##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631764.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28391
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62104187946885	34.0	31.0	34.0	31.0	34.0
2	32.79320911556479	34.0	31.0	34.0	31.0	34.0
3	32.865626430911206	34.0	31.0	34.0	31.0	34.0
4	36.30002465570075	37.0	37.0	37.0	35.0	37.0
5	36.20598076855342	37.0	35.0	37.0	35.0	37.0
6	36.25060758691134	37.0	37.0	37.0	35.0	37.0
7	36.262618435419675	37.0	37.0	37.0	35.0	37.0
8	36.222394420767145	37.0	37.0	37.0	35.0	37.0
9	38.07488288542144	39.0	38.0	39.0	37.0	39.0
10	38.00405057940897	39.0	38.0	39.0	35.0	39.0
11	38.08108203303864	39.0	38.0	39.0	37.0	39.0
12	37.99788665422141	39.0	38.0	39.0	35.0	39.0
13	38.089535416153005	39.0	38.0	39.0	37.0	39.0
14	39.45007220598077	40.0	39.0	41.0	37.0	41.0
15	39.482969955267514	41.0	39.0	41.0	37.0	41.0
16	39.396921559649186	40.0	39.0	41.0	37.0	41.0
17	39.3880455073791	40.0	39.0	41.0	37.0	41.0
18	39.407910957697865	40.0	39.0	41.0	37.0	41.0
19	39.438378359339225	40.0	39.0	41.0	37.0	41.0
20	39.42379627346694	40.0	39.0	41.0	37.0	41.0
21	39.38994751857983	40.0	39.0	41.0	37.0	41.0
22	39.39413898770737	40.0	39.0	41.0	37.0	41.0
23	39.31298650980945	40.0	39.0	41.0	36.0	41.0
24	39.3197139938713	40.0	39.0	41.0	36.0	41.0
25	39.25071325420028	40.0	39.0	41.0	36.0	41.0
26	39.198161389172625	40.0	39.0	41.0	36.0	41.0
27	39.09013419745694	40.0	39.0	41.0	36.0	41.0
28	39.07847557324504	40.0	39.0	41.0	35.0	41.0
29	39.035504209080344	40.0	39.0	41.0	35.0	41.0
30	39.02211968581593	40.0	39.0	41.0	35.0	41.0
31	38.920960867880666	40.0	38.0	41.0	35.0	41.0
32	38.896234722271146	40.0	38.0	41.0	35.0	41.0
33	38.824204853650805	40.0	38.0	41.0	35.0	41.0
34	38.804515515480254	40.0	38.0	41.0	35.0	41.0
35	38.73146419640027	40.0	38.0	41.0	35.0	41.0
36	38.675108308971154	40.0	38.0	41.0	35.0	41.0
37	38.61353950195485	40.0	38.0	41.0	35.0	41.0
38	38.58905991335282	40.0	38.0	41.0	35.0	41.0
39	38.51421225036103	40.0	38.0	41.0	34.0	41.0
40	38.18410763974499	40.0	38.0	41.0	34.0	41.0
41	38.26240710084181	40.0	37.0	41.0	34.0	41.0
42	38.257863407417844	40.0	37.0	41.0	34.0	41.0
43	37.785178401606146	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	1.0
20	1.0
21	2.0
22	2.0
23	11.0
24	11.0
25	28.0
26	27.0
27	69.0
28	92.0
29	128.0
30	184.0
31	240.0
32	352.0
33	519.0
34	658.0
35	1074.0
36	1625.0
37	2915.0
38	6872.0
39	13579.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.74946285794794	16.54397520340953	12.588496354478531	28.118065584163997
2	20.886900778415697	19.502659293438064	31.763587052234865	27.84685287591138
3	20.886900778415697	19.59775985347469	29.699552675143533	29.81578669296608
4	15.779648480152161	16.135395019548447	33.93328871825578	34.1516677820436
5	16.73769856644711	33.39086330175056	33.78183227079004	16.089605861012295
6	34.6342150681554	33.55640872107358	15.286534465147405	16.522841745623612
7	30.61533584586665	27.353738860906624	19.805572188369553	22.225353104857174
8	27.498150822443733	30.978126871191574	20.61568807016308	20.908034236201615
9	27.987742594484168	12.366595047726392	17.931738931351486	41.71392342643795
10	19.81261667429819	23.16226973336621	29.667852488464654	27.357261103870943
11	38.737628121587825	19.393469761544154	19.07294565179106	22.795956465076962
12	22.93684618364975	23.57789440315593	26.684512697685886	26.800746715508435
13	35.53590926702124	17.023000246557007	20.70726638723539	26.73382409918636
14	23.637772533549363	19.918283963227783	22.781867493219682	33.662076010003176
15	29.991898841182064	24.35278785530626	19.61889331126061	26.036419992251066
16	26.00119756260787	23.947729914409496	21.996407312176395	28.05466521080624
17	26.98038110668874	23.961818886266776	21.295480962276777	27.762319044767708
18	26.163220738966576	21.323658905991337	23.507449543869534	29.005670811172557
19	27.952520164840973	22.23591983375013	23.25384805043852	26.557711950970376
20	29.847486879644958	20.161318727765842	22.982635342185905	27.008559050403296
21	27.39952801944278	22.17251946039238	22.1408192737135	28.28713324645134
22	28.424500722059808	22.570532915360502	21.193335916311508	27.811630446268182
23	27.99478708041281	22.429643196787715	22.197175161142617	27.378394561656865
24	28.628790813990353	21.006657039202565	22.27114226339333	28.093409883413756
25	27.864464090732977	22.193652918178298	22.48247684125251	27.459406149836212
26	27.938431192983693	23.105913845937092	22.186608432249656	26.76904652882956
27	27.73766334401747	21.2637807755979	23.877285055123103	27.12127082526153
28	26.240710084181607	22.517699270895704	22.26762002042901	28.973970624493678
29	28.02648726709168	23.22567010672396	21.992885069212072	26.75495755697228
30	26.63520129618541	22.743122820612165	23.232714592652602	27.388961290549823
31	28.05466521080624	22.58109964425346	22.80652319396992	26.557711950970376
32	25.77225176992709	22.404987496037478	22.845267866577437	28.977492867458
33	25.920185974428517	21.876651051389526	24.426754957556973	27.776408016624988
34	26.592934380613574	21.584304885350992	23.65890599133528	28.16385474270015
35	25.578528406889507	24.187242435983233	23.80684019583671	26.42738896129055
36	26.332288401253916	22.38737628121588	24.201331407840513	27.079003909689693
37	27.07548166672537	22.767778521362402	23.704695149871437	26.452044662040784
38	25.698284667676376	21.644183015744424	25.543305977246312	27.114226339332888
39	25.490472332781515	21.066535169595998	25.948363918143073	27.494628579479414
40	24.944524673311967	22.22183086189285	26.807791201437077	26.025853263358105
41	23.37008206826107	21.24969180374062	27.39952801944278	27.98069810855553
42	22.662111232432814	21.432848437885248	28.685146701419463	27.219893628262476
43	22.109119087034625	22.091507872213025	28.111021098235355	27.68835194251699
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.0
4	1.0
5	1.0
6	2.0
7	3.0
8	3.5
9	4.0
10	4.0
11	4.0
12	4.0
13	9.0
14	14.0
15	28.5
16	43.0
17	41.5
18	40.0
19	40.0
20	51.5
21	63.0
22	51.0
23	39.0
24	41.0
25	43.0
26	43.0
27	65.0
28	87.0
29	110.5
30	134.0
31	151.5
32	169.0
33	169.0
34	250.5
35	332.0
36	363.0
37	394.0
38	544.5
39	695.0
40	695.0
41	811.5
42	928.0
43	1058.5
44	1189.0
45	1602.5
46	2016.0
47	2016.0
48	2227.0
49	2438.0
50	2880.0
51	3322.0
52	3389.0
53	3456.0
54	3456.0
55	3158.5
56	2861.0
57	2720.0
58	2579.0
59	2305.0
60	2031.0
61	2031.0
62	1882.0
63	1733.0
64	1459.5
65	1186.0
66	1025.0
67	864.0
68	864.0
69	742.5
70	621.0
71	515.0
72	409.0
73	339.0
74	269.0
75	269.0
76	215.5
77	162.0
78	137.0
79	112.0
80	89.0
81	66.0
82	66.0
83	58.0
84	50.0
85	33.0
86	16.0
87	13.5
88	11.0
89	11.0
90	7.5
91	4.0
92	2.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	28391.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.21911873481032
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.44412782414004	59.8358634778627
2	6.467535110930185	8.953541615300624
3	2.4882963566049257	5.167130428656969
4	1.394260126195807	3.860378288894368
5	0.8446977406879707	2.923461660385333
6	0.6004477915733768	2.4937480187383327
7	0.40708324852432326	1.9724560600190202
8	0.2747811927539182	1.5216089605861012
9	0.1628332994097293	1.0144059737240676
>10	0.8650519031141869	10.115881793526118
>50	0.05088540606554041	2.141523722306365
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	78	0.274734951216935	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	70	0.24655700750237752	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	69	0.24303476453805783	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	67	0.23599027860941846	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	58	0.20429009193054137	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	57	0.20076784896622168	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	54	0.19020112007326265	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	52	0.1831566341446233	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	52	0.1831566341446233	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	51	0.1796343911803036	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	45	0.15850093339438556	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	42	0.14793420450142653	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	42	0.14793420450142653	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	38	0.1338452326441478	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	38	0.1338452326441478	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	37	0.1303229896798281	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	37	0.1303229896798281	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	33	0.1162340178225494	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	33	0.1162340178225494	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	32	0.11271177485822972	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	32	0.11271177485822972	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	32	0.11271177485822972	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	32	0.11271177485822972	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	31	0.10918953189391005	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	30	0.10566728892959036	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	30	0.10566728892959036	No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	29	0.10214504596527069	TruSeq Adapter, Index 3 (95% over 21bp)
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	29	0.10214504596527069	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.010566728892959036	0.0
23	0.0	0.0	0.0	0.010566728892959036	0.0
24	0.0	0.0	0.0	0.010566728892959036	0.0
25	0.0	0.0	0.0	0.014088971857278716	0.0
26	0.0	0.0	0.0	0.02113345778591807	0.0
27	0.0	0.0	0.0	0.04226691557183614	0.0
28	0.0	0.0	0.0	0.08805607410799197	0.0
29	0.0	0.0	0.0	0.22190130675213976	0.0
30	0.0	0.0	0.0	0.3592687823606072	0.0
31	0.0	0.0	0.0	0.8206826106864852	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATGAG	30	8.029485E-6	37.0	33
GAGCAAA	30	8.029485E-6	37.0	37
AATGAGC	30	8.029485E-6	37.0	34
TGAGCAA	35	2.3077757E-5	31.714285	36
CGGAGGG	30	3.512452E-4	30.833334	32
ATACGGA	30	3.512452E-4	30.833334	29
ACGGAGG	30	3.512452E-4	30.833334	31
GGAGGGT	25	0.005407511	29.6	33
GAGGGTG	25	0.005407511	29.6	34
GGGTGCA	25	0.005407511	29.6	36
GGTGCAA	25	0.005407511	29.6	37
GTATCAA	45	3.830137E-6	28.777777	2
GGTATCA	45	3.830137E-6	28.777777	1
ACGTCAA	40	5.741416E-5	27.75	29
TCAATGA	40	5.741416E-5	27.75	32
TCTGCGG	40	5.741416E-5	27.75	19
CTGCGGG	40	5.741416E-5	27.75	20
TGCGGGT	40	5.741416E-5	27.75	21
GTGCTTC	40	5.741416E-5	27.75	11
GGTAACG	40	5.741416E-5	27.75	25
>>END_MODULE