ERR1631764.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1631764.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28391
Sequences flagged as poor quality	0
Sequence length	43
%GC	54

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	78	0.274734951216935	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	70	0.24655700750237752	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	69	0.24303476453805783	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	67	0.23599027860941846	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	58	0.20429009193054137	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	57	0.20076784896622168	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	54	0.19020112007326265	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	52	0.1831566341446233	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	52	0.1831566341446233	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	51	0.1796343911803036	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	45	0.15850093339438556	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	42	0.14793420450142653	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	42	0.14793420450142653	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	38	0.1338452326441478	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	38	0.1338452326441478	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	37	0.1303229896798281	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	37	0.1303229896798281	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	33	0.1162340178225494	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	33	0.1162340178225494	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	32	0.11271177485822972	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	32	0.11271177485822972	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	32	0.11271177485822972	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	32	0.11271177485822972	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	31	0.10918953189391005	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	30	0.10566728892959036	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	30	0.10566728892959036	No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	29	0.10214504596527069	TruSeq Adapter, Index 3 (95% over 21bp)
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	29	0.10214504596527069	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATGAG	30	8.029485E-6	37.0	33
GAGCAAA	30	8.029485E-6	37.0	37
AATGAGC	30	8.029485E-6	37.0	34
TGAGCAA	35	2.3077757E-5	31.714285	36
CGGAGGG	30	3.512452E-4	30.833334	32
ATACGGA	30	3.512452E-4	30.833334	29
ACGGAGG	30	3.512452E-4	30.833334	31
GGAGGGT	25	0.005407511	29.6	33
GAGGGTG	25	0.005407511	29.6	34
GGGTGCA	25	0.005407511	29.6	36
GGTGCAA	25	0.005407511	29.6	37
GTATCAA	45	3.830137E-6	28.777777	2
GGTATCA	45	3.830137E-6	28.777777	1
ACGTCAA	40	5.741416E-5	27.75	29
TCAATGA	40	5.741416E-5	27.75	32
TCTGCGG	40	5.741416E-5	27.75	19
CTGCGGG	40	5.741416E-5	27.75	20
TGCGGGT	40	5.741416E-5	27.75	21
GTGCTTC	40	5.741416E-5	27.75	11
GGTAACG	40	5.741416E-5	27.75	25

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content