0
10	1.1546424130179004E-4	0.0	0.0	0.0	0.0
11	1.1546424130179004E-4	0.0	0.0	0.0	0.0
12	1.1546424130179004E-4	0.0	0.0	0.0	0.0
13	1.1546424130179004E-4	0.0	0.0	0.0	0.0
14	1.1546424130179004E-4	0.0	0.0	0.0	0.0
15	1.1546424130179004E-4	0.0	0.0	1.1546424130179004E-4	0.0
16	1.1546424130179004E-4	0.0	0.0	4.6185696520716015E-4	0.0
17	1.1546424130179004E-4	0.0	0.0	5.773212065089502E-4	0.0
18	2.3092848260358007E-4	0.0	0.0	8.082496891125303E-4	0.0
19	2.3092848260358007E-4	0.0	0.0	0.0010391781717161103	0.0
20	2.3092848260358007E-4	0.0	0.0	0.0012701066543196905	0.0
21	3.463927239053701E-4	0.0	0.0	0.0015010351369232706	0.0
22	3.463927239053701E-4	0.0	0.0	0.0024247490673375907	0.0
23	4.6185696520716015E-4	0.0	0.0	0.003694855721657281	0.0
24	4.6185696520716015E-4	0.0	0.0	0.0060041405476930826	0.0
25	4.6185696520716015E-4	0.0	0.0	0.007158782960710983	0.0
26	4.6185696520716015E-4	0.0	0.0	0.011777352612782585	0.0
27	5.773212065089502E-4	0.0	0.0	0.042144448075153364	0.0
28	6.927854478107402E-4	0.0	0.0	0.1633819014420329	0.0
29	6.927854478107402E-4	0.0	0.0	0.3248009107819354	0.0
30	6.927854478107402E-4	0.0	0.0	0.5324056166425539	0.0
31	6.927854478107402E-4	0.0	0.0	1.1347825635139925	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	955	0.0	29.638742	1
TTACGAG	65	9.386349E-8	25.615385	6
ATTGGAC	50	2.7013576E-4	22.199999	3
CTTACGA	85	5.1770257E-8	21.764706	19
TTTTACG	85	1.2445835E-6	19.588234	4
GTATCAA	1455	0.0	19.32646	2
CAACCGT	60	9.234311E-4	18.5	15
TATTAGG	50	0.007033517	18.499998	2
TATACAG	50	0.007033517	18.499998	5
CCTTACG	105	4.793892E-7	17.619047	18
GCCCTTA	105	4.793892E-7	17.619047	16
CTTATAC	980	0.0	17.556124	37
TACGAGG	95	3.6043111E-6	17.526316	7
ATATGAT	75	2.0665253E-4	17.266666	3
TATTTCG	65	0.0015795091	17.076923	29
CCAACCG	110	7.8021367E-7	16.818182	14
TCTTATA	1725	0.0	16.194202	37
GACCGCT	70	0.002591802	15.857142	19
CGTCCAA	70	0.002591802	15.857142	10
CTAGTTC	85	5.3622556E-4	15.235293	3
>>END_MODULE