##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631763.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1187274
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62096281060648	34.0	31.0	34.0	31.0	34.0
2	32.791173730747914	34.0	31.0	34.0	31.0	34.0
3	32.879793543866036	34.0	31.0	34.0	31.0	34.0
4	36.28963491157054	37.0	37.0	37.0	35.0	37.0
5	36.213532006933534	37.0	35.0	37.0	35.0	37.0
6	36.268601013750825	37.0	37.0	37.0	35.0	37.0
7	36.25891327528439	37.0	37.0	37.0	35.0	37.0
8	36.2397820553638	37.0	37.0	37.0	35.0	37.0
9	38.07574578403974	39.0	38.0	39.0	37.0	39.0
10	38.0056718162783	39.0	38.0	39.0	35.0	39.0
11	38.08202908511431	39.0	38.0	39.0	37.0	39.0
12	38.017637040817874	39.0	38.0	39.0	35.0	39.0
13	38.06875076856732	39.0	38.0	39.0	37.0	39.0
14	39.46845631252769	40.0	39.0	41.0	37.0	41.0
15	39.47379374937883	41.0	39.0	41.0	37.0	41.0
16	39.42180659224408	40.0	39.0	41.0	37.0	41.0
17	39.393046592446225	40.0	39.0	41.0	37.0	41.0
18	39.41209190127974	41.0	39.0	41.0	37.0	41.0
19	39.4400138468458	41.0	39.0	41.0	37.0	41.0
20	39.424484996723585	41.0	39.0	41.0	37.0	41.0
21	39.393479516943856	40.0	39.0	41.0	37.0	41.0
22	39.36880366284446	40.0	39.0	41.0	36.0	41.0
23	39.30482095961	40.0	39.0	41.0	36.0	41.0
24	39.3185886324471	40.0	39.0	41.0	36.0	41.0
25	39.26501801606032	40.0	39.0	41.0	36.0	41.0
26	39.20571325574383	40.0	39.0	41.0	36.0	41.0
27	39.100391316578985	40.0	39.0	41.0	36.0	41.0
28	39.086932755202255	40.0	39.0	41.0	35.0	41.0
29	39.06090674941083	40.0	39.0	41.0	35.0	41.0
30	39.01377525322714	40.0	39.0	41.0	35.0	41.0
31	38.931933151067064	40.0	38.0	41.0	35.0	41.0
32	38.88483281870908	40.0	38.0	41.0	35.0	41.0
33	38.82748885261532	40.0	38.0	41.0	35.0	41.0
34	38.800362848003076	40.0	38.0	41.0	35.0	41.0
35	38.73180916957669	40.0	38.0	41.0	35.0	41.0
36	38.68395585180843	40.0	38.0	41.0	35.0	41.0
37	38.64270589602737	40.0	38.0	41.0	35.0	41.0
38	38.60402821926531	40.0	38.0	41.0	35.0	41.0
39	38.52923924890126	40.0	38.0	41.0	35.0	41.0
40	38.20776922597479	40.0	38.0	41.0	34.0	41.0
41	38.281901229202354	40.0	38.0	41.0	34.0	41.0
42	38.2732124176896	40.0	37.0	41.0	34.0	41.0
43	37.80705464787404	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	0.0
13	1.0
14	1.0
15	1.0
16	1.0
17	2.0
18	7.0
19	23.0
20	46.0
21	87.0
22	173.0
23	371.0
24	593.0
25	1007.0
26	1565.0
27	2512.0
28	3778.0
29	5492.0
30	7711.0
31	10768.0
32	14599.0
33	19942.0
34	28757.0
35	43673.0
36	67868.0
37	122728.0
38	281554.0
39	574011.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.835520696991594	16.718129092357785	12.857183767184324	27.589166443466297
2	20.283270753002256	19.741693998184076	33.25828747197362	26.716747776840055
3	21.05394374002968	20.296325869175945	29.561668157476706	29.088062233317668
4	15.339845730640105	16.131322676989473	34.34127252849806	34.18755906387237
5	16.736490481556913	33.20311907782029	33.50060727346847	16.559783167154336
6	33.50928260873227	34.14485620000101	15.732509934522277	16.61335125674444
7	30.008574263396653	28.193997341809897	20.262214114012437	21.535214280781016
8	27.10343189524912	31.297240569573663	20.31603488327042	21.283292651906805
9	28.118614574226335	13.316386950274325	18.095654415071838	40.469344060427495
10	19.093991782857202	23.94965273390978	30.162287728022342	26.794067755210676
11	38.119423149163545	20.157099372175253	19.454565668918885	22.268911809742317
12	22.51114738468121	24.268366021659702	27.122130190672078	26.09835640298701
13	33.97892988476123	18.274467393373392	21.63889717116689	26.10770555069849
14	23.781620754771012	20.500406814265286	23.707501385526847	32.01047104543686
15	28.952204798555346	24.710808120113807	20.70827795437279	25.628709126958054
16	25.16765295963695	24.565854217307884	23.004125416710885	27.26236740634428
17	26.280285763859055	24.229116446582676	22.504072353980632	26.986525435577637
18	25.944727164917282	22.199003768296112	24.22970603247439	27.626563034312213
19	27.512267597875468	22.895978518859167	24.17849628645115	25.413257596814216
20	28.963828063277724	21.543300030153105	23.739254797123493	25.75361710944567
21	26.791709411643815	23.031246367729775	22.687854699083783	27.489189521542627
22	27.50418184850338	23.512264228813233	22.192771003155126	26.790782919528265
23	26.990315630595802	22.998398010905653	23.285863246394683	26.72542311210386
24	27.182015271959127	22.56378898215576	23.518328540842298	26.735867205042812
25	27.11244413673676	22.687854699083783	23.821628368851673	26.378072795327785
26	27.158768742514365	23.311215439738426	23.16659844315634	26.36341737459087
27	26.671181210066088	22.390956089327315	24.361604819106624	26.576257881499977
28	25.790761020623716	23.416077501907733	23.089783824121472	27.70337765334708
29	26.701081637431628	23.787179707464325	23.16314515436201	26.348593500742034
30	26.18982644275879	22.97186664577848	24.283274122064494	26.555032789398236
31	27.08852379484432	23.011031994299547	23.556567397247814	26.343876813608315
32	24.946810929911713	23.003872737043007	23.869553279192505	28.179763053852774
33	25.34225461014054	22.56294671659617	25.26771410811658	26.827084565146713
34	25.61380102655326	22.5215072510642	24.746941312620336	27.11775040976219
35	25.399023308857092	23.79863451907479	24.974942599602112	25.827399572466003
36	25.571350842349787	22.79254830814117	25.443410703847636	26.192690145661405
37	26.171970412895423	22.296032760761207	24.966604170562146	26.565392655781228
38	25.334000407656532	21.793200221684295	26.111411519160697	26.761387851498476
39	24.802783519221343	20.98369879235964	26.958814898667033	27.25470278975199
40	24.456949280452534	21.88888158925404	27.69765024754185	25.956518882751578
41	23.055840522069886	21.10919636073897	28.66448688339844	27.170476233792705
42	21.847189444054194	21.461516044316646	29.286668452269655	27.40462605935951
43	21.186010979773833	22.10315394761445	29.042495666543694	27.668339406068014
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17.0
1	15.0
2	13.0
3	43.5
4	74.0
5	74.0
6	110.0
7	146.0
8	154.0
9	162.0
10	237.5
11	313.0
12	313.0
13	693.5
14	1074.0
15	2119.0
16	3164.0
17	3130.0
18	3096.0
19	3096.0
20	3518.0
21	3940.0
22	3465.5
23	2991.0
24	3404.5
25	3818.0
26	3818.0
27	4479.5
28	5141.0
29	7086.5
30	9032.0
31	10625.5
32	12219.0
33	12219.0
34	15224.0
35	18229.0
36	20583.0
37	22937.0
38	28804.5
39	34672.0
40	34672.0
41	40414.5
42	46157.0
43	51980.0
44	57803.0
45	73979.5
46	90156.0
47	90156.0
48	99948.0
49	109740.0
50	122670.5
51	135601.0
52	137814.0
53	140027.0
54	140027.0
55	125970.0
56	111913.0
57	104158.5
58	96404.0
59	87507.0
60	78610.0
61	78610.0
62	71220.5
63	63831.0
64	53060.0
65	42289.0
66	36593.5
67	30898.0
68	30898.0
69	26269.0
70	21640.0
71	18589.5
72	15539.0
73	12587.0
74	9635.0
75	9635.0
76	7949.0
77	6263.0
78	5059.5
79	3856.0
80	3139.5
81	2423.0
82	2423.0
83	1993.5
84	1564.0
85	1249.0
86	934.0
87	727.0
88	520.0
89	520.0
90	409.5
91	299.0
92	204.0
93	109.0
94	66.5
95	24.0
96	24.0
97	12.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1187274.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.92345147431202
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.95525452662187	38.61406705688836
2	6.709490925246121	6.02826979995262
3	2.16814338292326	2.9220145215631126
4	1.1126232350203347	1.9993150363051226
5	0.6921357630995925	1.554656368362023
6	0.4977898351250153	1.3417462501586641
7	0.33907797185901284	1.066278697037155
8	0.2672510798967492	0.9604672735359276
9	0.2258139929545524	0.9129909559243023
>10	1.5946495772701845	14.567449044840455
>50	0.24744228429590143	7.8344853983461835
>100	0.17186001229301898	14.741246077686979
>500	0.013567895555128821	4.155803361231739
>1k	0.004899517839352074	3.3012101581673408
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	2249	0.18942552435242413	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2224	0.1873198604534421	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2126	0.1790656579694325	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2072	0.1745174239476313	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1811	0.15253429284225883	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1766	0.14874409782409115	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1764	0.14857564471217258	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1744	0.14689111359298695	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1712	0.14419586380228996	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1669	0.14057412189604085	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1639	0.1380473252172624	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1520	0.12802436505810788	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1431	0.12052820157773185	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1425	0.12002284224197615	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1398	0.11774872523107555	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1386	0.11673800655956418	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1189	0.10014537503558571	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	8.422655595928152E-5	0.0	0.0	0.0	0.0
6	8.422655595928152E-5	0.0	0.0	0.0	0.0
7	8.422655595928152E-5	0.0	0.0	0.0	0.0
8	1.6845311191856303E-4	0.0	0.0	0.0	0.0
9	1.6845311191856303E-4	0.0	0.0	0.0	0.0
10	1.6845311191856303E-4	0.0	0.0	0.0	0.0
11	1.6845311191856303E-4	0.0	0.0	0.0	0.0
12	1.6845311191856303E-4	0.0	0.0	0.0	0.0
13	1.6845311191856303E-4	0.0	0.0	0.0	0.0
14	1.6845311191856303E-4	0.0	0.0	8.422655595928152E-5	0.0
15	1.6845311191856303E-4	0.0	0.0	4.211327797964076E-4	0.0
16	1.6845311191856303E-4	0.0	0.0	9.264921155520967E-4	0.0
17	1.6845311191856303E-4	0.0	0.0	0.0012633983393892227	0.0
18	3.3690622383712607E-4	0.0	0.0	0.0017687576751449117	0.0
19	3.3690622383712607E-4	0.0	0.0	0.0026110232347377267	0.0
20	3.3690622383712607E-4	0.0	8.422655595928152E-5	0.0033690622383712606	0.0
21	3.3690622383712607E-4	0.0	8.422655595928152E-5	0.0058116323611904245	0.0
22	3.3690622383712607E-4	0.0	8.422655595928152E-5	0.008591108707846714	0.0
23	3.3690622383712607E-4	0.0	8.422655595928152E-5	0.010444092938950907	0.0
24	3.3690622383712607E-4	0.0	8.422655595928152E-5	0.01221285061409582	0.0
25	3.3690622383712607E-4	0.0	8.422655595928152E-5	0.014318514513077857	0.0
26	3.3690622383712607E-4	0.0	8.422655595928152E-5	0.017856029863367682	0.0
27	3.3690622383712607E-4	0.0	8.422655595928152E-5	0.04084987964025153	0.0
28	3.3690622383712607E-4	0.0	8.422655595928152E-5	0.1298773492892121	0.0
29	3.3690622383712607E-4	0.0	8.422655595928152E-5	0.2711252836329272	0.0
30	3.3690622383712607E-4	0.0	8.422655595928152E-5	0.45406536317648666	0.0
31	3.3690622383712607E-4	0.0	8.422655595928152E-5	0.9761015570121134	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCTA	40	5.9402904E-5	27.750002	16
GGTATCA	1755	0.0	27.301994	1
GTCGAGA	35	8.8695704E-4	26.42857	1
TCTATTA	45	0.0038258808	20.555557	37
TAGTACC	45	0.0038258808	20.555557	4
TTCTGCG	670	0.0	19.880596	18
TGCGGGT	665	0.0	19.751879	21
TATTCAA	75	9.266198E-6	19.733332	10
ATAGTAC	75	9.266198E-6	19.733332	3
CTTATAC	1245	0.0	19.614458	37
GTATCAA	2565	0.0	18.608187	2
CGTCTAT	60	9.2370616E-4	18.5	17
GTAACGT	695	0.0	18.366907	26
GCGGTAA	895	0.0	17.98324	23
GGTAATA	915	0.0	17.79235	25
ATACACA	250	0.0	17.76	37
CAGTCGG	345	0.0	17.695652	10
GCAGTCG	335	0.0	17.67164	9
TATATCA	95	3.6065885E-6	17.526316	2
TTTTACG	95	3.6065885E-6	17.526316	4
>>END_MODULE