FastQCFastQC Report
Fri 10 Feb 2017
ERR1631762.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631762.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40146
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1230.3063817067702884No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1160.28894534947441836No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1060.2640362676231754No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA950.23663627758680814No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC910.22667264484631097No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT900.22418173666118668No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA840.20923628755044088No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG840.20923628755044088No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC820.20425447118019227No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT770.19179993025457082No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA770.19179993025457082No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA740.18432720569919792No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA740.18432720569919792No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA700.17436357295870075No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA690.17187266477357643No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA650.16190903203307927No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT640.15941812384795495No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT620.15443630747770637No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC590.1469635829223335No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA590.1469635829223335No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC580.14447267473720918No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC540.13450904199671201No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG540.13450904199671201No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA480.11956359288596621No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG480.11956359288596621No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT470.11707268470084194No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT470.11707268470084194No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC470.11707268470084194No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC460.11458177651571763No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC450.11209086833059334No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC440.10959996014546904No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA420.10461814377522044No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA420.10461814377522044No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG410.10212723559009614No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG410.10212723559009614No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT410.10212723559009614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG506.9649104E-933.30000327
CGCGGTA601.193257E-930.83333422
GTAATAC551.7631464E-830.27272826
CGGTAAT551.7631464E-830.27272824
GGTAATA551.7631464E-830.27272825
TGGTTCA250.005434097729.65
GTACTGG250.005434097729.61
AGTATTT250.005434097729.628
CTGGTTC250.005434097729.64
TCAGTCA250.005434097729.619
TACTGGT250.005434097729.62
ACTGGTT250.005434097729.63
GGAGTAT250.005434097729.626
GGTATCA652.8103386E-928.4615381
CCGCGGT652.8103386E-928.46153821
GCGGTAA604.1083695E-827.75000223
AATACGG604.1083695E-827.75000228
AGCCGCG706.195478E-926.4285719
AACTCCG658.9285095E-825.6153855
TAACTCC658.9285095E-825.6153854
Produced by FastQC (version 0.11.5)