FastQCFastQC Report
Fri 10 Feb 2017
ERR1631756.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631756.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309572
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA8080.26100551729484583No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA7910.25551406457948395No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7090.22902588089362086No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6960.22482653469952063No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6570.2122284961172199No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6540.2112594162262737No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC6270.2025376972077578No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6240.2015686173168116No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6130.19801532438334216No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5970.1928468982982957No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5170.16700476787306345No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5120.16538963472148643No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4900.15828304885454758No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4520.14600803690256225No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4510.14568501027224684No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4340.140193557556885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4270.13793237114467718No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4190.13534815810215395No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4170.13470210484152315No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA4050.1308257852777383No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA4040.13050275864742292No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3990.1288876254958459No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC3910.12630341245332266No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3750.1211349863682762No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3580.11564353365291434No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3570.11532050702259894No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3460.1117672140891295No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3380.10918300104660629No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3350.10821392115566007No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3350.10821392115566007No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT3340.10789089452534467No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3300.10659878800408305No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3270.10562970811313685No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG3160.10207641517966741No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3140.10143036191903661No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA3140.10143036191903661No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA3130.1011073352887212No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGAC200.001839859437.03
CGATCGC200.001839859437.019
GGTATCA5500.031.9545461
TCCTCCG250.005490217429.621
GTCTAGA250.005490217429.61
CCGCTTA250.005490217429.625
CAGTCGG1650.024.66666610
GCAGTCG1700.023.9411779
AGTCGGT1700.023.94117711
TCTATGG2050.023.4634152
TGCTTGG400.001928018223.1252
CTAGTTC400.001928018223.1253
TATACAC652.6723446E-622.7692337
GTATCAA7750.022.438712
CTATGGG2200.021.8636363
GATTCCT1800.021.58333218
CTCTATG2150.021.5116271
GGGCAGT2000.021.2750027
TATGGGC2300.020.9130444
CTTATTG1700.020.67647228
Produced by FastQC (version 0.11.5)