FastQCFastQC Report
Fri 10 Feb 2017
ERR1631759.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631759.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270912
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25340.19938437909155002No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25130.1977320223587471No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19430.1528823396112398No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA19090.15020709537717794No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA18830.1481613203746601No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG16960.13344747708731997No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA16810.13226722227817506No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA15750.12392675496021754No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC15620.12290386745895861No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA15370.12093677611038373No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT14960.11771074629872093No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14940.11755337899083493No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC14040.11047185013596536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16650.026.01
TAGACTC705.1026236E-621.1428575
ATAAGAT555.1435054E-420.1818183
GACCGTT1650.020.1818167
GCGGTAA6400.019.07812523
CTTATAC10650.018.58685537
GGGTATT500.0070352818.51
AGTCGGT3800.018.511
AACTCCG6750.018.088895
AGGACCG2800.017.8392855
CGGTAAT6750.017.81481624
TAATACG6350.017.48031427
GTACTGG5450.017.3119261
TACCGCT752.0674826E-417.26666635
TCTTATA19450.017.21593937
CAGTCGG4300.017.20930310
GCAGTCG4100.017.1463419
TTAGACT650.00158005817.0769234
CGTTACG650.00158005817.07692316
GGACCGT2600.017.0769236
Produced by FastQC (version 0.11.5)