FastQCFastQC Report
Fri 10 Feb 2017
ERR1631763.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631763.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187274
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA22490.18942552435242413No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA22240.1873198604534421No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA21260.1790656579694325No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA20720.1745174239476313No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC18110.15253429284225883No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA17660.14874409782409115No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG17640.14857564471217258No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT17440.14689111359298695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17120.14419586380228996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16690.14057412189604085No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16390.1380473252172624No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC15200.12802436505810788No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA14310.12052820157773185No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT14250.12002284224197615No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13980.11774872523107555No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA13860.11673800655956418No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA11890.10014537503558571No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA405.9402904E-527.75000216
GGTATCA17550.027.3019941
GTCGAGA358.8695704E-426.428571
TCTATTA450.003825880820.55555737
TAGTACC450.003825880820.5555574
TTCTGCG6700.019.88059618
TGCGGGT6650.019.75187921
TATTCAA759.266198E-619.73333210
ATAGTAC759.266198E-619.7333323
CTTATAC12450.019.61445837
GTATCAA25650.018.6081872
CGTCTAT609.2370616E-418.517
GTAACGT6950.018.36690726
GCGGTAA8950.017.9832423
GGTAATA9150.017.7923525
ATACACA2500.017.7637
CAGTCGG3450.017.69565210
GCAGTCG3350.017.671649
TATATCA953.6065885E-617.5263162
TTTTACG953.6065885E-617.5263164
Produced by FastQC (version 0.11.5)