FastQCFastQC Report
Fri 10 Feb 2017
ERR1631757.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631757.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1346270
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT79680.5918575025812058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT76940.5715049729994726No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT61950.4601602947402824No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA21480.1595519472319817No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20160.1497470789663292No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA19870.14759297912008734No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA18580.1380109487695633No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA17570.1305087389602383No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA16180.12018391555928604No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG16170.1201096362542432No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA15620.116024274476888No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT14540.10800210953226322No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC14440.10725931648183498No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCG303.5998033E-430.83333418
GGTATCA28150.027.4049721
ATAACGT754.620233E-1027.13333313
CATAACG809.786163E-1025.43750212
AACGTCG1005.347829E-1022.215
CCGCATA1005.347829E-1022.29
ACGTCGC1052.2602762E-819.3809516
CGTCGCA1052.2602762E-819.3809517
ACTAGAC609.2379353E-418.53
ATACCGC1103.8529834E-818.56
GTATCAA43000.017.8116282
GTATAGA650.001580123917.0769231
CGCATAA1201.0415533E-716.95833410
GTAACGT6900.016.89130426
CTTATAC12500.016.87200237
TATACTT1107.810049E-716.8181825
TAAGGTA1452.9813236E-916.5862065
GTCGCAA1352.2200766E-816.44444518
GTTTAGG1352.2200766E-816.4444451
TACCGCA1708.54925E-1116.323537
Produced by FastQC (version 0.11.5)