##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631759.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1270912
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.63427995014604	34.0	31.0	34.0	31.0	34.0
2	32.80143865192869	34.0	31.0	34.0	31.0	34.0
3	32.89120411169302	34.0	31.0	34.0	31.0	34.0
4	36.304261034595626	37.0	37.0	37.0	35.0	37.0
5	36.22331758611139	37.0	35.0	37.0	35.0	37.0
6	36.27993676981569	37.0	37.0	37.0	35.0	37.0
7	36.26376963944002	37.0	37.0	37.0	35.0	37.0
8	36.245435561234764	37.0	37.0	37.0	35.0	37.0
9	38.07786927812469	39.0	38.0	39.0	37.0	39.0
10	38.01227701052473	39.0	38.0	39.0	35.0	39.0
11	38.08445352628664	39.0	38.0	39.0	37.0	39.0
12	38.0245611025783	39.0	38.0	39.0	35.0	39.0
13	38.07359990306174	39.0	38.0	39.0	37.0	39.0
14	39.47965004658072	41.0	39.0	41.0	37.0	41.0
15	39.48224031246853	41.0	39.0	41.0	37.0	41.0
16	39.43731509341323	41.0	39.0	41.0	37.0	41.0
17	39.407971598348276	41.0	39.0	41.0	37.0	41.0
18	39.424807539782456	41.0	39.0	41.0	37.0	41.0
19	39.45285432823044	41.0	39.0	41.0	37.0	41.0
20	39.436151362171415	41.0	39.0	41.0	37.0	41.0
21	39.40544034520093	41.0	39.0	41.0	37.0	41.0
22	39.37888225148555	40.0	39.0	41.0	36.0	41.0
23	39.31973496198006	40.0	39.0	41.0	36.0	41.0
24	39.338334204099105	41.0	39.0	41.0	36.0	41.0
25	39.27370030340417	40.0	39.0	41.0	36.0	41.0
26	39.218091417816495	40.0	39.0	41.0	36.0	41.0
27	39.12304077701682	40.0	39.0	41.0	36.0	41.0
28	39.11151204804109	40.0	39.0	41.0	35.0	41.0
29	39.08589422399033	40.0	39.0	41.0	35.0	41.0
30	39.034614513042605	40.0	39.0	41.0	35.0	41.0
31	38.943082605247255	40.0	38.0	41.0	35.0	41.0
32	38.891084512539024	40.0	38.0	41.0	35.0	41.0
33	38.82902514100111	40.0	38.0	41.0	35.0	41.0
34	38.79492757956491	40.0	38.0	41.0	35.0	41.0
35	38.72378654068889	40.0	38.0	41.0	35.0	41.0
36	38.673192951203546	40.0	38.0	41.0	35.0	41.0
37	38.629894910111794	40.0	38.0	41.0	35.0	41.0
38	38.5827106833518	40.0	38.0	41.0	35.0	41.0
39	38.51453837873905	40.0	38.0	41.0	35.0	41.0
40	38.19002653212811	40.0	38.0	41.0	34.0	41.0
41	38.258637891529865	40.0	38.0	41.0	34.0	41.0
42	38.24040295472857	40.0	37.0	41.0	34.0	41.0
43	37.77428177560681	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	0.0
13	0.0
14	0.0
15	1.0
16	3.0
17	1.0
18	10.0
19	23.0
20	43.0
21	95.0
22	199.0
23	362.0
24	632.0
25	1147.0
26	1752.0
27	2782.0
28	4171.0
29	6105.0
30	8525.0
31	11679.0
32	15671.0
33	21355.0
34	31107.0
35	46240.0
36	71860.0
37	130172.0
38	292957.0
39	624017.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.71806387853761	17.110704753751637	12.811902004230033	27.35932936348071
2	19.735670132943902	19.760140749320172	34.06593060731192	26.438258510424014
3	20.424230788599054	20.77460909960721	29.6487089586061	29.15245115318763
4	15.118749370530768	16.112209185215026	34.28356959411824	34.485471850135966
5	16.325677938362375	33.59099607211199	33.824371789706916	16.258954199818714
6	33.85663208782355	34.247689847920235	15.654742421190452	16.240935643065768
7	29.982957120555948	28.548475425521204	20.648400518682646	20.820166935240206
8	26.85984552825058	32.03715127404572	20.179131206566623	20.923871991137073
9	27.78099506496122	13.688831327424717	18.56477867861819	39.96539492899587
10	18.71238921341525	24.514993957095378	30.77396389364488	25.998652935844497
11	37.36033651425119	20.64273529559875	19.980927082284218	22.016001107865847
12	22.584883925873704	24.49744750226609	27.331317982677007	25.586350589183198
13	32.87285036257428	18.585944581528853	22.39195160640548	26.14925344949139
14	23.558672827072215	20.48961690502568	24.055717469030114	31.89599279887199
15	28.097224670158123	25.31080043307483	21.30336325410414	25.288611642662907
16	25.20080068486252	24.83743957095377	23.21742181992144	26.74433792426226
17	25.710277344143417	24.73861290160137	23.220333115117334	26.33077663913788
18	25.44416922650821	22.935813022459463	24.94562959512539	26.674388155906943
19	26.927434786987615	23.331827852754557	24.76646691509719	24.97427044516064
20	27.79822678517474	22.43341789203344	24.485959688790413	25.28239563400141
21	26.051685718602073	23.732642385940174	23.598880174237085	26.616791721220668
22	26.75810756370229	23.97837143720415	23.030390774498944	26.23313022459462
23	26.299224493906735	23.627914442542046	23.964680481418068	26.108180582133144
24	26.77337219256723	23.297600463289356	23.82792829086514	26.101099053278276
25	26.57886620002014	23.226234389163057	24.387762488669555	25.807136922147244
26	26.368308742068685	23.91455899385638	23.876633472655858	25.840498791419076
27	26.11046240809749	23.079646741867258	24.565115444657064	26.244775405378185
28	25.20363329640447	23.882613430355523	24.006068083392083	26.907685189847918
29	26.068760071507707	24.335280491489574	23.983643242018328	25.61231619498439
30	25.6099556853661	23.60273567328029	24.833898806526335	25.953409834827273
31	26.342264452613556	23.65608319065364	24.229687027898077	25.771965328834728
32	24.479664996474973	23.61674136368214	24.43316295699466	27.470430682848225
33	24.872532480612346	23.168401903514958	25.683918320072515	26.27514729580018
34	25.33818234464699	23.060054512035453	25.200879368516464	26.400883774801088
35	25.13273932420183	24.26658966159734	25.155085481921645	25.445585532279182
36	25.273347013797963	23.451033588478197	25.541579590089636	25.7340398076342
37	25.594612372847216	22.86484036660288	25.3420378436902	26.198509416859707
38	24.928240507603988	22.540506345049856	26.219281901500658	26.311971245845506
39	24.416718073320574	21.95148051163259	27.11729844395206	26.51450297109477
40	24.205295095175746	22.819282530969886	27.45461526840568	25.520807105448682
41	22.950841600362573	22.0131684963239	28.444927736932218	26.591062166381306
42	22.18155151576191	22.401551012186523	28.644941585255314	26.771955886796256
43	21.686710016114414	22.745083845301643	28.637466638130725	26.930739500453214
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	21.0
2	28.0
3	74.0
4	120.0
5	120.0
6	176.0
7	232.0
8	247.0
9	262.0
10	394.5
11	527.0
12	527.0
13	1103.0
14	1679.0
15	3283.5
16	4888.0
17	4777.5
18	4667.0
19	4667.0
20	5415.0
21	6163.0
22	5556.5
23	4950.0
24	5445.5
25	5941.0
26	5941.0
27	6953.0
28	7965.0
29	10602.5
30	13240.0
31	15731.0
32	18222.0
33	18222.0
34	22287.0
35	26352.0
36	29724.0
37	33096.0
38	39886.5
39	46677.0
40	46677.0
41	52790.0
42	58903.0
43	64636.5
44	70370.0
45	83222.5
46	96075.0
47	96075.0
48	103541.0
49	111007.0
50	122780.5
51	134554.0
52	134720.0
53	134886.0
54	134886.0
55	121524.0
56	108162.0
57	100259.0
58	92356.0
59	84077.0
60	75798.0
61	75798.0
62	69435.5
63	63073.0
64	53480.0
65	43887.0
66	38475.5
67	33064.0
68	33064.0
69	28612.5
70	24161.0
71	20907.0
72	17653.0
73	14746.0
74	11839.0
75	11839.0
76	9832.5
77	7826.0
78	6399.5
79	4973.0
80	3958.5
81	2944.0
82	2944.0
83	2407.0
84	1870.0
85	1565.0
86	1260.0
87	981.0
88	702.0
89	702.0
90	545.5
91	389.0
92	251.5
93	114.0
94	66.5
95	19.0
96	19.0
97	11.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1270912.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.02529768624614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.41492585400613	40.1666231513328
2	7.13435559465191	6.709903912760834
3	2.328892831815674	3.2855063608649044
4	1.2058140919928568	2.2681506652093875
5	0.7245202012391551	1.7035389071485114
6	0.4966647233993245	1.4013483880866202
7	0.33483028680037485	1.1021845737811162
8	0.25707440269942383	0.9671200251563461
9	0.20830223068804776	0.8815926966133105
>10	1.4946143014688156	14.237782929355603
>50	0.22342541167890773	7.3843426088725055
>100	0.1649651754965539	14.909667958231454
>500	0.008753253496696367	2.788596663712821
>1k	0.0028616405662276584	2.193641158873899
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2534	0.19938437909155002	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2513	0.1977320223587471	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1943	0.1528823396112398	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1909	0.15020709537717794	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1883	0.1481613203746601	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1696	0.13344747708731997	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1681	0.13226722227817506	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1575	0.12392675496021754	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1562	0.12290386745895861	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1537	0.12093677611038373	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1496	0.11771074629872093	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1494	0.11755337899083493	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1404	0.11047185013596536	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	7.868365394299526E-5	0.0	0.0	0.0	0.0
3	7.868365394299526E-5	0.0	0.0	0.0	0.0
4	7.868365394299526E-5	0.0	0.0	0.0	0.0
5	7.868365394299526E-5	0.0	0.0	0.0	0.0
6	7.868365394299526E-5	0.0	0.0	0.0	0.0
7	7.868365394299526E-5	0.0	0.0	0.0	0.0
8	2.360509618289858E-4	0.0	0.0	0.0	0.0
9	2.360509618289858E-4	0.0	0.0	7.868365394299526E-5	0.0
10	2.360509618289858E-4	0.0	0.0	7.868365394299526E-5	7.868365394299526E-5
11	2.360509618289858E-4	0.0	0.0	7.868365394299526E-5	7.868365394299526E-5
12	2.360509618289858E-4	0.0	0.0	7.868365394299526E-5	7.868365394299526E-5
13	3.1473461577198104E-4	0.0	0.0	7.868365394299526E-5	7.868365394299526E-5
14	3.1473461577198104E-4	0.0	0.0	7.868365394299526E-5	7.868365394299526E-5
15	3.1473461577198104E-4	0.0	0.0	2.360509618289858E-4	7.868365394299526E-5
16	3.1473461577198104E-4	0.0	0.0	3.9341826971497635E-4	7.868365394299526E-5
17	3.1473461577198104E-4	0.0	0.0	5.507855776009669E-4	7.868365394299526E-5
18	4.721019236579716E-4	0.0	0.0	9.442038473159432E-4	7.868365394299526E-5
19	4.721019236579716E-4	0.0	0.0	0.0013376221170309196	7.868365394299526E-5
20	4.721019236579716E-4	0.0	0.0	0.0022031423104038675	7.868365394299526E-5
21	4.721019236579716E-4	0.0	0.0	0.0036981317353207777	7.868365394299526E-5
22	4.721019236579716E-4	0.0	0.0	0.005507855776009669	1.5736730788599052E-4
23	5.507855776009669E-4	0.0	0.0	0.006452059623325612	1.5736730788599052E-4
24	5.507855776009669E-4	0.0	0.0	0.008261783664014503	1.5736730788599052E-4
25	5.507855776009669E-4	0.0	0.0	0.009756773088931412	1.5736730788599052E-4
26	6.294692315439621E-4	0.0	0.0	0.012510700976936247	1.5736730788599052E-4
27	6.294692315439621E-4	0.0	0.0	0.028168748111592305	1.5736730788599052E-4
28	6.294692315439621E-4	0.0	0.0	0.10000692416154698	1.5736730788599052E-4
29	6.294692315439621E-4	0.0	0.0	0.2140195387249471	1.5736730788599052E-4
30	6.294692315439621E-4	0.0	0.0	0.3558074831302246	1.5736730788599052E-4
31	6.294692315439621E-4	0.0	0.0	0.7987177711753449	1.5736730788599052E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1665	0.0	26.0	1
TAGACTC	70	5.1026236E-6	21.142857	5
ATAAGAT	55	5.1435054E-4	20.181818	3
GACCGTT	165	0.0	20.181816	7
GCGGTAA	640	0.0	19.078125	23
CTTATAC	1065	0.0	18.586855	37
GGGTATT	50	0.00703528	18.5	1
AGTCGGT	380	0.0	18.5	11
AACTCCG	675	0.0	18.08889	5
AGGACCG	280	0.0	17.839285	5
CGGTAAT	675	0.0	17.814816	24
TAATACG	635	0.0	17.480314	27
GTACTGG	545	0.0	17.311926	1
TACCGCT	75	2.0674826E-4	17.266666	35
TCTTATA	1945	0.0	17.215939	37
CAGTCGG	430	0.0	17.209303	10
GCAGTCG	410	0.0	17.146341	9
TTAGACT	65	0.001580058	17.076923	4
CGTTACG	65	0.001580058	17.076923	16
GGACCGT	260	0.0	17.076923	6
>>END_MODULE