FastQCFastQC Report
Fri 10 Feb 2017
ERR1631761.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631761.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420444
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA7710.18337757228073182No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA7500.17838285241316323No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6560.15602553491071344No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG6440.15317140927210282No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6360.15126865884636242No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6250.14865237701096934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5960.1417549067176604No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5930.14104137530800773No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5600.13319252980182855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5580.13271684219539343No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5500.13081409176965303No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC5260.1251058404924318No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4900.11654346357659996No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4840.11511640075729467No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4510.10726755525111548No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4250.10108361636745916No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATT303.5956307E-430.8333322
GATTAAT250.005492426529.61
ATACCAT250.005492426529.66
ATACGGC454.0003106E-628.77777729
GGTATCA5550.027.01
TGTATTC358.8600034E-426.428573
TTAGAAC951.0913936E-1125.315793
GCGGTAA3200.024.2812523
CGTTAGA1002.0008883E-1124.0500011
CTTATAC4050.023.29629737
CGCGGTA3350.023.1940322
TAGCGAA400.001929161523.12510
GTAGCGA400.001929161523.1259
TAATACG3200.022.54687527
AGAACAT1250.022.1999995
TAATACT603.7190555E-521.5833324
CGGTAAT3650.021.2876724
GTAATAC3700.021.026
CCGCGGT3700.021.021
ACGGTAC806.938535E-720.81253
Produced by FastQC (version 0.11.5)