FastQCFastQC Report
Fri 10 Feb 2017
ERR1631760.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631760.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108456
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2400.22128789555211328No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2240.2065353691819724No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2240.2065353691819724No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2070.19086080991369772No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1900.175186250645423No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1750.16135575717341594No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1730.15951169137714832No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1630.15029136239581029No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1540.14199306631260603No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1510.1392269676182046No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1430.13185070443313418No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1360.12539647414619753No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1340.12355240834992992No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1260.1161761451648595No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1240.11433207936859185No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1120.1032676845909862No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG200.001834596237.01
CCGAGTT200.001834596237.016
GGTATCA1900.030.1842121
CCTAGTT250.00547469429.5999982
TGGGTTC250.00547469429.59999826
TAGTTCC250.00547469429.5999984
AGTTCCT250.00547469429.5999985
AATACGG652.926754E-928.46153828
TAATACG706.4519554E-926.42857227
AACGCGT358.817487E-426.42857226
TCTTATA1500.024.66666637
CTTATAC551.882144E-523.54545437
ATCGCCT551.882144E-523.54545417
CCGATAG400.001919992223.12500227
CGATAGT400.001919992223.12500228
ACGCGTT400.001919992223.12500227
ATACGGA652.6458401E-622.7692329
GTATCAA2550.022.4901962
ATTAGCA603.686391E-521.5833342
GATTAGC603.686391E-521.5833341
Produced by FastQC (version 0.11.5)