##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631762.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	40146
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58546305983162	34.0	31.0	34.0	31.0	34.0
2	32.74585263787177	34.0	31.0	34.0	31.0	34.0
3	32.81883624769591	34.0	31.0	34.0	31.0	34.0
4	36.24475663827031	37.0	37.0	37.0	35.0	37.0
5	36.16716484830369	37.0	35.0	37.0	35.0	37.0
6	36.23723409555124	37.0	36.0	37.0	35.0	37.0
7	36.23247646091765	37.0	37.0	37.0	35.0	37.0
8	36.21608628505953	37.0	37.0	37.0	35.0	37.0
9	38.04451252926817	39.0	38.0	39.0	35.0	39.0
10	37.9709311014796	39.0	38.0	39.0	35.0	39.0
11	38.04453743835002	39.0	38.0	39.0	35.0	39.0
12	37.982663279031534	39.0	38.0	39.0	35.0	39.0
13	38.0581876152045	39.0	38.0	39.0	35.0	39.0
14	39.3898520400538	40.0	39.0	41.0	37.0	41.0
15	39.431126388681314	40.0	39.0	41.0	37.0	41.0
16	39.3726896826583	40.0	39.0	41.0	36.0	41.0
17	39.347780600807056	40.0	39.0	41.0	36.0	41.0
18	39.36745877546954	40.0	39.0	41.0	36.0	41.0
19	39.37037313804613	40.0	39.0	41.0	36.0	41.0
20	39.39488367458775	40.0	39.0	41.0	37.0	41.0
21	39.342350420963484	40.0	39.0	41.0	36.0	41.0
22	39.316270612265235	40.0	39.0	41.0	36.0	41.0
23	39.255143725402284	40.0	39.0	41.0	36.0	41.0
24	39.266078812334975	40.0	39.0	41.0	36.0	41.0
25	39.21239974094555	40.0	39.0	41.0	36.0	41.0
26	39.1573506700543	40.0	39.0	41.0	36.0	41.0
27	39.04981816370248	40.0	39.0	41.0	35.0	41.0
28	39.038036167986846	40.0	39.0	41.0	35.0	41.0
29	39.01242963184377	40.0	39.0	41.0	35.0	41.0
30	38.98271309719524	40.0	38.0	41.0	35.0	41.0
31	38.90138494495093	40.0	38.0	41.0	35.0	41.0
32	38.87929058934888	40.0	38.0	41.0	35.0	41.0
33	38.807876251681364	40.0	38.0	41.0	35.0	41.0
34	38.7574851790963	40.0	38.0	41.0	35.0	41.0
35	38.69655756488816	40.0	38.0	41.0	35.0	41.0
36	38.63445922383301	40.0	38.0	41.0	35.0	41.0
37	38.58760524087082	40.0	38.0	41.0	35.0	41.0
38	38.57746724455737	40.0	38.0	41.0	35.0	41.0
39	38.48121855228416	40.0	38.0	41.0	34.0	41.0
40	38.156404124943954	40.0	37.0	41.0	34.0	41.0
41	38.23048373436955	40.0	37.0	41.0	34.0	41.0
42	38.20368156229761	40.0	37.0	41.0	34.0	41.0
43	37.72388282767897	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	3.0
22	1.0
23	8.0
24	19.0
25	33.0
26	53.0
27	79.0
28	119.0
29	175.0
30	283.0
31	378.0
32	531.0
33	733.0
34	954.0
35	1606.0
36	2421.0
37	4287.0
38	9746.0
39	18716.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.97314800976436	16.093757784088076	12.658795396801672	28.274298809345886
2	21.62606486324914	19.356847506600907	31.881133861405868	27.135953768744088
3	21.573755791361528	19.47392019130175	29.427589299058436	29.524734718278285
4	16.038957804015343	15.98415782394261	34.434314751158276	33.542569620883775
5	17.11503013998904	32.441588203058835	33.19882429133662	17.244557365615503
6	32.66078812334977	34.36207841378967	15.657848851691329	17.319284611169234
7	29.704080107607233	27.551935435659843	19.864992776366265	22.87899168036666
8	26.724953918198572	30.513625267772632	20.903701489563094	21.857719324465698
9	28.24191700293927	12.716086285059532	17.386539132167588	41.6554575798336
10	19.997010910177853	22.368355502416183	29.562098341055147	28.07253524635082
11	39.84954914561849	19.000647636128132	18.781447715837192	22.368355502416183
12	22.667264484631097	23.723409555123798	26.69755392816221	26.911772032082897
13	35.53529616898321	17.824938972749464	20.61226523190355	26.02749962636377
14	23.907736760822996	20.148956309470435	23.080755243361732	32.862551686344844
15	30.506152543217258	23.469336920241123	19.424102027599265	26.60040850894236
16	25.100881781497534	23.66611866686594	22.642355402779852	28.590644148856676
17	27.048771982264736	23.38215513376177	21.349574054700344	28.219498829273153
18	26.59542669257211	21.003337816968067	23.00104618143775	29.400189309022068
19	28.082498879091318	22.06197379564589	23.252627908135306	26.602899417127485
20	30.284461714741195	20.333283515169633	22.6573008518906	26.724953918198572
21	27.571862701140837	21.43426493299457	21.62606486324914	29.367807502615456
22	28.518407811488068	22.90639167040303	20.868828774971355	27.70637174313755
23	28.906989488367458	21.740646639764858	21.59617396502765	27.756189906840035
24	28.408807851342598	21.18517411448214	22.283664624121954	28.122353410053307
25	28.550789617894683	21.900064763612814	22.38579185971205	27.163353758780453
26	28.351516963084737	22.707119015593086	21.887610222687194	27.053753798634983
27	27.68644447765655	21.14531958352015	24.455736561550342	26.71249937727295
28	26.436008568724155	23.304936980022916	21.419319483883825	28.8397349673691
29	28.329098789418623	23.73088227967917	21.18019229811189	26.75982663279032
30	26.361281323170427	22.433119115229413	23.887809495342	27.31779006625816
31	28.17217157375579	21.974792009166542	22.91884621132865	26.934190205749015
32	25.87804513525632	21.401883126587954	22.751955362925322	29.96811637523041
33	26.388681313206796	21.262392268220992	24.366063866885867	27.982862551686345
34	26.13211777013899	21.792955711652468	23.828027698899017	28.246898819309518
35	25.653863398595128	23.531609624869226	24.56533652169581	26.249190454839834
36	26.109699596472876	22.55268270811538	24.789518258356996	26.548099437054752
37	27.589299058436705	21.63602849598964	24.27639117222139	26.498281273352266
38	26.523190355203507	21.15528321626065	24.772081901061128	27.549444527474716
39	25.26029990534549	20.462810740796094	26.12962686195387	28.147262491904552
40	25.26279081353061	22.007173815573157	26.81711752104817	25.912917849848053
41	23.49922781846261	20.637174313754794	28.00278981716734	27.860808050615255
42	21.563792158621034	21.379464952921836	29.04149853036417	28.01524435809296
43	21.14531958352015	21.910028396353308	28.443680565934336	28.500971454192197
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.0
6	2.0
7	2.0
8	4.5
9	7.0
10	5.5
11	4.0
12	4.0
13	14.5
14	25.0
15	47.0
16	69.0
17	58.0
18	47.0
19	47.0
20	62.5
21	78.0
22	63.0
23	48.0
24	50.5
25	53.0
26	53.0
27	58.0
28	63.0
29	93.5
30	124.0
31	152.5
32	181.0
33	181.0
34	232.0
35	283.0
36	328.0
37	373.0
38	520.5
39	668.0
40	668.0
41	805.0
42	942.0
43	1175.5
44	1409.0
45	2190.5
46	2972.0
47	2972.0
48	3434.0
49	3896.0
50	4405.0
51	4914.0
52	5070.0
53	5226.0
54	5226.0
55	4865.0
56	4504.0
57	4299.5
58	4095.0
59	3663.5
60	3232.0
61	3232.0
62	2870.5
63	2509.0
64	2027.0
65	1545.0
66	1291.0
67	1037.0
68	1037.0
69	867.0
70	697.0
71	601.0
72	505.0
73	368.5
74	232.0
75	232.0
76	195.0
77	158.0
78	131.0
79	104.0
80	84.5
81	65.0
82	65.0
83	51.5
84	38.0
85	26.5
86	15.0
87	15.0
88	15.0
89	15.0
90	12.0
91	9.0
92	4.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	40146.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.61854232053007
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.25755322313347	48.80436407114034
2	7.325882802872561	8.588651422308574
3	2.9363022139123784	5.163652667762666
4	1.6487485658437089	3.865889503312908
5	1.113330217141886	3.263089722512828
6	0.7138911316024306	2.5108354506052906
7	0.5949092763353588	2.4410900214218105
8	0.33569880593209533	1.5742539729985552
9	0.2719585263247355	1.4347631146315947
>10	1.7039901415034209	17.90215712648832
>50	0.08498703947647984	3.591889602949235
>100	0.012748055921471975	0.8593633238678823
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	123	0.3063817067702884	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	116	0.28894534947441836	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	106	0.2640362676231754	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	95	0.23663627758680814	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	91	0.22667264484631097	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	90	0.22418173666118668	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	84	0.20923628755044088	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	84	0.20923628755044088	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	82	0.20425447118019227	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	77	0.19179993025457082	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	77	0.19179993025457082	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	74	0.18432720569919792	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	74	0.18432720569919792	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	70	0.17436357295870075	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	69	0.17187266477357643	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	65	0.16190903203307927	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	64	0.15941812384795495	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	62	0.15443630747770637	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	59	0.1469635829223335	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	59	0.1469635829223335	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	58	0.14447267473720918	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	54	0.13450904199671201	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	54	0.13450904199671201	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	48	0.11956359288596621	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	48	0.11956359288596621	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	47	0.11707268470084194	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	47	0.11707268470084194	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	47	0.11707268470084194	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	46	0.11458177651571763	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	45	0.11209086833059334	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	44	0.10959996014546904	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	42	0.10461814377522044	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	42	0.10461814377522044	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	41	0.10212723559009614	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	41	0.10212723559009614	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	41	0.10212723559009614	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.002490908185124296	0.0	0.0	0.002490908185124296	0.0
22	0.002490908185124296	0.0	0.0	0.004981816370248592	0.0
23	0.002490908185124296	0.0	0.0	0.009963632740497184	0.0
24	0.002490908185124296	0.0	0.0	0.014945449110745778	0.0
25	0.002490908185124296	0.0	0.0	0.014945449110745778	0.0
26	0.002490908185124296	0.0	0.0	0.017436357295870075	0.0
27	0.002490908185124296	0.0	0.0	0.03985453096198874	0.0
28	0.002490908185124296	0.0	0.0	0.11209086833059334	0.0
29	0.002490908185124296	0.0	0.0	0.2590544512529268	0.0
30	0.002490908185124296	0.0	0.0	0.44587256513724904	0.0
31	0.002490908185124296	0.0	0.0	0.9390723857918597	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	50	6.9649104E-9	33.300003	27
CGCGGTA	60	1.193257E-9	30.833334	22
GTAATAC	55	1.7631464E-8	30.272728	26
CGGTAAT	55	1.7631464E-8	30.272728	24
GGTAATA	55	1.7631464E-8	30.272728	25
TGGTTCA	25	0.0054340977	29.6	5
GTACTGG	25	0.0054340977	29.6	1
AGTATTT	25	0.0054340977	29.6	28
CTGGTTC	25	0.0054340977	29.6	4
TCAGTCA	25	0.0054340977	29.6	19
TACTGGT	25	0.0054340977	29.6	2
ACTGGTT	25	0.0054340977	29.6	3
GGAGTAT	25	0.0054340977	29.6	26
GGTATCA	65	2.8103386E-9	28.461538	1
CCGCGGT	65	2.8103386E-9	28.461538	21
GCGGTAA	60	4.1083695E-8	27.750002	23
AATACGG	60	4.1083695E-8	27.750002	28
AGCCGCG	70	6.195478E-9	26.42857	19
AACTCCG	65	8.9285095E-8	25.615385	5
TAACTCC	65	8.9285095E-8	25.615385	4
>>END_MODULE