FastQCFastQC Report
Fri 10 Feb 2017
ERR1631758.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631758.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52950
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1250.2360717658168083No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1210.22851746931067043No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1180.22285174693106705No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1130.2134088762983947No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA980.1850802644003777No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG960.1813031161473088No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT940.17752596789423986No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC890.16808309726156753No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT880.16619452313503305No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA860.16241737488196412No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA730.13786591123701605No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA700.13220018885741266No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA700.13220018885741266No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA690.13031161473087818No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA630.11898016997167139No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT590.11142587346553352No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA570.10764872521246459No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT550.10387157695939567No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA540.10198300283286119No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT530.10009442870632672No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT530.10009442870632672No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTCA250.00544970529.65
GTACTGG250.00544970529.61
ACTCAGA250.00544970529.633
TACTGGT250.00544970529.62
ACTGGTT358.757686E-426.4285723
TGAACTC400.001907094523.12530
TCTTATA704.9520277E-621.14285937
TGGGGCT450.003778531720.55555536
CTCTTAT1457.2759576E-1219.1379337
GGTATCA752.0207505E-417.2666661
GACATTG855.245164E-415.23529431
ACGACAT855.245164E-415.23529429
ACATTGG908.0917927E-414.38888832
CGACATT908.0917927E-414.38888830
TGGTCAC800.00619461613.87537
CATTGGG850.00925071913.05882433
ATTGGGG850.00925071913.05882434
AACGACA1000.001792701412.95000128
GTATCAA1000.001792701412.9500011
TTGGGGC1000.001792701412.95000135
Produced by FastQC (version 0.11.5)